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Przydatnosc metod analizy genomu w diagnostyce giardiozy i kryptosporydiozy

100%

Kasprzak W., Majewska A. C.

Wiadomości Parazytologiczne

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1995

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tom 41

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nr 1

3-11

The article comprises a critical review on practical applications of molecular technology in parasitological diagnostics in a broad sense, also as a diagnosis of species and a method of epidemiological analysis. Techniques of genome analysis at different levels, as specific nucleic acid probes, DNA restriction profiles (RFLP), hybridization techniques, pulse-field gel electrophoresis, in vitro nucleic acid amplification, and DNA fingerprint technique used in studies on Giardia and Cryptosporidium were discussed. The essential reservation as far as this technology is concerned refers to its usefulness in parasitological diagnostics; there is no sense in working out methods for recognizing parasites which could otherwise be identified by well trained parasitologists and simple microscopic methods. The improved diagnosis of parasites resulting from the application of molecular technology significantly contributed to the armentarium of parasitologists. Application of recent molecular technology in diagnosis of giardiosis and cryptosporidiosis may basically support clinical diagnosis which provides possibilities of early and selective treatment and makes possible epidemiological studies. These assays will permit not only a rapid diagnosis and exact differentiation but will also enable a better recognition of Giardia and Cryptosporidium genome organization. However, in spite of the wide availability of this new techniques they have not been fully applied - as yet – in diagnosis and in epidemiological studies on these parasites. The authors share the opinion of BUSCH (1991) on the need of proper recognition of high-quality and rigorous work in employing new molecular assays, because their wide availability and high sensitivity could cause "false-positive" results by contamination with amplified DNA sequences. They totally support the conclusion made by THOMPSON and MELONI (1993) that the molecular data must be fairly interpreted by collaborating molecular biologists, parasitologists, and epidemiologists - and clinicians as well - to avoid forming of a confusing picture of genetic diversity of the parasites with no practical application.

2

Konferencja pt.Aspekty kliniczne wybranych chorob pasozytniczych u ludzi [toksoplazmoza, toksokaroza, giardioza]

84%

Blaszczy B.

Wiadomości Parazytologiczne

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1997

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tom 43

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nr 1

132-133

Pneumocystosis (Pneumocystis carinii pneumonia) is a parasitic disease which in Lithuania used to be diagnosed only with the use of pathohistological methods. The aim of our study was to determine prevalence of P. carinii antibodies among children and women, using immunochemical method (ELISA) and to find out which groups of patients have higher antibody titres. We also addressed the question of infection with P. carinii among rodents. After investigating 9 species of rodents it was found that mostly infected is brown rat (Rattus norvegicus) - 42.9% of them were found infected. The least infected was bank vole (Clethrionomys glareous)- 5.9. %. The brown rat lives on hatch of domestic birds, rabbits or drinks eggs, while rodents in nature live on insects, molluscs, amphibians etc. European water voles (Arvicola terrestris) and field voles (Microtus arvalis) are vegetable feeders. Our data shows that those species of rodents were not infected with pneumocysts.

3

Choroby odzwierzece w codziennej praktyce lekarza

84%

Wiercinska-Drapalo A., Flisiak R., Prokopowicz D.

Medycyna Weterynaryjna

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1996

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tom 52

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nr 05

286-288

4

Wartosc diagnostyczna testow wykrywajacych swoiste przeciwciala Giardia

84%

Kurczewska M., Paul M., Pawlowski Z., Skoryna B.

Wiadomości Parazytologiczne

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1994

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tom 40

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nr 1

11-14

Two commercial faecal antigen tests, based on Giardia specific antigens, were compared with traditional microscopical examination of faeces in 40 clinical patients, including 14 patients with a confirmed Giardia infection as well as in a survey among 96 children in four day care centers. The results obtained with these faecal antigen tests were not much better than by using traditional microscopy by a well trained parasitologist. Specific Giardia antigen (GSA 65) has been still detected in faeces up to day 4th after treatment of giardiosis, when microscopical examination of faeces was already for a few days negative. The Giardia antigen detection test was not very useful in examining the unconcentrated duodenal content.

5

Epidemiologiczne aspekty giardiozy (lambliozy) u ludzi

84%

Glowniak C.

