Wyniki wyszukiwania

1

Glutamate dehydrogenase [GDH] genes in yellow lupine

100%

Lehmann T., Dabert M., Ratajczak L.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

2

Molecular genotyping of Giardia duodenalis in humans in the Andimeshk county, Southwestern Iran

88%

Murrezaie E., Beiromvand M., Tavalla M., Teimoori A., Mirzavand S.

Acta Parasitologica

|

2019

|

tom 64

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nr 2

3

Glutamate dehydrogenase and glutamine synthetase activities during the development of triticale grains

88%

Kwinta J., Bartoszewicz K., Bielawski W.

Acta Physiologiae Plantarum

|

1999

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tom 21

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nr 3

4

Immunochemical comparison of the glutamate dehydrogenase isoenzymes in yellow lupin root nodules

75%

Ratajczak L., Prus-Glowacki W., Ratajczak W., Lehmann T.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

5

Glutamate dehydrogenase in higher plants

75%

Kwinta J., Bielawski W.

Acta Physiologiae Plantarum

|

1998

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tom 20

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nr 4

6

Effect of selected compounds on the activity of glutamate dehydrogenase from triticale roots

75%

Kwinta J., Bartoszewicz K., Bielawski W.

Acta Physiologiae Plantarum

|

2002

|

tom 24

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nr 3

7

The effect of Cu2plus on uptake and assimilation of ammonium by cucumber seedlings

75%

Burzynski M., Buczek J.

Acta Physiologiae Plantarum

|

1997

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tom 19

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nr 1

The ammonium uptake by cucumber seedlings was estimated from ammonium ions depletion in an uptake solution. The uptake of NH ⁺₄ was decreased by about 60 % after one hour and by about 90 % after two hours of 100 µM Cu²⁺ treatment. On the contrary the accumulation of ammonium in roots of Cu²⁺-treated seedlings at the same time was higher than in the control. Cu²⁺ in the concentration inhibiting NH ⁺₄ absorption during one hour inhibited also glutamine synthetase (GS) (EC 6.3.1.2) and NADH-glutamate dehydrogenase (NADH-GDH) (EC 1.4.1.2) activities both localized in the roots of seedlings. After one hour and at least up to the 4th hour Cu²⁺ accumulated mainly in roots (95 %). It was probably the reason of the GS activity in cotyledons of seedling treated with Cu²⁺ that it was at the same level as in the control. NADH-GDH activity in cotylcdons after one hour of the Cu²⁺ treatment was lower than in the control but the influence of Cu²⁺ action on the activity of this enzyme in roots was by far stronger. 100 µM Cu²⁺ did not affect the activities of both enzymes in in vitro experiments. Copper added into the incubation medium in 1000 µM concentration decreased GS activity, but still did not change NADH-GDH activity. These results suggested the indirect Cu²⁺ action on the investigated enzymes in in vivo experiments. However, no substantial effect on enzyme activities extracted from control plants was observed after the addition of the extract from Cu²⁺-treated plants into the incubation medium. The data suggest that the influence of Cu²⁺ on uptake and assimilation of ammonium may be connected not only with changes of plasma membrane properties in the root cells of Cu²⁺ treated seedlings but also with Cu²⁺ action on two major enzymes involved in NH ⁺₄ assimilation: glutamate synthetase and NADH-glutamate dehydrogenase.

8

Biochemical indicators of environmental contamination with heavy metals

75%

Orzechowski S., Kwinta J., Gworek B., Bielawski W.

Polish Journal of Environmental Studies

|

1997

|

tom 06

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nr 6

Changes in activity of one of the most important enzymes of nitrogen compound metabolism - glutamate dehydrogenase (GDH EC 1.4.1.2-4) under the conditions of variable concentrations of Cd2+, Zn2+, Pb2+ were investigated. Dry matter increase and glutathione (GSH) levels varying under these conditions were also analyzed. The obtained results show a close relationship between the concentration of the investigated metal ions, especially Cd2+, and the metabolism of primary amino acids. The increase of the heavy metal concentration resulted in a many-fold GDH activity increase, significant decrease of the seedling mass, and lowering of the GSH level. The increase of GDH activity may attest to the important role of that enzyme in catabolic processes intensified under these conditions, or to the increasing importance of the reductive amination of 2-oxoglutarate in the states of physiological stress caused by the presence of heavy metals. The observed relationship concerns the plant's photosynthetizing organs rather than its root system.

9

Effects of salinity stress on the activity of glutamine synthetase and glutamate dehydrogenase in triticale seedlings

75%

Kwinta J., Cal K.

