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Background. The health benefits arising from antioxidant vitamins A, C and E are well recognised and their recommended dietary intake for the general population have been established. However, there is still a need for assessing antioxidant vitamin intake in different population groups. Objective. To assess intake of antioxidant vitamins: A, C, E and β-carotene, and to identify their major sources in the diets of healthy subjects. Material and methods. The study group consisted of 182 adults; both men and women from polish population. Antioxidant vitamin dietary intake was assessed by individual 3-day records. Data were analysed using updated “Polish Food Composition Tables” and ‘Dieta 5’ Software. Results. The average daily intake of antioxidant vitamins was: 1076 mg for vitamin A (including 46% of retinol and 55% of β-carotene), 107 mg for vitamin C and 9 mg for vitamin E. Higher dietary intake of these vitamins was observed in men compared to women. Conclusions. The average intake of antioxidant vitamins was found to be in recommended range, however, significant differences were observed between the lowest and the highest intake. They were related to differences in the consumption of food products recognized as major sources of vitamins A, C, E and β-carotene in study population.
Efficiency of L-carnitine endogenous biosynthesis in humans depends on the presence of cofactors of numerous enzymatic reactions. Among others, the role of cofactor is played by vitamin C and vitamin B6. The objective of our study was to evaluate the influence of vitamins C and B6 stores on plasma carnitine level in healthy humans. The examined group consisted of 52 healthy volunteers. Total (TC) and free (FC) carnitine, ascorbic acid (AA) in plasma and indices of vitamin B6 in plasma and in erythrocytes were determined. Mean TC was 52.2 ± 10.7 mmol/1 and FC 44.4 ± 9.5 mmol/1. Mean AA was 0.4 ± 0.3 mg/dl. In 40% of examined group AA levels were out of the reference range. B6 indices in plasma and erythrocytes were 1.21 ± 0.23 and 1.42 ± 0.33, respectively. Vitamin C and vitamin B6 did not influence carnitine plasma levels, that confirms the marginal role of endogenous biosynthesis in the carnitine balance in healthy subjects. Moreover, a significant distribution of vitamin C deficiency was found in the evaluated population.
The study was aimed to estimate changes in the use of dietary supplementation with preparations containing magnesium among adult inhabitants of Warsaw. The survey was carried out in 1994 on 396 and in 1999 on 336 persons. Respondents were randomly selected. A significant increase of the percentage of people using preparations containing magnesium was observed (from 28% to 41%). Analysis of the influence of demographic factors on the frequency of magnesium supplementation showed changes in the demographic structure of respondents using preparations with magnesium. In 1999 women used supplements more often than men; the frequency of use increased also with age and educational level. The supplements were used significantly more often by people suffering from chronic diseases. No differences between magnesium doses used in 1994 and 1999 were observed. The median dose was 67 mg per day in 1994 and 100mg per day in 1999. The percentage of persons using magnesium in doses exceeding the upper limit of intake (350 mg per day) declined significantly (from 7% to 0,7%).
A growing number of studies are being performed on the role of dendritic cells (DCs) in the etiopathogenesis of various conditions. Therefore, it is extremely important to establish the best comparable methods for the determination of the absolute count of blood dendritic cells (BDCs) or their subsets, and the reference normal values for comparisons. The aim of our study was to assess a normal profile of BDCs in the non-cultured human blood of healthy Polish volunteers. BDCs were detected among peripheral blood mononuclear cells (PBMC) from 99 healthy people, aged 18- 56. Based on the panel of novel anti-BDCA1, BDCA2 and BDCA3 monoclonal antibodies (MoAbs), three main subpopulations of BDCs were distinguished: two myeloid types of BDCs, MDC1(BDCA-1+/ CD11c+ /HLA-DR+) or MDC2 (BDCA-3+/CD32-/CD64-/HLA-DR+), and a plasmacytoid subtype, PDC (BDCA-2+/CD123+/HLA-DR+). The number and percentage of BDCs were correlated with the age, gender, photosensitivity (phototype, minimal erythemal dose - MED) and morphological parameters of the healthy volunteers. BDCs represented 0.83% of the PBMC and the median total BDC number was 44.0 cell/ml. The total BDC number correlated with the WBC count (r=0.40; p<0.001) as well as with the lymphocyte and monocyte counts (r=0.20; p=0.045 and r=0.26; p=0.009, respectively). The median percentage of the MDC1 count (0.20%) was twice as high as the MDC2 count (0.10%). The median PDC count was 28.2 cell/ml, and these cells represented 0.50% of the PBMC. There was a positive correlation between PDC and skin photosensitivity (r=0.28; p=0.005). An inverse correlation between the PDC count and the age of the examined volunteers was also found (r=-0.22; p=0.029). Our study provides the first referential data on normal rates and counts of BDCs and their subpopulations, assessed by the new panel of anti-BDCA MoAbs, in healthy Polish subjects. The method used in the study allowed the determination of BDCs and their subset numbers in a relatively small blood volume.
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