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11 polysaccharide fractions were isolated from the Echinacea purpurea herb (collected from the cultivation) and 8 compounds were isolated from the callus tissue from in vitro cultures. The HPLC analysis of hydrolysed polysaccharides indicated a great heterogeneity of the herb fractions. They differ significantly from the compounds obtained from the callus tissue, which are mostly arabinogalactans with an addition of xylose.
Phenolic acids from herb of Linaria vulgaris (L.) Mill, were analysed by means of two di­mensional thin layer chromatography and RP-HPLC method. Ten acids were identified: protocatechuic, gallic, caffeic, p-hydroxybenzoic, p-coumaric, vanillic, ferulic, homoprotocatechuic, salicylic and o-hydroxyphenylacetic. By the RP-HPLC method the content of five major compounds in fraction of free phenolic acids was determined.
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The content of dry matter, total protein, ether extract, crude ash and crude fibre was determined in the plant material. In addition, the BAW was calculated and the content of: Mg, Ca, K, Na, Fe, Cu, Mn and Zn was assessed. The highest (P < 0.05) concentration of nutrients in dry matter was characteristic of fennel flower and green pepper.The highest abundance of minerals in crude ash was found in lovage and marjoram (144.4-116.6 g kg-1), and of total protein – in common basil and lovage (208.8 and 185.4 g kg-1). The highest (P < 0.05) content of crude fat was determined in common juniper, nutmeg, green pepper, marjoram and rosemary (on average 17.18 g kg-1) and of fibre in coriander (107.2 g kg-1), while the highest BAW was recorded in nutmeg, white pepper and rosemary (on average 730.9 g kg-1). The % RDA and AI coverage were estimated for consumers aged 31-50, assuming that the intake of the analysed culinary herbs is equivalent to 1g per day. The herbs had highly differentiated content of the analysed minerals. Common basil contained the highest amounts (P < 0.05) of macroelements: Mg, Ca, K and Na (79.8, 1278, 2135 and 218.5 μg g-1, respectively) and microelements: Fe, Cu and Mn (26.31, 1.95 and 8.56 μg g-1, respectively). Of all the herbs, fennel flower was the most abundant (P < 0.05) source of Zn (74.53 μg g-1), while juniper and green pepper was the richest in Mg (an average content 86.8 μg g-1), marjoram and lovage – in Ca (1666 and 1041 μg g-1 respectively), and red pepper – in K (2114 μg g-1). According to calculations, the consumption of 1 g of the above herbs can cover up to approx. 0.1% of RDA (Mg, Ca,) and AI (K, Na) and up to 0.6% of RDA (Fe, Cu, Mn, Zn).
A comparative study of the sterol components of roots and herb of H. pilosella was carried out using GCMS techniques, β-sitosterol, stigmasterol, campesterol and α-saccharostenon were identified in both fractions. Stigmast-7-en-3-ol was identified only in herb and stigmast-4-en-3-on only in roots. Sitosterol and stigmasterol were the predominant sterol components in analysed raw materials. Besides, by means of the weight method the total amounts of sterols was determined: in herb 0.24% and in roots 0.16%.
Herbs and spices are commonly used in cuisine all over the world. They may contain many various substances beneficial for health, but also harmful (e.g. metallic) elements. Spices and herbal plants contain metal ions over a wide range of concentrations. Metals can have important positive or negative roles in human life. The aim of this study was to estimate the trace metals content in select popular Polish herbs and spices available on the Polish market. Thirty samples of various herbs and spices (fennel, sage, oak bark, St. John's wort, linden inflorescence, mint, black pepper, garlic, marjoram, paprika, cinnamon, basil, oregano, herbs de Provence, and parsley) were analyzed. The contents of Cu, Ni, Fe, Zn, and Mn were determined using AAS method after sample mineralization with 65% nitric acid and 30% hydrogen peroxide. Metals contents were compared in spices from different manufacturers. The chemometric techniques were used to evaluate similarity with respect to herb and spice metal contents.
The present study was aimed to develop a cost-effective and efficient protocol for mass propagation of high-quality seedlings through tissue culture by using seaweed extract as biostimulants instead of synthetic chemicals. The nodal explant of field grown W. somnifera estabilised on Murashige and Skoogs medium (MS) and Gamborg B5 medium supplemented with six concentration of 2,4 D. The percentage of culture response from the nodal explant ranged from 44 to 80 and 3.0 mg l-1 2,4 D found to be best for callus induction. MS media containing different concentration of seaweed extract (10, 20, 40, 60, 80 and 100 %) were tested individually for shoot induction. The medium supplemented with 40 % seaweed extract exhibited maximum number of shoots with about 8.6 shoots/ callus and 80 % seaweed extract exhibited 4.3 shoots/ callus. It is evident from this study that seaweed extracts can be used as substitute for synthetic growth hormones for micropropagation of medicinally important plant W. somnifera for clonal propagation and conservation.
