Wyniki wyszukiwania - AGRO

1

Labelling of Anagallis arvensis cells with fluorescent marker gene GFP by means of biolistic transformation

100%

Lukaszewicz M., Kwiatkowska D.

Folia Morphologica

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1998

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tom 57

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nr 1

2

Genetic diversity in the raccoon dog [Nyctereutes procyonoides Gray, 1834] an analysis based on molecular markers

100%

Slaska B., Jezewska G.

Folia Universitatis Agriculturae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica

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2007

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tom 03

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nr 3

3

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Genetic aspects of mastitis resistance in cattle

100%

Walawski K.

Journal of Applied Genetics

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1999

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tom 40

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nr 2

4

Dependence of aerobic performance of athletes on polymorphism of genes

86%

Drozdovska S. B., Lysenko O. M., Dosenko V. E., IIyin V. N.

Central European Journal of Sport Sciences and Medicine

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2015

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tom 09

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nr 1

The adaptation of an athlete to systematic physical exercise has been shown to be determined by a combination of great many genes. The aim of our study was to investigate the dependence of the aerobic capacity parameters in sport on the set of gene polymorphisms. Cardio-respiratory system (CRS) adaptation reactions to exercise of 72 endurance athletes were assessed using the gas analysis. The analysis of the obtained results has shown both single and combined effect of the gene polymorphisms on the aerobic capacity. The impact of 6 polymorphisms on the aerobic performance level was analyzed: Т–786→С polymorphism of the promoter of еNOS gene as well as АСЕ I/D polymorphism, Рго/Ala polymorphism of PPARG gene, G/C polymorphism of PPARA gene, Pro582Ser polymorphism of HIF1α gene, and Ala203Pro polymorphism of PPARGC1B. It was found that a single impact on the HRmax providing АСЕ I/D polymorphism. Individual influence of АСЕ gene accounts for 2% of this index dissipation. Results showed that there is a dependence between the amount the maximum volume of consumed oxygen (VO2max) from the set of gene polymorphisms. Cumulative impact of these polymorphisms in the combination with the individual parameters (gender; qualification; kind of sport) stipulates 71% of dispersion of VO2max value.

5

Genetic characterization and analysis of the population of selected Sarawak rice cultivars using simple sequence repeat markers

86%

Razak S. A., Saidon S. A., Yusof M. F. M., Ismail S. N.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2018

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tom 99

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nr 3

6

Evaluation of changes in the genetic structure of Hucul horses based on the analysis of I type of genetic markers

86%

Nogaj A., Nogaj J., Kozubska-Sobocinska A., Rychlik T.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

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2013

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tom 31

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nr 2

7

DNA polymorphism of the alphaA-globin gene in domestic pigeon

86%

Dybus A., Chang M.-H., Cheng Y.-H., Szatkowska I.

Animal Science Papers and Reports

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2008

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tom 26

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nr 3

The study aimed at identifying the polymorphism of domestic pigeon αA-globin gene which can be a potential homing marker in selection of racing pigeons. A total of 329 domestic pigeons were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP)method. The PCR products were digested with 17 restriction endonucleases. One RFLP – detected with HphI – was found in intron 1 and represented a C/T mutation. The second RFLP – detected with BseLI – was a point mutation in the 3’UTR region of the gene (C/T mutation). The polymorphism in the 3’UTR region of the pigeon αA-globin gene can potentially affect the stability of mRNA and modify the gene expression. The mechanisms of haemoglobin function reflecting variants of the αA-globin gene remain unknown.

8

Insertion of a reamplification round into the ISSR-PCR protocol gives new flax fingerprinting patterns

86%

Wiesner I., Wiesnerova D.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 3

We expanded the basic ISSR-PCR protocol by an additional PCR reamplification round in order to detect whether increased PCR productivity would give new bands in ISSR patterns. We found that the reamplification step had a prominent impact on the quality of the inter-simple-sequence repeat (ISSR) PCR patterns of flax, depending on the particular primer used for PCR amplification. We could clearly distinguish between two types of reamplification effect. Most ISSR primers (16 out of 21) gave no reamplification effect as usual, but five primers (23.8%) provided a new ISSR fingerprinting pattern after the 2nd reamplification round, leaving the previous 1st round pattern completely blank. Therefore, we recommend the expansion of a basic ISSR-PCR protocol for another reamplification round in order to mine out full the fingerprinting potential from ISSR-PCR method.

9

In vivo labelling of Anagallis arvensis L. cells with green fluorescent protein

86%

Lukaszewicz M., Kwiatkowska D.

Acta Societatis Botanicorum Poloniae

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2000

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tom 69

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nr 2

10

Black Pied Prestice - a gene reserve of pigs in the Czech Republic

86%

Horak P., Mikova G., Vrtkova I., Dvorak J.

Annals of Animal Science. Supplement

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2001

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nr 1

11

Selected genetic markers: their effect on milk production traits

86%

Cernekova V., Dudkova G., Kott T., Stankova Z., Soldat J., Markova M.

