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1

The effect of potassium channel blockers on the relaxant response of human intrauterine arteries to exogenous nitric oxide

100%
Kostrzewska A.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
2

p19 detected in the rat retina and pineal gland is a guanylyl cyclase-activating protein [GCAP]

75%
Dejda A., Matczak I., Gorczyca W. A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 4
The Ca2+ -dependent activation of retina-specific guanylyl cyclase (retGC) is medi­ated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 uM) concentrations of Ca2+ .AtlowCa2+ , immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat reti­nal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca2+ -dependent electrophoretic mobility shift.
3
Content available remote

Cyclic GMP metabolism and its role in brain physiology

63%
Domek-Lopacinska K., Strosznajder J. B.
Journal of Physiology and Pharmacology. Supplement
|
2005
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tom 56
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nr 2
15-34
Cyclic GMP (cGMP) is synthesized by guanylyl cyclase (GC) in response to nitric oxide (NO) and carbon monoxide (CO) or natiuretic peptides (NPs); atrial, brain and C-type (ANP, BNP and CNP). cGMP is degraded by several cGMP-specific phosphodiesterases (PDEs). Guanylate cyclases (GC) are differentiated into: membrane-bound/particulate (pGC) and cytosolic/soluble (sGC). In recent years evidence has accumulated that NO is the main activator of sGC and NO/cGMP plays important role in glutaminergic, cholinergic and dopaminergic signaling pathways. cGMP in the nervous system is involved in long term potentiation and depression (LTP, LTD) suggesting its participation in learning and memory mechanism. cGMP regulates calcium homeostasis and phototransduction. Its level is regulated by PDEs and their specific inhibitors protect cGMP level in cells and are very important from clinical point of view.
4
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Mechanism of action of Trolox on duodenal contractility

63%
Fagundes D. S., Grasa L., Gonzalo S., Martinez de Salinas F., Arruebo M. P., Plaza M. A., Murillo M. D.
Journal of Physiology and Pharmacology
|
2013
|
tom 64
|
nr 6
5
Content available remote

Regulation of cGMP synthesis in cultured podocytes by vasoactive hormones

63%
Lewko B., Golos M., Latawiec E., Angielski S., Stepinski J.
Journal of Physiology and Pharmacology
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2006
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tom 57
|
nr 4
The podocytes are highly differentiated cells playing a key role in glomerular filtration. Vasoactive factors including angiotensin II (Ang II) and cyclic guanosine 5' monophosphate (cGMP) are synthesized by these cells upon stimulation as well as in the basal state. In this study we have tested whether angiotensin II affects the total synthesis of cGMP in primary culture of rat podocytes. The cells were stimulated with atrial natriuretic peptide (ANP) and/or a nitric oxide (NO) donor, S-nitroso-N-acetyl penicillamine (SNAP), in the absence or presence of Ang II. The cGMP synthesis was determined by radioimmunoassay (RIA). ANP or SNAP alone increased the cGMP synthesis in podocytes although the effects were not additive unless Ang II was present in the medium. Ang II suppressed the ANP-dependent cGMP synthesis whereas SNAP-dependent cGMP production remained unaffected. These effects were prevented by a non-specific antagonist of Ang II receptors (AT), saralasin. Adversely, PD123319, a specific inhibitor of AT2 receptors, augmented inhibition of ANP-dependent and enhanced the NO-dependent cGMP production. Probenecid, an inhibitor of cGMP extrusion from the cells, suppressed the cGMP generation by both ANP and SNAP. We conclude that cGMP synthesis in cultured podocytes is modulated by angiotensin II and that two adversely acting receptors, AT1 and AT2 are involved in this effect. Additionally, production of cGMP might be intrinsically inhibited by cGMP accumulating inside the cells.
6

