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1

Single-strand conformation polymorphism within the porcine growth hormone gene promoter

100%
Kaminski S., Wachek M.
Animal Science Papers and Reports
|
2002
|
tom 20
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nr 1
2

Mechanisms of transcription regulation

86%
Poluha D.
Applied Biology Communications. Lublin Scientific Center
|
1991
|
tom 01
|
nr 6
3
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Content available

WRKY transcription factors in response to ROS

86%
Vaahtera L., Koskela M., Jolma A., Nurkkala H., Varjosalo M., Brosche M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
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tom 94
|
nr 2
4

Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

86%
Pluskota W. E., Michalczyk D. J., Gorecki R. J.
Acta Physiologiae Plantarum
|
2005
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tom 27
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nr 2
The gene fusion system was used to study UV light-control of PS PAL1 and PS PAL2 genes encoding phenylalanine ammonia-lyase of pea. The induction of pea PAL promoters was analysed in transgenic tobacco plants. Binary plasmids (derivatives of pBI101.2 vector) containing 5’ regulatory fragments of PS pAl1 and PS PAL2 linked to reporter genes (GUS, LUC) were constructed. The analyses were performed with the use of single constructs (containing one variant of PS PAL promoter and one reporter gene) and dual constructs (containing both PS PAL1 and PS PAL2 promoters connected with different reporter genes). The use of dual constructs enabled the evaluation of both PS PAL promoters activity in the same plant. The analyses of in vitro grown plants have shown that both PAL promoters are strongly induced in leaves subjected to UV radiation. In some cases, the UV-stimulated expression exceeded the exposed areas. This phenomenon was observed more often in the leaves of plants containing the PS PAL1:: GUS than PS PAL2::GUS construct. Removal ofboxes 2, 4, 5 from PS PAL1 promoter and deletion of its 5’end region (-339 to -1394) decreases the level of gene expression but does not eliminate its responsiveness to UV.
5

Functional analysis of spermatocyte-specific hst70 gene promoter in transgenic mice

86%
Widlak W., Markkula M., Krawczyk Z., Huhtaniemi I.
Acta Biochimica Polonica
|
1994
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tom 41
|
nr 2
6

Nuclear localization and binding affinity of STAT5b for the alpha2-macroglobulin gene promoter during rat liver development and the acute-phase response

72%
Mihailovic M., Dinic S., Bogojevic D., Ivanovic-Matic S., Uskokovic A., Arambasic J., Grigorov I., Grdovic N., Vidakovic M., Martinovic V., Petrovic M., Poznanovic G.
Acta Biochimica Polonica
|
2007
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tom 54
|
nr 2
Expression of the rat α2-macroglobulin (MG) gene undergoes dynamic changes throughout an individual's life and during the acute-phase (AP) response. Details of the participation of the STAT family of transcription factors in its control remain incompletely understood. Here we examined the involvement of STAT5b in MG gene expression during development and the AP response. Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal development, whereas in the adult it decreased. Stimulation of MG expression during the AP response was accompanied by a decrease in STAT5b. Examination of STAT5b localization revealed that the relative concentrations of STAT5b were higher in the nuclear matrix than in the nuclear extract. Affinity chromatography with the extended promoter region of the MG gene (-825/+12), followed by immuno-blot analysis, revealed dynamic changes in STAT5b binding. The highest concentration of the promoter-binding form of STAT5b was observed in the fetus. As postnatal development progressed, the level of promoter-bound STAT5b decreased and in the adult liver it was the lowest. Stimulation of MG gene expression during the AP response in both the fetus and adult was accompanied by significantly decreased STAT5b binding to the MG promoter. The AP response was accompanied by lower levels of STAT5b serine and tyrosine phosphorylation in both fetus and adult. In the nuclear matrix derived from adult tissues, tyrosine phosphorylated species were completely absent. We conclude that developmental-stage differences in the mechanisms that determine STAT5b nuclear localization contribute to its activity in vivo.
7

Genes expressed in cattle mammary gland - computational analysis of 5' -upstream sequences in search for factors conferring a tissue- and stage-specific transcription

72%
Malewski T., Zwierzchowski L.
Animal Science Papers and Reports
|
2002
|
tom 20
|
nr 1
8

Concomitant hypermethylation of multiple genes in non-small cell lung cancer (NSCLC)

58%
Ji M., Guan H., Shi B., Hou P.
Folia Histochemica et Cytobiologica
|
2011
|
tom 49
|
nr 1
9

Overexpression of suadea salsa S-adenosylmethionine synthetase gene promotes salt tolerance in transgenic tobacco

58%
Qi Y.-C., Wang F.-F., Zhang H., Liu W.-Q.
Acta Physiologiae Plantarum
|
2010
|
tom 32
|
nr 2
The suadea salsa full-length S-adenosylmethionine synthetase (SsSAMS2) was introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium tumefaciens-mediated transformation. The gene transformation and expression in tobacco were confirmed by PCR, RT-PCR and Northern blotting analysis. Several transgenic lines (ST lines) overexpressing SsSAMS2 gene under the control of cauliflower mosaic virus 35S promoter showed more seeds number and weight, and accumulated higher free total polyamines (PAs) than wild-type plants (WT lines) and transformants with blank vector (BT lines). Salt stress-induced damage was attenuated in these transgenic plants, in the symptom of maintaining higher photosynthetic rate and biomass. These results that the transgenic plants overexpressing suadea salsa SAMS2 are more tolerant to salt stress than wild-type plants suggest that PAs may play an important role in contributing salt tolerance to plants.
10

Plasmodium falciparum Pfs25 gene promoter has no polymorphism in natural isolates of Eastern India

58%
Raj D. K., Mishra S., Das B. R., Dash A. P.
Acta Protozoologica
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2005
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tom 44
|
nr 4
11

Regulation of cell specific expression of calcyclin [S100A6] in nerve cells and other tissues

58%
Lesniak W., Swart G. W. M., Bloemers H. P. J., Kuznicki J.
Acta Neurobiologiae Experimentalis
|
2000
|
tom 60
|
nr 4
Many of the small, acidic, calcium binding S100 proteins present in the brain specifically map different anatomical regions and cell types and their overexpression is implicated in pathological changes. Similarly to other members of the S100 protein family, calcyclin (S100A6) is expressed in a cell specific manner and is found in subpopulations of neurons and astrocytes in the brain and in epithelial cells and fibroblasts. In this article we review data concerning the cell specific expression of S100 protein genes and present experimental results on the regulation of the calcyclin gene. We have performed promoter deletion studies to locate regions within the calcyclin gene promoter responsible for transcriptional regulation. The results demonstrate that the 3 kb long calcyclin gene promoter lacks a cell specific cis-acting element and drives the expression of the reporter gene also in cells that do not express endogenous calcyclin. The expression is modulated by positive and negative elements acting uniformly in the four different cell lines studied. The first intron of the calcyclin gene was found to have an inhibitory influence on expression regardless of cell type. It was also shown that calcyclin expression can be induced in calcyclin-negative cells by treatment with 5-azacytidine suggesting the involvement of gene methylation in its cell specific expression. The results are discussed in light of the data available on the regulation of other S100 genes.
12
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Content available

Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]

58%
Sadowski J., Rorat T., Cooke R., Delseny M.
Journal of Applied Genetics
|
1997
|
tom 38
|
nr 3
The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
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