Animal Science Papers and Reports. Supplement

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2004

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tom 22

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nr 2

12

AFLP marker polymorphism in cucumber [Cucumis sativus L.] near isogenic lines differing in sex expression

86%

Witkowicz J., Urbanczyk-Wochniak E., Przybecki Z.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2

The AFLP technique was used to evaluate the level of polymorphism between two pairs of isogenic cucumber (Cucumis sativus L.) lines (NIL) differing in flower sex expression. The BSA techniques were also applied to find molecular markers linked to sex determination genes (dominant alleles) in those cucumber lines. Sex determination in cucumber is controlled by three main bands: F, M and Gy. The interaction of these bands is responsible for the formation of the various flower phenotypes with respect to sex in the analyzed lines: a female line 2gg with a ff/MM/gygy genotype, isogenic to a monoecious line B10 (genotype ff/MM/GyGy), and a female line Gy3 with a FF/MM/GyGy genotype, isogenic to a hermaphroditic line HGy3 (genotype FF/mm/GyGy). Using 56 combinations of AFLP primers, used for the analysis of lines 2gg and B10, gave 3794 bands, of which 155 (4.1%) were polymorphic. Ten bands distinguished gynoecious and monoecious bulks appearing at the same time in the appropriate parent; they are believed to be linked to the Gy locus. The isogenic lines Gy3 and HGy3 showed a higher level of polymorphism (14.2%). In this case, 55 combinations of primers gave 2996 reaction products, of which 430 showed variation. Twenty bands occurred in one bulk and in one parent, so they are probably associated with the M locus. Using the AFLP technique, the isogenicity of the lines was evaluated. The level of polymorphism (per pair of primer) between lines 2gg and B10 is 0.072% and is four times lower than that between the Gy3 and HGy3 lines (0.27%). The differences in the isogenicity of the lines can result from the degree of their relatedness, which may reflect the way they were derived.

13

Seed sexing revealed female bias in two Rumex species

72%

Kwolek B., Joachimiak A.

Acta Societatis Botanicorum Poloniae

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2011

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tom 80

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nr 2

Sex-ratio bias in seeds of dioecious Rumex species with sex chromosomes is an interesting and still unsettled issue. To resolve gender among seeds of R. acetosa and R. thyrsiflorus (two species with an XX/XY1Y2 sex chromosome system), this work applied a PCR-based method involving DNA markers located on Y chromosomes. Both species showed female-biased primary sex ratios, with female bias greater in R. acetosa than in R. thyrsiflorus. The observed predominance of female seeds is consistent with the view that the female biased sex ratios in Rumex are conditioned not only postzygotically but also prezygotically

14

The evidence of a null allele in the esterase D in the Polish population

72%

Turowska B., Klys M., Turowski G., Nowicka L.

Archivum Immunologiae et Therapiae Experimentalis

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1989

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tom 37

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nr 3-4

15

Genetic basis of mastitis resistance in dairy cattle - a review

72%

Sender G., Korwin-Kossakowska A., Pawlik A., Hameed K. G. A., Oprzadek J.

Annals of Animal Science

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2013

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tom 13

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nr 4

16

Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates

72%

Derda M., Wojtkowiak-Giera A., Hadas E.

Acta Parasitologica

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2014

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tom 59

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nr 3

Acanthamoeba are widespread free-living amoebae which may cause granulomatous amoebic encephalitis (GAE), keratitis, skin ulcerations and disseminated tissue infection. An important diagnostic and prognostic factor for the treatment of infection is a quick and correct diagnosis of amoebae strains. The aim of our study was to develop a rapid method for detection and identification of pathogenic Acanthamoeba spp. strains from diagnostic material collected from water. In this study we analysed five amplification-based genetic markers (Aca 16S, Ac6/210, GP, JDP, Nelson) used for identification of pathogenic Acanthamoeba spp. strains isolated in water sources in Poland, Iceland and Sweden. Our results demonstrated the presence of pathogenic Acanthamoeba strains in tap water. PCR assay appeared to be a more rapid and sensitive method to detect the presence of amoebae than the limited conventional techniques. Based on our observations, we can confirm that the use of four out of five genetic markers (Aca 16S, Ac 6/210, JDP, GP, Nelson) may be helpful in identification of Acanthamoeba spp. strains, but only one Aca 16S primer pair is a highly specific marker that distinguishes between pathogenic strains of Acanthamoeba and other free-living amoeba families.

17

Artificial gynogenesis in common bream (Abramis brama (L.)) induced by cold-temperature shock

72%

Kucharczyk D., Luczynski M. J., Szczerbowski A., Woznicki P., Luczynski M., Targonska-Dietrich K., Kujawa R. J., Mamcarz A.

Archives of Polish Fisheries

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2004

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tom 12

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nr 2

Bream, Abramis brama (L.), eggs fertilized with genetically inactivated sperm (UV irradiation dose of 1920 J m-2) were exposed to thermal cold shock to produce meiotic gynogenotes. The shock was applied at one-minute intervals from 1 to 10 min after egg insemination. The temperature of the shock was 2.0 ± 0.1°C, and its duration was 45 min. The water temperature prior to the shock was 20.0°C. Eggs fertilized with genetically inactivated sperm (putative haploids) exhibited retarded and abnormal development. The yield of gynogenesis was relatively low, except for the group to which the shock was applied 1 min after fertilization (about 30% in comparison with the controls). Ninety fish from the control and gynogenetic groups were reared for ten months. The survival of the gynogenetic bream was twofold lower than that of the controls. The gynogenotes were highly variable in size and exhibited some morphological abnormalities. The sex ratios in the control groups were close to 1:1, whereas all the gynogenotes were female.

18

The estimation of genetic variation within and between Polish provenances of Norway spruce (Picea abies (L.) Karst.) on the basis of RAPD polymorphism

72%

Kraj W.

Electronic Journal of Polish Agricultural Universities. Series: Forestry

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2002

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tom 05

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nr 2

19

Markery genetyczne u swin, ich znaczenie i wykorzystanie w praktyce hodowlanej

72%

Parys-Proszek A.

Biuletyn Informacyjny. Instytut Zootechniki

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1998

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tom 36

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nr 4

15-21

20

Juniperus excelsa s. str. in Crimea - differentiation and history inferred from genetic and morphological markers

72%

Mazur M., Jadwiszczak K. A., Bona A., Krasylenko Y., Kukushkin O., Marcysiak K.

Folia Forestalia Polonica. Series A . Forestry

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2021

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tom 63

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nr 4

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