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In arid and semi-arid ecosystems, shrubs have an important effect on neighboring plants. However, little is known about the interaction of herb growth stages and shrub location on herb performance. We selected Reaumuria soongorica, (Pall.) Maxim a shrub dominant in the semiarid region of northwest China, to determine whether (1) shrubs facilitate or have negative effects on neighbouring herbaceous vegetation, and (2) such effects vary with herb growth stage and with shrub orientation relative to herbs. The presence of herbaceous plant species, plant density, plant height, and percent cover were determined along 2 m long transects spreading in four directions from the base of shrub – east (transect E), west (transect W), south (transect S), and north (transect N); this was repeated for three growth stages (in May, June and July). Results indicated that the effects of R. soongorica on neighboring herbs in different growth stages were similar. Species number of herb-layer plants tended to increase from beneath the canopy to the opening, but plant density, cover and plant height decreased with distance away from shrub base. The presence of R. soongorica had positive effects on density, cover, and plant height, and negative on the number of herbaceous species during the entire growing season. Herbaceous plants growing on transect N under the shrub canopy had significantly higher density and percent cover than those growing in other directions. Biomass of herbs on transect N grown under the shrub canopy was higher than that of herbs on other transects. We concluded that shrub effects on neighbouring herbaceous vegetation were closely related to the shrub orientation relative to the herbs. Therefore, using shrubs as nurse plants for grass-growing must consider the relative placement of shrubs.
Aquatic ecosystems are receivers for various pollutants, for instance, Chromium (Cr, one of the toxic heavy metals) and phosphorus (one of the biggest causes of water eutrophication). Such contaminants have brought serious impact on health and security of aquatic ecosystems. Physiological integration between the integrated ramets of clonal plants can increase their tolerance to environmental stress. It is hypothesized that physiological integration and phosphorus could facilitate the expansion of amphibious clonal plants from soil to Cr-polluted aquatic habitats. This study was conducted to primarily examine how physiological integration and phosphorus affected the effects of Cr on plant growth and population expansion. An amphibious clonal herbaceous plant Alternanthera philoxeroides was used to simulate the spread process by induced stolon connection or disconnection, Cr pollution and different levels of phosphorus in aquatic habitats. We found that Cr pollution deployed to apical ramets directly decreased the growth and photosynthetic parameters of the apical ramets that lacked connections to the basal ramets, but these effects could be mitigated by stolon connections. Cr pollution had no effects on these disconnected basal ramets, but the transmission of Cr from the apical parts via connected stolons negatively affected the growth of the basal ramets. Increasing the phosphorus not only increased the growth but also decreased the levels of Cr that accumulated in the plant tissues and the bioactivity of Cr. These results indicated that increasing the phosphorus could benefit the population expansion and establishment of A. philoxeroides through physiological integration from terrestrial to Cr-polluted aquatic habitats.
The genome doubling agent colchicine was used effectively to obtain tetraploid plants in dragonhead. Treatment of apical meristem of seedlings was carried out in two stages. The first stage was when the cotyledon leaves emerged. The second stage was when the two true leaves emerged. Six levels of colchicine concentrations: 0, 0.05, 0.1, 0.2, 0.5, and 0.75% were applied in each of these stages. Seedling treatment in the stage of emergence of two true leaves with 0.1% colchicine solution proved to be the most effective in producing autotetraploids. Morphological, cytological and flow cytometry analyses showed the increase of chromosome numbers from 2n=2x=10 to 2n=4x=20. The increase of ploidy levels caused major changes in some morphological and physiological traits and active substances in dragonhead.
The pollen of mugwort, plantain, sorrel, nettle and pigweed is an important airborne allergen source worldwide. The occurrence of pollen grains in the air is a seasonal phenomenon and estimation of seasonal variability in the pollen count permits evaluation of the threat posed by allergens over a given area. The aim of the study was to analyse the dynamics of Artemisia, Plantago, Rumex, Urticaceae and Chenopodiaceae pollen season in Szczecin (western Poland) in 2004–2008 and to establish a relationship between the meteorological parameters versus the pollen count of the taxa studied. Measurements were performed by the Hirst volumetric trap (model Lanzoni VPPS 2000). Consecutive phases during the pollen season were defined for each taxon (1, 5, 25, 50, 75, 95, 99% of annual total) and duration of the season was determined using the 98% method. On the basis of this analysis, temporary differences in the dynamics of the seasons were most evident for Artemisia. Correlation analysis with weather parameters demonstrated that the maximum wind speed, mean and maximum air temperature, relative humidity and dew point are the main factors influencing the average daily pollen concentrations in the atmosphere.
