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The paper presents the results of testing eggs for the content of dioxins and polychlorinated biphenyls (PCBs), determination of the sources that caused the presence of high concentrations of these compounds which exceeded the acceptable contamination levels, and assessment of consumer health risk caused by the consumption of eggs with excessive contents of investigated compounds. In about 9% of free range eggs and 17% of organic eggs, the content of PCDD/Fs was two- or threefold higher than the acceptable limits, and in some samples the concentration of investigated compounds exceeded the maximum concentration levels. Based on the profile of the compounds, it was confirmed in several cases that their main source was the soil or unsecured refuse. The consumers of eggs and meat produced under these conditions constitute the risk groups, and their dioxin and PCB intake may exceed toxicological reference values.
The study was to examine the incidence of keratinolytic fungi in the lagoon with acidic petroleum wastes (before bioremediation) at a petroleum refinery situated within a highly populated area. High concentrations of petroleum hydrocarbons (non-polar and polar aliphatics and PAHs) made the growth of keratinolytic fungi in clay and litter from the lagoon impossible. The natural self-purification process considerably decreased the hydrocarbon contamination, increased the pH and caused the abundant growth of Trichophyton ajelloi in organic soil. This soil included the root-adjacent material from the grass growing in several green oases at the lagoon. It is possible that the strains of Trichophyton ajelloi adapted to the extreme lagoon's conditions actively participated in the soil self-purification process. The fungal species isolated in the present study did not pose a threat to public health. However, petroleum contaminants may support the growth of potential pathogens, for example Microsporum gypseum, in other types of soils.
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Chromosomal aberrations in humans induced by benzene

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Adverse effects associated with occupational exposure to benzene have often been reported in humans. It has been shown, that benzene causes chromosomal aberrations, sister chromatid exchanges and micronuclei in lymphocytes of exposed workers. In addition to evidence by conventional cytogenetic methods, the genotoxic effect of benzene has also been proved by a more specific approach based on fluorescence in situ hybridization with DNA probes. In the present paper, the nature of benzene-induced chromosomal aberrations and supposed consequence on human health is reviewed. The new possibilities in chromosomal alterations identification by molecular cytogenetic methods are also presented.
For proper growth and development, rhododendrons need acidic soils, whereas calcium carbonate (CaCO3) in the substrate markedly limits their growth. In this study, we analysed the reactions of rhododendrons to high concentrations of calcium salts and pH in the substrate. We used 4-month-old seedlings of Rhododendron ‘Cunningham’s White’ and 1.5-year-old seedlings and rooted cuttings of R. ‘Cunningham’s White’ and R. ‘Catawbiense Grandiflorum’. Their reactions depended mostly on calcium salt type added to the substrate (sulphate or carbonate). An increase in concentrations of phenolic compounds was detected mostly in roots of the plants grown in a substrate with a high calcium carbonate content. Addition of calcium salts to the substrate caused a significant rise in total nonstructural carbohydrates in leaves and roots of the studied plants. As compared to the control, an increase in substrate pH in the variant with calcium carbonate limited the activity of acid phosphatase, while lowering of substrate pH in the variant with calcium sulphate, significantly increased its activity. Along with the rise in substrate pH, a remarkable increase was observed in the activity of nonspecific dehydrogenase (DHA) in the substrate with CaCO3, as compared to the control. Unfavourable soil conditions (high calcium content and alkaline pH) caused a decrease in assimilation of minerals by the studied plants (mostly phosphorus and manganese). Our results show that the major factor limiting rhododendron growth is an increase in substrate pH, rather than an increase in the concentration of calcium ions.