Polish Journal of Environmental Studies

|

2005

|

tom 14

|

nr 1

The effect of salinity on the activity of glutamine synthetase (GS EC 6.3.1.2.) and glutamate dehydrogenase (NAD(P)H-GDH EC 1.4.1.2-4) in shoots and roots of triticale seedlings was investigated. Stressed plants were cultured hydroponically on the 100 mM NaCl-enriched medium for 7 days and the physiological responses were measured. Apart from changes in morphology of plants, alterations in GS and NAD(P)H-GDH activity were reported. GS activity in roots of stressed plants grew slightly and in shoots it decreased by approx. 30% as compared to control plants. In shoots of both control and stressed plants two GS isoforms were detected: cytoplasmic (GS1) and chloroplastic (GS2). A drop in total GS activity in shoots of NaCl-treated plants was due to the drop in activity of GS2 isoform. The activity of GS1 grew slightly under saline stress. After staining of gels for GDH activity, no changes in isoforms were noted as compared with the control plants.

10

Axenic in vitro culture and molecular characterization of Giardia duodenalis from red deer (Cervus elaphus) and Thomson’s gazelle (Gazella thomsonii)

63%

Solarczyk P., Majewska A. C., Slodkowicz-Kowalska A.

Acta Parasitologica

|

2014

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tom 59

|

nr 4

Giardia duodenalis is an ubiquitous flagellate that infects humans and many species of animals. This species exhibits great biotypic and genetic diversity. In the present study, we established short- and long-term in vitro cultures of G. duodenalis trophozoites originating from red deer and Thomson’s gazelle (artiodactyls) and genetically characterised the isolates by their glutamate dehydrogenase and triose phosphate isomerase gene sequences. The G. duodenalis isolates from red deer and the gazelle represented assemblages A (AIII sub-assemblage) and B. In conclusion, G. duodenalis assemblages and sub-assemblages can be associated with differences in growth rate in vitro cultures.

11

Histochemical and biochemical studies on the glutamate transmitter metabolism

63%

Wolf G.

Folia Histochemica et Cytobiologica

|

1984

|

tom 22

|

nr 3-4

12

Importance of glutamate dehydrogenase stimulation for glucose and glutamine synthesis in rabbit renal tubules incubated with various amino acids

63%

Winiarska K., Bozko P., Lietz T., Bryla J.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 3

The effect of 2-aminobicyclo[2.2.1]heptan-2-carboxylic acid (BCH), an L-leucine nonmetabolizable analogue and an allosteric activator of glutamate dehydrogenase, on glucose and glutamine synthesis was studied in rabbit renal tubules incubated with alanine, aspartate or proline in the presence of glycerol and octanoate, i.e. under conditions of efficient glucose formation. With alanine+glycerol+octanoate the addition of BCH resulted in a stimulation of alanine and glycerol consumption, accompanied by an increased glucose, lactate and glutamine synthesis. In contrast, when alanine was substituted by either aspartate or proline, BCH altered neither glucose formation nor glutamine and glutamate synthesis, while an accelerated glycerol utilization was accompanied by a small increase in lactate production. In view of the BCH-induced changes in intracellular metabolite levels the acceleration of gluconeogenesis by BCH in the presence of alanine+glycerol+octanoate is probably due to (i) increased uptake of alanine via alanine aminotransferase, (ii) stimulation of phosphoenolpyruvate carboxykinase, a key-enzyme of gluconeogenesis, (iii) rise of glucose-6-phosphatase activity, as well as (iv)activation of the malate-aspartate shuttle resulting in an augmented glycerol utilization for lactate and glucose synthesis.

13

The involvement of glutamate dehydrogenase in the adaptation of mitochondria to oxidize glutamate in sucrose starved pea embryos

63%

Morkunas I., Lehmann T., Ratajczak W., Ratajczak L., Tomaszewska B.

Acta Physiologiae Plantarum

|

2000

|

tom 22

|

nr 4

14

Metabolism of amino acids in germinating yellow lupine seeds. I. The decomposition of 14 C-aspartate and 14 C-glutamate during the imbibition

63%

Ratajczak W., Lehmann T., Polcyn W., Ratajczak L.

Acta Physiologiae Plantarum

|

1996

|

tom 18

|

nr 1

15

Glutamine synthetase and glutamate dehydrogenase in cadmium-stressed triticale seedlings

63%

Kwinta J., Kozlik D.

Acta Physiologiae Plantarum

|

2006

|

tom 28

|

nr 4

The studies were performed on young triticale seedlings grown on a mineral medium containing 5 mM NO3- as the nitrogen source, with the addition of 0.5 mM CdCl2. It was determined that cadmium ions accumulated mainly in the plant roots. Decreases in nitrate concentrations both in the roots and shoots of seedlings, as well as decreases in soluble protein contents with simultaneous increases in endopeptidase activity were also observed. Both in roots and shoots significant decreases in glutamic acid were noted. Toxic cadmium ion accumulation in seedlings significantly modified activity of primary nitrogen assimilating enzymes, i.e. glutamine synthetase (GS, EC 6.3.1.2) and glutamate dehydrogenase (GDH, EC 1.4.1.2). There was a significant decrease in GS activity both in roots and in shoots of the stressed plants, in compari son to plants grown without cadmium. In shoots of the control plants and plants subjected to stress two GS isoforms were discovered: cytoplasmatic (GSi) and chloroplastic (GS2). Substantial decreases in total glutamine synthetase activity in green parts of seedlings, occurring under stress conditions, result from dramatic decrease in GS2 activity (by 60 % in relation to the control plants); despite simultaneous increases in the cytoplasmatic isoform (GS1) activity by approx. 96 %. Cadmium ions accumulating in roots and shoots of seedlings not only increased GDH activity, but also modified its coenzymatic specificity.

