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The paracarpous gynoecium in Capsella bursa-pastoris is characterized by postgenital fusion of two carpels into a single structure representing a false two-loculated ovary. The ovular primordium is initiated by periclinal cell divisions of both the subdermal and third layers of the placenta. The ovule is ana-amphitropous, medionucellate, funicular and bitegmic, with the micropyle formed by both integuments. During development the cells of the micropylar and middle nucellar zones degenerate and the persisting chalazal zone assumes a column shape (the postamento-podium). The integuments develop according to Dermal type and Variation C (author’s term). In the mature ovule the inner integument consists of three layers, and the outer integument is two-layered except on the abaxial side of the ovule where the integuments are more massive. At the two-nucleate megagametophyte stage the inner epidermis cells of the inner integument begin to divide periclinally. These divisions are followed by differentiation, giving rise to cells that differ in their form, structure, substance accumulation and participation in seed coat organization. The inner layer, consisting of cytoplasm-rich cells with marked radial expansion, represents the endothelium. The cells of the other layer become vacuolate and extend tangentially. They form the middle layer. The cells of the outer epidermis and middle layer are destroyed (in the latter only partly) during seed development. The endothelium becomes the endotegmen (pigment layer), composed of thick-walled cells that contain tannins and possibly lipids. The outer integument gives rise to the testa, composed of an epidermal mucilaginous layer and a sclerotic (mechanical) layer consisting of cells with thickened radial and inner tangential walls and containing starch. The hypostase is differentiated at the base of the nucellus and integuments in contact with the chalaza. The vascular bundle of the ovule reaches the hypostase, which is preserved also in the mature seed and represented by 3-5 layers forming a cup. The cells of the hypostase accumulate proteins and dextrins during the late stages of ovule development, and starch after fertilization. Later, at the early globular embryo stage, the cell walls begin to lignify, the cell contents showing tannin-like substances.
The present study is concerned with auxin action in intact and excised 4th inter­node of tulip shoot after removal of the flower bud in relation to the growth stages. Elongation of the intact 4th internode with or without leaves, after removal of the flower bud, at different stages was very weak. This suggests that the flower bud is always responsible for elongation growth of the 4th internode. Auxin (IAA at 0.1%, w/w in lanolin) exogenously applied to the cut surface of tulip shoot with or without leaves, greatly stimulated the growth of the 4th and lower internodes of tulip shoot. The elongation growth of excised 4th internode of tulip shoot with or without node, after removal of the flower bud was much higher, in comparison with that of intact 4th internode in tulip shoot which was growing and in which the flower bud had been cut. Elongation depended on the initial length of the 4th internode. IAA at 0.1% applied to the cut surface of excised 4th internode, just after removal of flower bud, slightly in­creased the growth of the 4th internode. The promotion was observed only during the first or second day of the experiment. Finally, auxin did not stimulate, or had no effect on elongation of excised 4th internode with or without node. On the other hand, elon­gation growth of the excised 4 th internode with a flower bud was almost independent of the initial length of the 4th internode. Differences in the growth of excised and intact 4th internode of tulip shoot after removal of the flower bud are discussed in relation to auxin action.
This article addresses the gynoecium and embryo sac from a fresh viewpoint, that is, in terms of the apoplastic system involved in the pistil and its putative functions in sexual processes. This system includes the extracellular matrix of the pollen tube track and particularly the micropyle and synergids, the intercellular space between the cells of the female germ unit, and the apoplast surrounding the embryo sac. Most of the data cited are research results established during the last decade, mainly from ultrastructural and cytochemical observations, which give interesting and important information about the apoplastic system concerned.
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