High concentration of heavy metals in organic soils may be the result of intensive, deep-seated fires causing high temperatures. This research has been carried in four postfire areas, located in forests or on meadows in Lower Silesia. The aim has been to determine the impact of some parameters on the content of heavy metals in soils under postfire meadows and forests. The concentration and pool of the analyzed heavy metals were determined in soils against the background of such parameters as the depth in a horizon, organic matter content, soil colour and soil reaction. Twenty Histosol soil profiles (85 soil samples) were analyzed, representing peat-muck, muck (MtIIc1, MtIIIc1) and mineral-mucky (Me11) and on-muck soils (according to the Polish taxonomy of soils). The soils were strongly desiccated. Some physicochemical and chemical properties of pyrogenic soils were analyzed in dry samples collected into plastic bags with an Instorfu auger. Heavy metals (Zn, Cu, Pb, Ni, Cr) were determined in HCl + HNO3. Pools of heavy metals were recalculated into g m–2 in 0-20 cm layers of soils and compared among forest and meadow soils. Due to intensive fires, the reaction of pyrogenic soils was slightly acid, neutral or alkaline. As a consequence of the high temperatures, a broad spectrum of soil colours was noticed. The results showed increased concentrations of heavy metals in the topmost and muddy soil horizons. The alkaline soil reaction favoured accumulation of heavy metals. Pools of heavy metals in soils were significantly lower in forest than in meadow areas, which could be attributed to different time periods which elapsed since the fires. The Hue tint of the colour of soil horizons containing ashes indicated the presence of oxidized iron forms and a low content of organic carbon. The accumulation of heavy metals in post-fire sites in Lower Silesia was negatively correlated with the soil’s strongly acid reaction. The determined concentrations of heavy metals did not exceed the threshold values set for unpolluted soils
High content of calcium compounds in soil limits cultivation possibilities of the majority of cultivars of rhododendron. In the research presented an attempt was made to determine the influence of a high level of calcium salts CaCl2, CaSO4, Ca(NO3)2 and CaCO3, pH and an increased level of auxins in a medium, on the development of microcuttings of R. ‘Catawbiense Grandiflorum'. On the basis of the results of the research it is justifiable to state that it was the anions of some salts used that had an adverse effect on the development of rhododendron cultures, not calcium cations. Significant differences were noted in the uptake of calcium, magnesium and sodium ions by the microcuttings depending on salts used in the media. The pH value of the medium higher than optimum significantly affected the degree of chlorosis of the microcuttings analyzed. Supplementing the media with additional auxin - IBA was stimulating for the plant growth especially in the medium containing CaCO3.
The aim of our paper was to determine selected morphometric indices of the main fabric units of humus layers in soils with different vegetation cover and contaminated by heavy metals. The study sampled soil from three sites located about 4 km NW from a zinc plant. In all humus layers decomposed organic matter occurred mainly in form of fine excrements, originated from small soil arthropods, while macrofauna excrements were the absent. The dominance of soil mesofauna over macrofauna was probably attributed to elevated heavy metal concentrations. The high concentration of heavy metals affected the major soil components, but the kind of vegetation and the degree of cover of the soil surface were as important for the development of the humus layers as heavy metal contamination.
In the present study we have investigated the effect of exogenous cyclitols on accumulation of their galactosides and raffinose family oligosaccharides (RFOs) in maturing smooth tare (Vicia tetrasperma [L.] Schreb) seeds. Feeding D-pinitol to pods of smooth tare increased the amount of free D-pinitol and its galactosides: galactopinitol A, galactopinitol B, di- and trigalactopinitol A in seeds. Similarly, feeding D-chiro-inositol, which does not occur naturally in Vicia seeds, resulted in the transport of this cyclitol in the seed, and caused accumulation of high levels of D-chiro-inositol galactosides (fagopyritol B1, B2 and B3). Accumulation of both cyclitols and their galactosides drastically reduced accumulation of verbascose and, to a lesser extent, stachyose and di-galactosyl- myo-inositol. Feeding D-chiro-inositol also decreased accumulation of di- and tri-galactosyl pinitols, naturally occurring in seeds. Inhibition of RFOs accumulation by elevated levels of free cyclitols indicates competition between biosynthesis of both types galactosides, and similarity of both biosynthetic pathways in smooth tare seeds.
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