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1

Autophagy, apoptosis, mitoptosis and necrosis: interdependence between those pathways and effects on cancer

100%
Chaabane W., User S. D., El-Gazzah M., Jaksik R., Sajjadi E., Rzeszowska-Wolny J., Los M. J.
Archivum Immunologiae et Therapiae Experimentalis
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2013
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tom 61
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nr 1
2
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Only live Helicobacter pylori is capable of caspase-3 dependent apoptosis induction in gastric mucosa epithelial cells

100%
Pierzchalski P., Pytko-Polonczyk J., Jaworek J., Konturek S. J., Gonciarz M.
Journal of Physiology and Pharmacology
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2009
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tom 60
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nr 4
119-128
Physiological process of cell death, apoptosis, plays a beneficial role in organism survival, but in some pathologies, like gastric Helicobacter pylori (Hp) infection, this process may turn against the host organism causing tissue damage. Knowledge of the mechanisms controlling apoptosis may have potential significance in treatment of these pathologic states. Therefore, we sought to determine whether apoptosis induced in the gastric epithelial cells exposed to live Hp involves the alteration in heat shock protein 70 (HSP70) expression and activation of caspase-3 in peroxisome proliferator-activated receptors (PPARg dependent manner). Experiments were performed with KATO III, gastric epithelial cells, exposed to CagA and Vac A positive live Hp , water Hp extracts or Hp culture supernatant over different time periods. Total cellular RNA and proteins were isolated for PCR, western-blot and EMSA studies. Genomic DNA was isolated to analyze apoptosis status. We propose new model of Hp induced HSP70 dependent, caspase-3 executed apoptosis in human gastric epithelium. KATO III cells exposed to Hp , showed an increase in caspase-3 activity accompanied and preceeded by activation of nuclear translocation of PPAR peaking at 48 h of culture. Moreover, heat shock factor 1 (HSF-1) bound up with phosphorylated STAT-3 was unable to activate HSP70 protein synthesis in KATO III exposed to Hp . Lack of protective effect of HSP70, activation of caspase-3 - dependent apoptosis pathway caused by Hp and alteration of the bax/bcl-2 cellular equilibrium led to gastric epithelial cell death. The observed phenomenon might be helpful in understanding of the mechanism of Hp related gastrointestinal tract diseasess, especially gastric cancer.
3
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The dynamics of heat shock system activation in Monomac-6 cells upon Helicobacter pylori infection

100%
Pierzchalski P., Jastrzebska M., Link-Lenczowski P., Leja-Szpak A., Bonior J., Jaworek J., Okon K., Wojcik P.
Journal of Physiology and Pharmacology
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2014
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tom 65
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nr 6
4
Content available remote

Monocytes and vascular endothelial cells apoptosis. Role of p-HSP27

100%
Gawad A., Ptak-Belowska A., Brzozowski T., Pawlik W. W.
Journal of Physiology and Pharmacology
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2009
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tom 60
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nr 4
55-61
The aim of this study was to find out whether stimulated monocytes could trigger apoptosis of vascular endothelial cells. Human umbilical vein endothelial cells (HUVEC) (EC) were co-cultured for 24 h and 48 h with monocytes isolated from peripheral blood (peripheral blood monocytes) or MonoMac6 cell line activated previously with proinflammatory cytokines. Real-time PCR was conducted to investigate p53 up-regulated modulator of apoptosis (PUMA), heat shock protein HSP70 and HSP27 genes expression. Changes in the level of PUMA, HSP70, HSP27 and phospho-heat shock protein 27 (p-HSP27) proteins were analyzed by means of immunoprecipitation. Apoptosis was determined by TUNEL and poli-(ADP ribose) polymerase ( PARP ) cleavage assay. In HUVEC cells stimulated with monocytes hardly any increase of PUMA mRNA was observed, but the PUMA protein level was significantly up regulated especially after 24 h. Heat shock proteins (HSP70 and HSP27) mRNA expression was elevated after 24 h and 48h and confirmatory up regulation of these proteins was observed in HUVEC cells stimulated with peripheral blood monocytes but not with MonoMac6 cells. Interestingly, in nuclear compartment of HUVECs exposed to the monocytic line and native monocytes, a significant increase of p-HSP27 level has appeared. TUNEL and PARP cleavage assay did not show any apoptotic HUVEC cells after stimulation with monocytes. The main observations of this study indicate that monocytes do not trigger apoptosis of vascular endothelial cells. Proapoptotic activation mediated by PUMA that was observed seemed to be counterbalanced by significant increase of antiapoptotic HSP70, HSP27 and especially phospho-HSP27 proteins level.
5

Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids

100%
Kumar J., Chaudhury A., Bera B. C., Kumar R., Kumar R., Tatu U., Yadav S. C.
Acta Parasitologica
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2015
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tom 60
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nr 4
6

Location of heat shock protein 70 and metallothionein immunoreactivity associated with copper deficiency in the CNS of lambs

100%
Tunca R., Erginsoy S. D., Sozmen M., Guvenc T., Ercag E.
Medycyna Weterynaryjna
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2007
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tom 63
|
nr 10
1187-1192
The localizations of metallothionein I and II (MT), a small molecular weight heavy metal binding proteins, and 70-kDa heat shock protein (Hsp70) were investigated by immunohistochemical techniques in brains of lambs that had been injured by congenital copper deficiency. The results were compared with those obtained from control lambs. The morphological findings of the congenital copper deficiency in the central nervous system (CNS) were recorded. The amount of copper in the brain and liver of the lambs and feed of breeding ewes and soil was also assayed by atomic absorption spectrophotometry. The amount of copper in the brain, liver, soil and feed were low. Immunohistochemically, MT and Hsp70 expressions were found to be markedly increased in the CNS of congenital copper deficient lambs compared with control lambs. MT immunoreactivity was prominently found in the astrocytes while strong Hsp70 labelling was in both astrocytes and neurons in the cerebrum, cerebellum, thalamus/hypothalamus and medulla oblongata. Immunohistochemical labelling for both MT and Hsp70 was also seen in the pia mater, ependymal cells and choroid plexi. Present results suggest that the elevated expressions of MT and Hsp70 in astrocytes and neurons are possibly indicating that they are less susceptible to the consequences of cell stress factors and could be exploited to increase selectively their survival in copper deficiency.
7
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L-arginine decreases heat shock protein 70 [marker of environmental stress] expression in kidney cells of rat fetuses during apoptosis - late effect of adriamycin action

100%
Pedrycz A., Brzeski Z.
Annals of Agricultural and Environmental Medicine
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2006
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tom 13
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nr 1
Both Adriamycin and nitric oxide (NO) cause apoptosis acting through or as free radicals inciting oxidative stress in the cell. However, in some tissues the antiapoptotic action of NO was described, thereby the impact of NO on cell apoptosis is not finally recognized. In this study, a trial of the evaluation of exogenous NO (L-arginine) impact on apoptosis induced by Adriamycin in fetal kidney cells was undertaken. For this reason, the expression of Heat Shock Protein 70 (HSP 70), environmental stress marker, as a sensitive biomarker of oxidative stress induced in fetal kidney cells with Adriamycin given to mothers prior to pregnancy was studied using immunohistochemical method. The expression of HSP 70 in fetal kidney cells, whose mothers received apart from Adriamycin, L-arginine (as NO substrate) was also evaluated. The results of the study pointed to the fact that the exogenous NO (L-arginine) could be helpful in inhibition of intensified apoptosis in fetal cells as a late effect of Adriamycin action.
8

NKT-like cells reveal higher than T lymphocytes expression of cellular protective proteins HSP70 and SOD2 and comparably increased expression of SIRT1 in the oldest seniors

80%
Kaszubowska L., Foerster J., Kwiatkowski P., Schetz D.
Folia Histochemica et Cytobiologica
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2018
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tom 56
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nr 4
9

Characterization of Hsp70 proteins in bovine leukocytes induced by the temperature 41 degrees Celsius

