Wyniki wyszukiwania

1

Comparative studies of sugar and protein kinases: genetic relationships and consensus sequences

100%

Zdyb A., Leluk J.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2A

2

Further studies on the role of phospholipids in determining the characteristics of mitochondrial binding sites for type I hexokinase

100%

Hutny J., Wilson J. E.

Acta Biochimica Polonica

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2000

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tom 47

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nr 4

Previous work has indicated that two types (A and B) of binding sites for hexokinase exist, but in different proportions, on brain mitochondria from various species. Hexokinase is readily solubilized from Type A sites by glucose 6-phosphate (Glc-6-P), while hexokinase bound to Type B sites remains bound even in the presence of Glc-6-P. Type A:Type B ratios are approximately 90:10, 60:40, 40:60, and 20:80 for brain mitochondria from rat, rabbit, bovine and human brain, respectively. The present study has indicated that MgCl2-dependent partitioning of mitochondrially bound hexokinase into a hydrophobic (Triton X-114) phase is generally correlated with the proportion of Type B sites. This partitioning behavior is sensitive to phospholipase C, implying that the factor(s) responsible for conferring hydrophobic character is(are) phospholipid(s). Substantial differences were also seen in the resistance of hexokinase, bound to brain mitochondria from various species, to solubilization by Triton X-100, Triton X-114, or digitonin. This resistance increased with proportion of Type B sites. Enrichment of bovine brain mitochondria in acidic phospholipids (phosphatidylserine or phosphatidylinositol), but not phosphatidylcholine or phosphatidylethanolamine, substantially increased solubilization of the enzyme after incubation at 37°C. Collectively, the results imply that the Type A and Type B sites are located in membrane domains of different lipid composition, the Type A sites being in domains enriched in acidic phospholipids which lead to greater susceptibility to solubilisation by Glc-6-P.

3

Some enzymes of the starch degradation pathway in rumen protozoa with special reference to Ophryoscolex caudatus

84%

Gyulai F., Coleman G. S.

Acta Protozoologica

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1991

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tom 30

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nr 1

4

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Influence of glycolysis process on the formation of meat quality parameters

84%

Wozniak K., Korpal A., Terman A.

Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica

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2017

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tom 42

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nr 2(334)

5

The function of complexes between the outer mitochondrial membrane pore [VDAC] and the adenine nucleotide translocase in regulation of energy metabolism and apoptosis

84%

Vyssokikh M. Y., Brdiczka D.

Acta Biochimica Polonica

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2003

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tom 50

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nr 2

The outer mitochondrial membrane pore (VDAC) changes its structure either volt- age-dependently in artificial membranes or physiologically by interaction with the adenine nucleotide translocase (ANT) in the c-conformation. This interaction creates contact sites and leads in addition to a specific organisation of cytochrome c in the VDAC-ANT complexes. The VDAC structure that is specific for contact sites generates a signal at the surface for several proteins in the cytosol to bind with high capacity, such as hexokinase, glycerol kinase and Bax. If the VDAC binding site is not occupied by hexokinase, the VDAC-ANT complex has two critical qualities: firstly, Bax gets access to cytochrome c and secondly the ANT is set in its c-conformation that easily changes conformation into an unspecific channel (uniporter) causing permeability transition. Activity of bound hexokinase protects against both, it hinders Bax binding and employs the ANT as anti-porter. The octamer of mitochondrial creatine kinase binds to VDAC from the inner surface of the outer membrane. This firstly restrains interaction between VDAC and ANT and secondly changes the VDAC structure into low affinity for hexokinase and Bax. Cytochrome c in the creatine kinase complex will be differently organised, not accessible to Bax and the ANT is run as anti-porter by the active creatine kinase octamer. However, when, for example, free radicals cause dissociation of the octamer, VDAC interacts with the ANT with the same results as described above: Bax-dependent cytochrome c release and risk of permeability transition pore opening.

6

Some specific and non-specific phosphatases in the sporocyst of Fasciola hepatica. 3. Enzymes associated with glycolysis

84%

Humiczewska M.

Wiadomości Parazytologiczne

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1997

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tom 43

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nr 4

Using histochemical and cytophotometric methods, enzymes associated with anaerobie glycolysis: glucose-6-phosphatase, hexokinase, and aldolase in the developing sporocysts of Fasciola hepatica were studied. Highest activity of these enzymes was found in the germ balls in the sporocysts, at all phases of their development, which is related to intensive proliferation and differentiation of the embryos developing inside the germ balls.

7

Sucrose uptake and transport are inhibited by okadaic acid during regeneration of the sugar-starved Vicia faba root meristem cells

67%

Polit J. T., Zabka A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

8

Substrate specificity of glucokinase and fructokinase of several conifer species

67%

Bergmann F., Mejnartowicz L.

Acta Societatis Botanicorum Poloniae

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2002

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tom 71

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nr 2

9

Effect of high-protein diet on glycolytic processes in skeletal muscles of exercising rats

51%

Luczak-Szczurek A., Flisinska-Bojanowska A.

Journal of Physiology and Pharmacology

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1997

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tom 48

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nr 1

10

Acclimation of poplar trees to heavy metals in polluted habitats: I. Carbohydrate metabolism in fine roots of Populus deltoides

51%

Lorenc-Plucinska G., Stobrawa K.

Acta Societatis Botanicorum Poloniae

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2005

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tom 74

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nr 1

Concentrations of total nonstructural carbohydrates (TNC), soluble carbohydrates, starch, sucrose, glucose, fructose, raffinose, galactose, stachyose, mannitol and specific activities of soluble acid (AI) and neutral (NI) invertases, sucrose synthase (SuSy), hexokinase (HK), fructokinase (FK), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glucose 6-phosphate dehydrogenase (G6PDH) were analyzed in fine roots of Populus deltoides Bartr. ex Marsh growing at a polluted site (near copper smelters) and a control site (free from heavy pollution). Also chemical properties of the soil from both sites were assessed. In comparison with the control, fine roots from the polluted site contained greater concentrations of TNC, soluble sugars, starch and sucrose but less hexoses, so they had higher values of sucrolysis index (sucrose/hexoses). The activity of AI, NI and SuSy declined insignificantly, while specific activities of HK, FK, GAPDH and G6PDH were significantly inhibited. The results suggest that a long-term heavy metal stress leads to an accumulation of carbohydrates and altering activities of glycolysis and the oxidative pentose phosphate pathway in fine roots.

Ograniczanie wyników

Comparative studies of sugar and protein kinases: genetic relationships and consensus sequences

Further studies on the role of phospholipids in determining the characteristics of mitochondrial binding sites for type I hexokinase

Some enzymes of the starch degradation pathway in rumen protozoa with special reference to Ophryoscolex caudatus

Influence of glycolysis process on the formation of meat quality parameters

The function of complexes between the outer mitochondrial membrane pore [VDAC] and the adenine nucleotide translocase in regulation of energy metabolism and apoptosis

Some specific and non-specific phosphatases in the sporocyst of Fasciola hepatica. 3. Enzymes associated with glycolysis

Sucrose uptake and transport are inhibited by okadaic acid during regeneration of the sugar-starved Vicia faba root meristem cells

Substrate specificity of glucokinase and fructokinase of several conifer species

Effect of high-protein diet on glycolytic processes in skeletal muscles of exercising rats

Acclimation of poplar trees to heavy metals in polluted habitats: I. Carbohydrate metabolism in fine roots of Populus deltoides