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In rats exposed for 28 days (5 hours a day) to ozone at a concentration of 0.5 ppm and receiving alpha-tocopherol at doses of 4.5 mg/rat and 15 mg/rat, levels of acute phase proteins (APP) - C-reactive protein (CRP), ceruloplasmin (Cp), total protein, gamma-globulins, and activity of lysozyme in blood serum were studied. The assays were performed in the presence of respective control groups, i.e. rats receiving the same doses of alpha-tocopherol but not exposed to ozone, a group of animals not supplemented with vitamin but exposed to ozone, a group of animals injected with physiological fluid and a control group not subjected to any of the treatments. The study revealed that the ozone-exposed animals had an increased lysozyme activity and a decreased total protein level. However, in rats protected by alpha-tocopherol and exposed to ozone, the concentration of APP, lysozyme activity and total protein were found to be decreased. Similar relationships also occurred in animals receiving alpha-tocopherol and not exposed to ozone.
Immersing Siberian sturgeon fry of an average body mass of 40 ± 4 g in epin solution (0.1 mg⁻¹) resulted in an increase of ceruloplasmin level over the entire experimental period (P < 0.001). The differences between the treated and control groups gradually increased reaching the maximum in the fourth and fifth weeks of the study when average levels of ceruloplasmin in the epin-treated group were 2.5 and 5.7-fold higher, respectively. In the control group, ceruloplasmin levels were considerably higher in the first four weeks in comparison to the last two weeks (P < 0.01). The bacteriolytic activity of lysozyme increased up to 50% compared to the control group (P < 0.05) in the first and fourth weeks. The sturgeon in the epin-treated group also showed from 10 to 65% higher concentrations of γ-globulin levels than the control fish, and in the second week the difference was statistically significant (P< 0.01). Simultaneously, total protein concentrations also increased (P< 0.05).
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