16

Anti-peptide specific antibodies for the characterization of different glutamate dehydrogenase subunits in yellow lupine

63%

Lehmann T., Dabert M., Ratajczak L.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

17

The induction of the alteration of glutamate dehydrogenase [GDH] izoenzymes by cycloheximide in sucrose starved lupine embryos

63%

Lehmann T., Deckert J., Ratajczak L., Przybylska M.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

18

Effect of nickel on ammonium assimilating enzymes in wheat shoots

51%

Gajewska E., Bergier K., Wielanek M., Mazur J., Sklodowska M.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2008

|

tom 524

Effects of two Ni concentrations (50 and 100 µM) on growth, Ni accumulation as well as the activities of glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were studied in shoots of wheat seedlings. Exposure to Ni caused rapid accumulation of this metal in the shoots accompanied by a substantial decrease in the length and fresh weight of these organs. Both aminating (NADH-GDH) and deaminating (NAD-GDH) glutamate dehydrogenase activities were significantly influenced by Ni stress, while GS activity did not change in response to Ni application. The activity of NADH-GDH showed an increase at the end of the experiment and 7 days after Ni treatment it was 68% and 76% higher than in the control, at 50 and 100 µM Ni, respectively. NAD-GDH activity after 1 and 4 days of exposure to a higher concentration of Ni was reduced by 24% and 37%, respectively. However, on the 7th day the activity of this enzyme was enhanced by 150% and 72% over the control level, at 50 and 100 µM Ni, respectively. The obtained results suggest that GDH can play an important role in response of wheat seedlings to Ni toxicity.

19

Cytochemical study of dehydrogenase activity in two euglenid species of the genus Parastasia Michajlow,1966

51%

Wita I.

Acta Protozoologica

|

1989

|

tom 28

|

nr 1

20

Electrophoretic evidence of monomorphism in the Wild Service Tree Sorbus torminalis [L.] Crantz population from Bytynskie Brzeki preserve [Western Poland]

51%

Krzakowa M., Bednorz L.

Rocznik Dendrologiczny

|

1999

|

tom 47

Ograniczanie wyników

Glutamate dehydrogenase [GDH] genes in yellow lupine

Molecular genotyping of Giardia duodenalis in humans in the Andimeshk county, Southwestern Iran

Glutamate dehydrogenase and glutamine synthetase activities during the development of triticale grains

Immunochemical comparison of the glutamate dehydrogenase isoenzymes in yellow lupin root nodules

Glutamate dehydrogenase in higher plants

Effect of selected compounds on the activity of glutamate dehydrogenase from triticale roots

The effect of Cu2plus on uptake and assimilation of ammonium by cucumber seedlings

Biochemical indicators of environmental contamination with heavy metals

Effects of salinity stress on the activity of glutamine synthetase and glutamate dehydrogenase in triticale seedlings

Axenic in vitro culture and molecular characterization of Giardia duodenalis from red deer (Cervus elaphus) and Thomson’s gazelle (Gazella thomsonii)

Histochemical and biochemical studies on the glutamate transmitter metabolism

Importance of glutamate dehydrogenase stimulation for glucose and glutamine synthesis in rabbit renal tubules incubated with various amino acids

The involvement of glutamate dehydrogenase in the adaptation of mitochondria to oxidize glutamate in sucrose starved pea embryos

Metabolism of amino acids in germinating yellow lupine seeds. I. The decomposition of 14 C-aspartate and 14 C-glutamate during the imbibition

Glutamine synthetase and glutamate dehydrogenase in cadmium-stressed triticale seedlings

Anti-peptide specific antibodies for the characterization of different glutamate dehydrogenase subunits in yellow lupine

The induction of the alteration of glutamate dehydrogenase [GDH] izoenzymes by cycloheximide in sucrose starved lupine embryos

Effect of nickel on ammonium assimilating enzymes in wheat shoots

Cytochemical study of dehydrogenase activity in two euglenid species of the genus Parastasia Michajlow,1966

Electrophoretic evidence of monomorphism in the Wild Service Tree Sorbus torminalis [L.] Crantz population from Bytynskie Brzeki preserve [Western Poland]