P. exigua var. exigua was isolated from underground and aboveground organs of different herb plant species in the years 1998-2006. Pathogenicity tests of three randomly chosen isolates of the fungus T 299, T 261 and T 277 for thyme and of three isolates M 1657, M 1193 and M 1635 for lemon balm were carried out. The effect of water suspension of fungus spores on the germination ability of schizocarps and of infected soil on sprouting and seedling health was studied. The study of microscopic structures of the fungus developing on plants, isolation of the fungus on artifi cial media and macro- and microscopic analysis on standard media are essential for the correct identifi cation of P. exigua var. exigua. The polyphagous nature of the fungus is confi rmed by the obtained results. The tested isolates were found to be occasional pathogens of thyme and lemon balm in the studied conditions. The harmfulness of the tested isolates pointed to inhibition of schizocarp germination, sprouting of plants and the ability to cause necrosis of germs, roots and epicotyl.
Drying is the most common and also the simplest method of preserving fresh plant materials. This process greatly extends the life of the product by the removal of water, which decreases the rate of chemical and enzymatic reactions, or even inhibits them. The drying step can cause a change in appearance, taste, color and consistency, as well as reduce the quantity of essential oils, polyphenols, pigments and vitamins. These changes can be significantly reduced by using suitable drying techniques, depending on the material. The choice of the technique should be based on knowledge of the biological, physical and chemical characteristics of the raw material. The paper presents the effects of drying on the quantitative loss of essential oils and compounds with antioxidant properties, as well as changes in the antioxidant properties of selected herbs and spices dried by different methods. Both, the food industry and the cosmetics or pharmaceuticals industry generates a demand for high-quality raw materials, and hence research is being conducted both on preservation methods and on their impact on the quality of the obtained material. It seems that the new technology of drying material in a fluidized bed, greatly reduces or even eliminates the disadvantages of current procedures.
The aim of this work was an assessment of a microbiological quality of some dried herbs, offered in retail. A basil (Ocimum basilicum L.), marjoram (Origanum majorana L.), oregano (Origanum vulgare L.) savory (Satureja hortensis L.), tarragon (Artemisia dracunculus L.) and thyme (Thymus vulgaris L.) were investigated. The research material included 28 samples coming from five producers and was purchased in Szczecin in shops of various commercial networks. It was stated that the microbiological contamination was significantly differentiated depending on both: a kind of herbs and a producer. A mean count of microorganisms was the highest in basil while the lowest counts were detected in oregano and savory. A microbiological quality of the majority of the tested herbs attained standards. However, in 25% of the samples an excessive amount of bacteria (>105cfu∙g-1) was detected. Only in individual cases, there was a high contamination by moulds (>103cfu∙g-1) and coliforms (present in 0.01–0.001 g). Nevertheless, there were no E. coli, Salmonella sp. and coagulase (+) staphylococci in any sample.
In this work the phenolic acids in the methanol extract from the flowering herbs of Cirsium vulgare (Savi) Ten. growing in Poland were isolated and identified. The samples containing free phenolic acids and those released after acid and alkaline hydrolyses were investigated by 2D TLC on cellulose. After purification by SPE, samples were also analyzed by RP-HPLC. Six phenolic acids such as gallic, protokatechuic, gentisic, hydrobenzoic, vanillic and caffeic acids were detected in the fraction of free phenolic acids of the methanol extract, irrespectively of the method used.
This study recorded and analyzed traditional knowledge of medicinal plants in the Turkestan Range in southwestern Kyrgyzstan, where ethnobotanical knowledge has been largely under-documented to date. Data was collected through participant observation and both semi-structured and in-depth interviews with 10 herbal specialists. A total of 50 medicinal plant taxa were documented, distributed among 46 genera and 27 botanical families. In folk medicine they are applied in 75 different formulations, which cure 63 human and three animal ailments. Quantitative ethnobotanical indices were calculated to analyze traditional knowledge of the informants and to determine the cultural importance of particular medicinal plants. Ziziphora pamiroalaica, Peganum harmala, and Inula orientalis obtained the highest use value (UV). The best-represented and culturally important families were Lamiaceae, Asteraceae, and Apiaceae. Gastro-intestinal system disorders was the most prevalent ailment category. Most medicinal plants were gathered from nearby environments, however, species with a higher cultural value occurred at distant rather than nearby collection sites. The findings of this study proved the gap in documentation of traditional knowledge in Kyrgyzstan, indicating that further studies on the traditional use of wild plant resources could bring important insights into ecosystems’ diversity with implications to human ecology and biocultural diversity conservation in Central Asia.
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The aim of the investigation carried out in 2006-2007 was to assess differences between 5 grass species and 5 species of plants belonging to meadow herbs in their ability to accumulate selenium. According to the results, among the grass species, the samples of Arrhenatherum elatuis L. had the highest average Se content (46 μg Se⋅kg-1 d.m.), and the lowest average Se amount was found in Holcus lanatus L. (24 μg Se⋅kg-1 d.m.). The content of Se in meadow weeds (70 μg Se⋅kg-1 d.m.) was approximately twice as high as in grasses (32 μg Se⋅kg-1 dm.). Among this group of plants, the highest Se accumulation occurred in Equisetum arvense L. (103 μg Se⋅kg-1 dm.) and the lowest – in Taraxacum officinale (59 μg Se⋅kg-1 dm.). Both in grasses and in meadow weeds, the Se content significantly depended on the amount of this element in soils. In the following species: Dactylis glomerata L., Arrhenatherum elatius L., Poa pratensis L. and Taraxacum officinale, the amount of accumulated Se was also conditioned by the soil’s abundance in organic and total C.
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