Metabolism of cyclic GMP in peritoneal macrophages of rat and guinea pig

63%
Kobialka M., Witwicka H., Siednienko J., Gorczyca W. A.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 3
The aim of our studies was to establish which enzymes constitute the "cGMP pathway" in rat and guinea pig peritoneal macrophages (PM). We found that in guinea pig PM synthesis of the nucleotide was significantly enhanced in response to activators of soluble guanylyl cyclase (sGC) and it was only slightly stimulated by specific activa­tors of particulate guanylyl cyclases (pGC). In contrast, rat PM responded strongly to atrial natriuretic peptide (ANP), the activator of pGC type A. The rat cells synthesized about three-fold more cGMP than an equal number of the guinea pig cells. The activity of phosphodiesterases (PDE) hydrolyzing cGMP was apparently regulated by cGMP itself in PM of both species and again it was higher in the rat cells than in those isolated from guinea pig. However, guinea pig PM revealed an activity of Ca2+ /cal- modulin-dependent PDE1, which was absent in the rat cells. Using Western blotting analysis we were unable to detect the presence of cGMP-dependent protein kinase 1 (PKG1) in PM isolated from either species. In summary, our findings indicate that particulate GC-A is the main active form of GC in the rat PM, while in guinea pig macrophages the sGC activity dominates. Since the profiles of the PDE activities in rat and guinea pig PM are also different, we conclude that the mechanisms regulating cGMP metabolism in PM are species-specific. Moreover, our results suggest that tar­gets for cGMP other than PKG1 should be present in PM of both species.
7

Ca2plus differently affects hydrophobic properties of guanylyl cyclase-activating proteins [GCAPs] and recoverin

63%
Gorczyca W. A., Kobialka M., Kuropatwa M., Kurowska E.
Acta Biochimica Polonica
|
2003
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tom 50
|
nr 2
Guanylyl cyclase-activating proteins (GCAPs) and recoverin are retina-specific Ca2+ -binding proteins involved in phototransduction. We provide here evidence that in spite of structural similarities GCAPs and recoverin differently change their overall hydrophobic properties in response to Ca2+ . Using native bovine GCAP1, GCAP2 and recoverin we show that: i) the Ca2+ -dependent binding of recoverin to Phenyl-Se- pharose is distinct from such interactions of GCAPs; ii) fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) is markedly higher at high [Ca2+ ]free (10 uM) than at low [Ca2+ ]freee(10 nM) in the presence of recoverin, while an opposing effect is observed in the presence of GCAPs; iii) fluorescence resonance energy transfer from tryptophane residues to ANS is more efficient at high [Ca2+ ]free in recoverin and at low [Ca2+ ]freee in GCAP2. Such different changes of hydrophobicity evoked by Ca2+ ap­pear to be the precondition for possible mechanisms by which GCAPs and recoverin control the activities of their target enzymes.
8

Particulate guanylyl cyclases: multiple mechanisms of activation

63%
Kobialka M., Gorczyca W. A.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 3
Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, is synthesized from GTP by a family of enzymes termed guanylyl cyclases, which are found in two forms: cytosolic (soluble) and membrane-bound (particulate). The past decade has brought significant progress in understanding the molecular mechanisms that underlie the regulation of particulate guanylyl cyclases and new members of their family have been identified. It has become more evident that the basic mechanism of catalysis of guanylyl cyclases is analogous to that recognized in adenylyl cyclases. Here we review the known basic mechanisms that contribute to the regulation of particulate guanylyl cyclases.
9
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Molecular cloning, characterization and expression of a guanylyl cyclase (HpGC1) involved in the stress signalling in Hippeastrum x hybr.

51%
Swiezawska B., Szewczuk P., Pawelek A., Jaworski K., Szmid-Jaworska A.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
|
tom 94
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nr 3
10
Content available remote

The cardiovascular effects of salidroside in the Goto-Kakizaki diabetic rat model

51%
Alameddine A., Fajloun Z., Bourreau J., Gauquelin-Koch G., Yuan M., Gauguier D., Derbre S., Ayer A., Custaud M. A., Navasiolava N.
Journal of Physiology and Pharmacology
|
2015
|
tom 66
|
nr 2
11

The cGMP synthesis and PKG1 expression in murine lymphoid organs

51%
Kurowska E., Kobialka M., Ziolo E., Strzadala L., Gorczyca W. A.
Archivum Immunologiae et Therapiae Experimentalis
|
2002
|
tom 50
|
nr 4
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