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Allene oxide synthase (AOS, EC 4.2.1.92) is the first specific jasmonate biosynthetic pathway gene. In this study, a full-length cDNA of AOS gene was cloned from common wheat nannong 9918. The gene contains an open reading frame (1,446 bp) encoding 418 amino acids. Comparative and bioinformatic analysis revealed that the deduced protein of TaAOS was highly homologous to AOSs from other plant species. The transcript of TaAOS was found to be abundantly expressed in the flag leaves, and was up-regulated by the inoculation of B. Graminis (DC.) E.O. Speer f. sp. Tritici (Bgt) in wheat leaves. In addition, it was also induced by high concentration of NaCl and ZnCl₂. When TaAOS was overexpressed in tobacco leaves via Agrobacterium tumefaciens infection, the transgenic tobacco plants displayed stronger tolerance to ZnCl₂ stress compared to transgenic GFP plants. Taken together, the above facts demonstrated that TaAOS may play a role in response to diverse stresses in plants.
Two cytotypes of Chenopodium album, diploid (2n=2x=18) and hexaploid (2n=6x=54), were analysed using flow cytometry and a FISH experiment. The genome size was indicated as 1.795 pg for the diploid and 3.845 pg for the hexaploid plants which suggested genome downsizing in the evolution of hexaploid cytotype. Double FISH with 25S rDNA and 5S rDNA allowed three to five homologue chromosome pairs to be distinguished depending on the cytotype. The Variation in size and number of rDNA sites between the polyploid C. album and its putative diploid ancestor indicated that rDNA loci underwent rearrangements after polyploidization. Flow cytometry measurements of the relative nuclear DNA content in the somatic tissue of C. album revealed extensive endopolyploidization resulting in tissues comprising a mixture of cells with a different DNA content (from 2C to 32C) in varying proportions. The pattern of endopolyploidy was characteristic for the developmental stage of the plant and for the individual organ. Polysomaty was not observed in the embryo tissues however endopolyploidization had taken place in most tested organs of seedlings. The endopolyploidy in diploid and hexaploid C. album was compared to find any relationship between the pattern of polysomaty and polyploidy level in this species. This revealed that polyploid plants showed a decline in the number of endocycles as well as in the frequency of endopolyploidy cells compared to diploid plants.
DNA markers are used not only to estimate genetic similarity and distance but also to select and identify desirable forms, to assess the adjustment of breeding material, to confirm crossbreeding efficiency, to determine seed purity, and to identify the genes which determine important functional traits. In the case of oat, DNA markers were used to construct and increase the density of genetic maps both in hexaploid and diploid species. The development of markers for some important traits provides a fast selection of genotypes containing dwarf genes as well as the resistance genes to crown rust and powdery mildew. Numerous analyses of genetic similarity between different species belonging to the genus Avena which are currently carried out may contribute to explaining the process of evolution within this genus and may also explain the development of particular species of oat.
The aim of presented paper was to assess suitability of SSR markers developed in wheat in order to evaluate genetic similarity of hexaploid species of Avena genus. Three A. sativa cultivars, 3 A. sterilis and 2 A. fatua accessions were analysed. Out of 55 tested microsatellite primer pairs: wms, wmc and gdm 12 were chosen. Based on microsatellite loci diversity genetic similarity was calculated and cluster analysis of NJ (neighbour joining) method was conducted. A dendrogram thopology indicated that A. fatua clustered closer to A. sativa than did A. sterilis. On the basis of conducted research it can be stated that there is a possibility of utilizing wheat microsatellie markers in the analysis of DNA polymorphism of hexapliod species of the Avena genus.
Somatic chromosome number was investigated in twenty doubled haploid (DH) lines derived from anther culture of two triticale cultivars. Nine lines have 42 chromosomes, the rest ranging from 39 to 43. The majority of aberrants (90%) are hypoaneuploids (2n = 39 to 41), but within the KR424 line both hypo- (2n = 39 to 41) and hyperaneuploid (2n = 43) chromosome numbers were identified. The observed frequency of seedlings with aneuploid chromosome numbers within several unstable lines varied from 4% to 31.2%.
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