Wszechświat

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1989

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tom 90

|

nr 12

6

Rozpoznawanie powiklanej giardiozy u psow [na podstawie przypadkow wlasnych]

84%

Lukaszewska J., Pacon J.

Magazyn Weterynaryjny

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2009

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tom 18

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nr 04

284-287

7

Epidemiologiczne badania nad giardioza u mieszkancow Szczecina

84%

Ramisz A., Krolikowska A., Balicka-Ramisz A.

Wiadomości Parazytologiczne

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1999

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tom 45

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nr 1

69-74

The aim of the studies was to establish the prevalence of Giardia intestinalis in Szczecin population. The studies were carried out in 1991-1996. In this period 46 584 persons - 22 371 adults and 24 213 children were examined. Faecal samples were examined with two methods - coprological and immunoenzymatic (ELISA) test. A total of 356 persons (0.78%) were found to be infected with G. intestinalis. No statistically significant differences between the infection rate of adults and children were observed. The adults were infected in 0.73% and the children in 0.80%. The analysis of the annual dynamics of G. intestinalis infection shows twice as large infection rate in September- October (1.18 %) as in the remaining months (0.66 %).

8

Prewalencja inwazji Giardia duodenalis u psów i kotów z terenu Wrocławia i Dolnego Śląska

84%

Polozowski A., Piekarska J., Pacon J., Zawadzki W., Bednarz-Nabzdyk R., Stochnij P., Cislo-Pakuluk A.

Magazyn Weterynaryjny

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2011

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tom 20

|

nr 02

9

Zoonozy - choroby odzwierzece [III]

84%

Przytula J.

Poradnik Gospodarski

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2001

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nr 06

26-27

10

Rola zwierzat jako rezerwuaru giardiozy u ludzi - zoonotyczny potencjal Giardia intestinalis

84%

Zygner W., Wedrychowicz H.

Postępy Mikrobiologii

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2008

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tom 47

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nr 3

287-291

11

Patofizjologiczne i immunologiczne aspekty giardiozy

84%

Jaros D.

Życie Weterynaryjne

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2007

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tom 82

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nr 06

483-486

12

Giardioza psow oraz jej zwalczanie przy uzyciu fenbendazolu

84%

Ramisz A., Prost M., Balicka-Ramisz A.

Życie Weterynaryjne

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2000

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tom 75

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nr 09

481-482

13

Uklad Giardia - zywiciel: zmiennosc obrazu inwazji

84%

Majewska A. C.

Wiadomości Parazytologiczne

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1994

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tom 40

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nr 1

3-9

In man, as well as in many species of vertebrates there exist several populations of Giardia duodenalis group which, though morphologically indistinguishable, show different level of heterogenicity in several characteristics, a. o. in surface antigens, isoenzyme make-up, RFLP, invasiveness for different hosts or pattern of experimental infection. Also the clinical observations in man distinctly suggested that G. intestinalis comprises several different populations. In the course of giardiosis apparent variability in clinical manifestations can be observed. In many patients the infection is symptomless and resolves spontaneously and in some others – variable intensity of symptoms is observed. Most likely both the parasit's characteristics and the host's feature will determine the clinical character of infection. It is well known that in some cases it is the host that responsible for the symptoms of the infection. Thus, for instance, clinical giardiosis links with immunodeficiency, malnutrition or young age. There are also evidences that some Giardia isolates have enhanced potency to provoke the disease of the host. It is considered that the variable pattern of the infection may be related to three different factors in host-parasite relationship: extra- and intrapopulation variability of Giardia isolates, the microenvironmental factors of the host's intestine, and the variable immune response of the host. The author describes data on the two first factors.

14

Wybrane problemy z 'zycia wewnetrznego' psow - przeglad najwazniejszych inwazji pasozytow jelitowych

67%

Klockiewicz M.

Magazyn Weterynaryjny

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2004

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tom 13

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nr 09

55-58

15

Metabolizm serotoniny u dzieci zarazonych Giardia intestinalis

67%

Lucinska A., Prusek W., Galary E., Podwysocka M., Modrzycka T.