80%
Urban-Chmiel R., Puchalski A., Wernicki A., Dec M., Paluch E.
Polish Journal of Veterinary Sciences
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2009
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tom 12
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nr 3
Heat shock proteins (Hsp) are the group of proteins observed in both prokaryotic and eukaryotic cell types. Hsp synthesis takes place in response to many environmental conditions, including ultraviolet radiation, heavy metal ions, hypoxia and toxic agents. Many authors have suggested that Hsp can be used in immunoprophylaxis, yet Hsp70 proteins expressed in bovine leukocytes have not been fully characterized. Hence the aim of this study was to evaluate the expression of Hsp70 proteins in bovine leukocytes exposed to temp. 41℃. The material for the study consisted of bovine white blood cells incubated at 41℃ for 2 hours. SDS-Page electrophoresis, Western blotting, and two-dimensional electrophoresis (2D) were performed to estimate the proteins obtained. The results of the study confirmed the influence of the temperature of 41℃ on induction of Hsp70 in bovine leukocytes. These proteins were mainly localized within molecular mass 70kDa. Some of the proteins with molecular mass from 20 to 50 kDa also showed positive reactions in Western blotting with anti-Hsp70 antibodies. Analysis of 2D electrophoresis showed a change in the localization of these proteins in the pH gradient. It can be postulated that analysis of Hsp70 expression in bovine leukocytes can be a very helpful marker for evaluating an organism’s adaptation to environmental heat stress. The proteins obtained also showed immunological reactivity with rabbit antibodies in Western blotting reactions, indicating that they can be used as protective factors in the pathogenesis of many diseases.
10
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Molecular alterations in fibroblasts exposed to Helicobacter pylori: a missing link in bacterial inflammation progressing into gastric carcinogenesis?

80%
Krzysiek-Maczka G., Targosz A., Ptak-Belowska A., Korbut E., Szczyrk U., Strzalka M., Brzozowski T.
Journal of Physiology and Pharmacology
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2013
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tom 64
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nr 1
11

Effects of the season on physiological and endocrine traits and on HSP70 in Saanen goats under Mediterranean climate conditions

80%
Yilmaz M., Altin T., Kiral F., Taskin T., Ekren Asici G. S., Kizilkaya K.
Medycyna Weterynaryjna
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2018
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tom 74
|
nr 01
This study was conducted to determine the impact of heat stress on some physiological and endocrine traits in Saanen goats raised under Mediterranean climate conditions. The effects of thermal stress on heart rate (HR), respiration rate (RR) and rectal temperature (RT) on plasma total trii-odothyronine (T3), thyroxine (T4), Cortisol (C), and HSP70 concentrations were evaluated on twenty two Saanen goats of different ages in the second week of April 2013, July 2013, October 2013 and January 2014. Climatic data such as temperature (°C) and relative humidity (%) were recorded from Spring 2013 to Winter 2014. Live body and BCS values were also recorded during this period. The physiological parameters above were measured twice on each experiment day (morning and afternoon) in all seasons. Blood samples were collected in each afternoon of the experiment day to analyze T3, T4 C, and HSP70. All data were analyzed. According to the values of rectal temperature (RT), it was estimated that the goats were under extreme heat stress only in the summer season. The heart rate (HR) values in the winter season for morning and noon periods were found statistically significant (p < 0.05). The average respiratory rate (RR) in the spring season was found significantly lower. On the other hand, the RR for the noon period in the summer was higher than in the fall and winter seasons (p < 0.05). There was a significant difference (p < 0.05) between summer and fall seasons for C values. The highest value (96.62 ng/ml) was obtained in spring, whereas the lowest (60.58 ng/ml) in the fall. T3 levels in the fall and winter were found to be statistically significant (p < 0.05). They were the highest in the winter and spring, and the lowest in fall. T4 and T4/T3 levels in the winter were found to be statistically higher than in other seasons (p < 0.05). The lowest value for T4 was found in the fall and for T4/T3 in summer. Mean HSP70 value in spring was found to be statistically low (p < 0.05). The changes in THI values in different seasons, particularly between mornings and afternoons, indicated that thermal stress was evident, and that the animals became resistant to it eventually. The fluctuations of the C, T3, T4 and HSP70 values were indicators of the animals’ reaction to thermal stress. The THI values in spring, which were between 16 and 18 THI, may be considered within the ideal comfort zone for goats. It was observed that Saanen goats were able to adapt to seasonal weather changes in the environmental conditions of the region.
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