Wyniki wyszukiwania

1

Spontaneous mutagenesis in exponentially growing and stationary-phase, umuDC-proficient and -deficient, Escherichia coli dnaQ49

100%

Nowosielska A., Nieminuszczy J., Grzesiuk E.

Acta Biochimica Polonica

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2004

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tom 51

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nr 3

Spontaneous mutations arise not only in exponentially growing bacteria but also in non-dividing or slowly dividing stationary-phase cells. In the latter case mutations are called adaptive or stationary-phase mutations. High spontaneous mutability has been observed in temperature sensitive Escherichia coli dnaQ49 strain deficient in 3 '=>5' proofreading activity assured by the £ subunit of the main replicative polymerase, Pol III. The aim of this study was to evaluate the effects of the dnaQ49 mutation and deletion of the umuDC operon encoding polymerase V (Pol V) on spontaneous mutagenesis in growing and stationary-phase E. coli cells. Using the argE3oc =>Arg+ reversion system in the AB1157 strain, we found that the level of growth-dependent and stationary-phase Arg+ revertants was significantly increased in the dnaQ49 mutant at the non-permissive temperature of 37°C. At this temperature, in contrast to cultures grown at 28°C, SOS functions were dramatically increased. Deletion of the umuDC operon in the dnaQ49 strain led to a 10-fold decrease in the level of Arg+ revertants in cultures grown at 37°C and only to a 2-fold decrease in cultures grown at 28°C. Furthermore, in stationary-phase cultures Pol V influenced spontaneous mutagenesis to a much lesser extent than in growing cultures. Our results indicate that the level of Pol III desintegration, dependent on the temperature of incubation, is more critical for spontaneous mutagenesis in stationary-phase dnaQ49 cells than the presence or absence of Pol V.

2

Altering substrate specificity of catechol 2,3-dioxygenase from Planococcus sp. strain S5 by random mutagenesis

86%

Hupert-Kocurek K., Wojcieszynska D., Guzik U.

Acta Biochimica Polonica

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2014

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tom 61

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nr 4

3

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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

86%

Woloszyn A., Kotlowski R.

Roczniki Państwowego Zakładu Higieny

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2017

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tom 68

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nr 3

Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.

4

Lack of salidroside impact on selected cytochromes encoding genes transcription in the liver of ethanol induced rats

86%

Kujawski R., Szulc M., Tobola M., Graczyk M., Czora-Poczwardowska K., Baraniak J., Kania-Dobrowolska M., Slynko-Krzyzostaniak J., Krajewska-Patan A., Mikolajczak P. L.

Herba Polonica

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2021

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tom 67

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nr 3

5

Cucumber HMA5A and HMA5B are two tonoplast-localized homologs of Arabidopsis thaliana heavy metal ATPase HMA5 involved in cellular heavy metal homeostasis

86%

Migocka M., Papierniak A., Posyniak E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

|

nr 3

6

Thermostable Pyrococcus woesei beta-D-galactosidase - high level expression, purification and biochemical properties

86%

Wanarska M., Kur J., Pladzyk R., Turkiewicz M.

Acta Biochimica Polonica

|

2005

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tom 52

|

nr 4

The gene encoding β-D-galactosidase from Pyrococcus woesei was PCR amplified, cloned, expressed in Escherichia coli under the control of an inducible T7 promoter, purified and characterized. The expression system was developed by the construction of recombinant plasmid, based on the high copy number pUET1 vector, giving four times more efficient expression of P. woesei β-D-galactosidase (20 mg of enzyme from 1 liter of culture) than that obtained from a previously constructed one. The recombinant enzymes were purified in a two-step procedure: double heat-denaturation of E. coli cell proteins and affinity chromatography on p-aminobenzyl 1-thio-β-D-galactopyranoside-agarose. To achieve efficient purification of P. woesei β-D-galactosidase by immobilized metal-ion affinity chromatography (IMAC), a His-tag was placed either at the N- or the C-terminal of the coding sequence. The obtained fusion proteins revealed the same specific activity of approximately 5400 U/mg, which was 10 times lower than the wild-type β-D-galactosidase (51100 U/mg). The activity of P. woesei β-D-galactosidase was enhanced by thiol compounds, Mg2+ ions and D-galactose, and was inhibited by heavy metal ions and D-glucose, while Ca2+ ions had no effect.

7

Cloning and characterization of the gene encoding ribosomal PO phosphoprotein from Neurospora crassa

86%

Krokowski D., Tchorzewski M., Grankowski N.

Acta Biochimica Polonica

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2002

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tom 49

|

nr 1

A gene for ribosomal protein P0 that belongs to the family of ribosomal P proteins was isolated from a Neurospora crassa cDNA library, using polyclonal antibodies against recombinant P0 protein from Saccharomyces cerevisiae. This is the first gene for ribosomal P0 protein to be cloned from filamentous fungi. The derived P0 protein sequence has a strong homology to other eukaryotic P0 proteins; yet, there is a notable alteration in the conservative C-terminal region, placing this protein among the unique sequences from protozoan parasites.

8

Identification and characterization of two XTH related genes involved in cucumber [Cucumis sativus L.] somatic embryogenesis

86%

Malinowski R., Tagashira N., Filipecki M., Gaj P., Malepszy S.

Polish Journal of Natural Sciences. Supplement

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2003

|

tom 01

9

Development of a multiplex PCR [m-PCR] test for rapid identification of genes encoding heat-labile [LTI] and heat-stable [STI and STII] toxins of enterotoxigenic Escherichia coli [ETEC] with internal control of amplification

86%

Weiner M., Osek J.