Wiadomości Parazytologiczne

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2000

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tom 46

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nr 1

149-155

According to the data from the last 4 years, 3.8% of all diagnosed parasitoses are giardiases. During 5 years Giardia intestinalis was found in 24 chiidren hospitalized in the General Hospital of the Wrocław Voivodship: in 14 girls and 10 boys, 2-12 years old. The parasite was identified coproscopically and on the basis of clinical symptoms. Before the medication, the blood was taken from each child to determine the serotonin level and the (MAO) monoaminooxydaze, enzyme metabolizing serotonin acivity in the blood serum. The results were compared to the values obtained in the control group of 10 healthy children, 3-12 years old. III children were divided into two groups: first consisted of 12 children, 2-5 years old, second - of 12 children, 6-12 years old. In the first group 210% increase of the serotonin level in the blood and slight 32,4% increase of the MAO activity were observed in comparison with the control group. In the second group the serotonin level in the blood increased 221 % in comparison with the control, while the MAO actmty increased 45.1 %. In older children marced clinical symptoms could be observed, mainly malfunction of the alimentary tract, microelements shortage and anemia. The highest level of serotonin and MAO activity was found in the child with bile duet irritation. The reason for the increased level of serotonin and MAO activity can be pathogenic action of parasite's trophozoites and cysts on the jejunum mucosa and mobilization, proliferation and degranulation of tissue mastocytes. Serotonin, released from mastocytes, can be the cause of enhanced peristalsis and mucosa congestion.

16

Pasożytnicze zoonozy jako zagrożenie dla lekarza weterynarii w praktyce małych zwierząt

67%

Pacon J.

Weterynaria w Praktyce

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2011

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tom 08

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nr 05

17

Giardioza u psów i kotów: patogeneza - powikłania - frustracje. Cz. I

67%

Zygner W.

Weterynaria w Praktyce

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2019

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tom 16

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nr 10

18

Przegląd chorób powakacyjnych u psów i kotów

67%

Adaszek L., Lyp P., Szczepaniak K., Zietek J., Surma-Kurusiewicz K., Winiarczyk S.

Weterynaria w Praktyce

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2016

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tom 13

|

nr 06

19

Giardioza kością niezgody? Nowe perspektywy leczenia

67%

Klockiewicz M.

Magazyn Weterynaryjny

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2012

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tom 21

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nr 06

20

Pasożyty jelitowe psów i kotów - złoty standard czy modne trendy diagnostyczne?

67%

Janczak D.

Weterynaria w Praktyce

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2019

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tom 16

|

nr Dodatek Żywieniowy. Grudzień 2019

Ograniczanie wyników

Przydatnosc metod analizy genomu w diagnostyce giardiozy i kryptosporydiozy

Konferencja pt.Aspekty kliniczne wybranych chorob pasozytniczych u ludzi [toksoplazmoza, toksokaroza, giardioza]

Choroby odzwierzece w codziennej praktyce lekarza

Wartosc diagnostyczna testow wykrywajacych swoiste przeciwciala Giardia

Epidemiologiczne aspekty giardiozy (lambliozy) u ludzi

Rozpoznawanie powiklanej giardiozy u psow [na podstawie przypadkow wlasnych]

Epidemiologiczne badania nad giardioza u mieszkancow Szczecina

Prewalencja inwazji Giardia duodenalis u psów i kotów z terenu Wrocławia i Dolnego Śląska

Zoonozy - choroby odzwierzece [III]

Rola zwierzat jako rezerwuaru giardiozy u ludzi - zoonotyczny potencjal Giardia intestinalis

Patofizjologiczne i immunologiczne aspekty giardiozy

Giardioza psow oraz jej zwalczanie przy uzyciu fenbendazolu

Uklad Giardia - zywiciel: zmiennosc obrazu inwazji

Wybrane problemy z 'zycia wewnetrznego' psow - przeglad najwazniejszych inwazji pasozytow jelitowych

Metabolizm serotoniny u dzieci zarazonych Giardia intestinalis

Pasożytnicze zoonozy jako zagrożenie dla lekarza weterynarii w praktyce małych zwierząt

Giardioza u psów i kotów: patogeneza - powikłania - frustracje. Cz. I

Przegląd chorób powakacyjnych u psów i kotów

Giardioza kością niezgody? Nowe perspektywy leczenia

Pasożyty jelitowe psów i kotów - złoty standard czy modne trendy diagnostyczne?