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The qualitative (TLC) and quantitative (colorimetry and HPLC) composition of saponins (ginsenosides) in Panax quinquefolium originating from the field cultivation in Poland was determined. The saponin content in roots of P. quinquefolium increased with the age of the plant. The largest content of ginsenosides (33-67 mg in 1 g of dry mass) was determined in roots. Especially rich in ginsenosides was a fraction of the finest tertiary roots. Also leaves contained quite high levels of these compounds (over 30 mg/g). Seeds and stems had the lowest content of studied metabolites - about 2 and about 5 mg/g, respectively, of a sum of the six saponins determined. In almost all the raw materials investigated the Re ginsenoside dominated. However, two ginsenosides, namely Re and Rb,, dominated in roots.
In a field experiment conducted in 1998-2002 on sandy-loam soil the effect of applying chosen growth regulators in an American ginseng culture was examined. In the second year of vegetation (June 2000) plants were sprayed with following substances (each in a dose of 100 and 200 mg/1): kinetin, daminozide, mixture (1:1) of gibberellic acid (GAj) with potassium salt of a-naphtyl acetic acid - KNAA and IPO-1 (benzimidazole derivative) - a new preparation obtained from Institute of Organic Industry in Warsaw. Amongst the applied growth regulators positive effect on growth of plants and yield of roots, as well as on the content of active compounds had IPO-1, daminozide (in a dose of 100-200 mg/1) and, to a certain extent, kinetin (200 mg/1), while the mixture of GAj and KNAA affected the above-mentioned features negatively.
We carried out histochemical studies to find the localization of ginsenosides in roots of Panax quinquefolium cultivated in Poland. We performed an anatomical study on the structure and localization of secretory canals on the cross section of 4-year-old American ginseng roots. We observed the occurrence of large secretory canals, mainly in the middle part of the secondary cortex and less in the phloem layer. In our studies, moreover, we demonstrated the production of secretory canals within the periderm layer. After the anatomical study, the 4-year-old ginseng root was divided into periderm, cortex and xylem, and the ginsenosides were extracted from each part of the root. The TLC separation of ginsenosides was performed on silica gel Si60 glass plates with chloroform-methanol-ethyl acetate-water-hexane, 20+22+60+8+4 (v/v) as mobile phase. Quantitative analysis of ginsenosides was performed by using the TLC-densitometric method. Concerning the distribution of ginsenosides in the different anatomical parts of the root of Panax quinquefolium, they were contained in the periderm layer at the highest level.
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