We studied the phylogeography of Swertia tetraptera Maxim, which is an annual herbaceous plant endemic to the Qinghai-Tibetan Plateau (QTP), by sequencing one intergenic chloroplast spacer, trnL-trnF (773 bp). The sampling design included 35 populations and 399 individuals, and spanned the entire distribution of the species. Forty-four haplotypes were characterized, and one of them was widely distributed in all of the populations. The level of differentiation among the populations studied was relatively low (GST = 0.128). Estimates of NST -GST for populations of S. tetraptera indicated that no phylogeographical structure exists, which was supported by the distribution of haplotypes. The neutrality test, mismatch distribution and a ‘star-like’ genealogy all suggested that this species experienced population expansion. According to the number of rare haplotype and geological evidence, this study suggested that two potential refugia existed during the last glaciation: the first refugium was identified in a restricted semi-continuous area around the eastern margin of the plateau; the second refugium was located in the central of QTP. In fact, the findings of our study are somewhat similar as the third phylogeographical structure occurring in the QTP, that is, alpine plants have refugia not only in the edge area but also in the Plateau platform. However, the location of plateau edge and plateau platform refugia is very different among them due to the difference of species-specific characteristic such as distributional range and life history traits.
Studies on diversity of fungi colonizing and damaging different plant parts of oregano (Origanum vulgare L.) were carried out in 2012–2014 on production plantations grouped in south-eastern Poland. Fungi were isolated from superficially disinfected roots, stems and leaves using mineral medium. Fungi from Fusarium spp., Boeremia exigua var. exigua and Rhizoctonia solani were obtained from the roots and stems showing necrosis and tissue disintegration. Alternaria alternata and Stemphylium botryosum were isolated from the leaves with symptoms of irregular, necrotic spots. Colletotrichum fuscum was commonly obtained from the leaves showing symptoms necrotic, concentrically zoned spots with lighter center and the slightly raised edge. This species had not been found in Poland earlier.
This research regards the forests of Krynica surroundings (Western Carpathians). The object of the studies is to analyze the vegetal cover species in the undergrowth of deciduous mountain forest and mixed mountain forest, and to find floristic differences between these two types of forest environment. This subject was taken up because species indicating the type of environment in the Carpathian Region are useless in identifying the type of forest environments in Gorce and Beskid Sądecki Mountains. In the research, data from 50 model surfaces was used. In the studies the diagnostic value of indicator species, up to now used for forest environment identification, was judged according to their response for change in soil generosity of upper soil horizons. The types of soil found on model surfaces were divided into four groups, characterized by similar chemism of upper soil horizons (Fig. 1). Chemism of soil types in those groups is presented in Figure 2. The frequency of occurrence of different herb species depending on soil generosity is shown in Table 2. Specics of plants growing on soil of lesser generosity and receding as soil generosity increases, and those which became more frequent as the soil generosity increases, are useful to identify deciduous mountain forest and mixed mountain forest environments. Other species глге nearly useless for environment identification. It was also discovered, that species which became more frequent as soil generosity increases are joined by a significant correlation of coexistence (Fig. 3). Last phase of research defines forms of vegetal cover in forest undergrowth useful for differentiating between the two types of forest environment. Such forms are described by occurence of groups of plant species (lab. 3). It was found that the mixed mountain forest, growing on soil with upper horizons of low generosity, differs from the deciduous mountain forest by occurrence of plant species of Vaccinium group and lack, of Mercurialis group species. Typical mixed mountain forest, where upper soil horizons are of middle generosity, is similar to deciduous mountain forest because of presence of Mercurialis group species, but differs by occurrence of Vaccinium group species. The mixed mountain forest with upper soil horizons of high generosity docs not differ by floristic composition from the deciduous mountain forest. Soil generosity and productivity of both environments is caused by lower soil horizons.
Na podstawie wykonanych doświadczeń szklarniowych ustalono, że grzyby F. culmorum i F. oxysporum są chorobotwórcze dla korzeni bulwiastych oraz sadzonek zielnych dalii. Patogeniczność ich zależy w dużym stopniu od odległości od wegetatywnie mnożonego materiału roślinnego. W badanych warunkach większą chorobotwórczość wykazał F. culmorum.
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