Polish Journal of Microbiology

|

2004

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tom 53

|

nr 1

A multiplex PCR system was developed for specific identification of genes encoding heat-labile (LTI) and heat-stable (STI and STII) toxins of enterotoxigenic Escherichia coli (ETEC) strains. In addition, primers specific for the E. coli gene coding for 16S rRNA were used as an internal control of the DNA amplification. The specificity of the method was validated by single PCR tests performed with reference to E. coli strains as well as pig-isolated bacteria and 100% correlation was observed. The developed multiplex PCR allowed rapid and specific identification of enterotoxin-positive E. coli and may be used as a sensitive and specific method for a direct determination of ETEC and to differentiate them from other E. coli isolates.

10

Bundle sheath-specific expression of the ascorbate peroxidase 2 affects photosynthesis in whole plant

72%

Gorecka M., Alvarez Fernandez R., Bialasek M., Mulleineaux, Karpinski S.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 2

11

Phylogenetic analysis of selected strains of rabbit haemorrhagic disease virus on the basis of N-terminal fragment of the gene encoding structural protein VP60

72%

Pawlikowska M., Hukowska-Szematowicz B., Deptula W.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

|

nr 2

129-133

The purpose of the study was the phylogenetic analysis of 16 European strains of the rabbit haemorrhagic disease (RHD) virus identified in 1989-2004, on the basis of N-terminal fragment of the gene encoding structural protein VP60. The obtained sequences of the strains were compared to the sequences of 30 strains of the virus received from the GenBank gene database. As a result of phylogenetic analysis of all the strains, they were divided into six genogroups, which were principally formed on the basis of the time of their identification, and the place of isolation of the RHD virus, which confirms the present hypothesis of genogroups creation among this virus. Furthermore, in our studies, a clear and visible difference was obtained between the classic strains in genogroups 1-5, and the strains referred to as antigen variants - genogroup 6.

12

Salivaricin B gene - its localization and RFLP analysis in two potentially probiotic Lactobacillus salivarius strains

72%

Kizerwetter-Swida M., Binek M.

Bulletin of the Veterinary Institute in Puławy

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2010

|

tom 54

|

nr 4

The study has provided data on two Lactobacillus salivarius strains of poultry origin. The strains were investigated for the presence of genes encoding a bacteriocin: salivaricin B, and their localisation in chromosome or plasmid DNA. Specific primers were used to amplify a 224 bp fragment of salivaricin B gene (salB). RFLP analysis of PCR products revealed two DNA fragments of the predicted sizes upon digestion with Ndel and Xapl. Analysis with SspI allowed obtaining variability in two fragments in comparison to computer analysis of both strains, suggesting their divergency. It may be assumed that the amplified DNA fragments of salB gene share great, but not complete similarity to the previously described sequence of salB gene. Two examined strains showed different plasmid profiles; however bands of similar sizes were seen in both profiles. Genes responsible for salivaricin production are located on chromosomal DNA. Properties of these strains, in particular the presence of genes encoding bacteriocin production, imply that they may be used as potential probiotics for poultry.

13

Comparative analysis of the gene encoding RNA-dependent RNA polymerase in RHD virus strains originating from various continents

72%

Hukowska-Szematowicz B., Lejkowska R., Deptula W.

Advances in Agricultural Sciences

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2010

|

tom 13

|

nr 1

14

Identification of novel genes potentially involved in rice (Oryza sativa L.) drought tolerance

72%

Zinati Z.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2017

|

tom 98

|

nr 3

15

Consequences of mitochondrial translation perturbation for the whole cell transcriptome

72%

Adamowicz A., Kwasniak M., Janska H.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

16

What roles for hydrogen peroxide (H2O2) in the legume – Rhizobium symbiosis?

72%

Andrio E., Gucciardo S., Mandon K., Marino D., Oger E., Puppo A., Pauly N.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 2

17

Transcriptome and proteome changes accompanying increased vigor of osmoprimed rape (Brassica napus L.) seeds

72%

Kubala S., Lechowska K., Wojtyla L., Kosmala A., Quinet M., Lutts S., Garnczarska M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

18

The subcellular localization of UVR3 and two putative photolyases, PHR2 and At4g25290

72%

Aggarwal C., Labuz J., Sztatelman O., Banas A. K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 3

19

Short-term heat stress effects on a cyp 11A1 canola plants

72%

Sakhno L., Slyvets M., Korol N., Karbovska N., Ostapchuk A., Kuchuk M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

20

Comparative analysis of the gene encoding VP60 capsid protein in various strains of the RHD (rabbit haemorrhagic disease) virus

72%

Hukowska-Szematowicz B., Manelska A., Tokarz-Deptula B., Deptula W.

Advances in Agricultural Sciences

|

2011

|

tom 14

|

nr 1-2

Ograniczanie wyników

Spontaneous mutagenesis in exponentially growing and stationary-phase, umuDC-proficient and -deficient, Escherichia coli dnaQ49

Altering substrate specificity of catechol 2,3-dioxygenase from Planococcus sp. strain S5 by random mutagenesis

A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

Lack of salidroside impact on selected cytochromes encoding genes transcription in the liver of ethanol induced rats

Cucumber HMA5A and HMA5B are two tonoplast-localized homologs of Arabidopsis thaliana heavy metal ATPase HMA5 involved in cellular heavy metal homeostasis

Thermostable Pyrococcus woesei beta-D-galactosidase - high level expression, purification and biochemical properties

Cloning and characterization of the gene encoding ribosomal PO phosphoprotein from Neurospora crassa

Identification and characterization of two XTH related genes involved in cucumber [Cucumis sativus L.] somatic embryogenesis

Development of a multiplex PCR [m-PCR] test for rapid identification of genes encoding heat-labile [LTI] and heat-stable [STI and STII] toxins of enterotoxigenic Escherichia coli [ETEC] with internal control of amplification