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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Mutations modulate soluble carbohydrates composition in seeds of Lathyrus sativus L.

100%
Piotrowicz-Cieslak A. I., Rybinski W., Michalczyk D. J.
Acta Societatis Botanicorum Poloniae
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2008
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tom 77
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nr 4
281-287
Seeds of Lathyrus sativus cv. Derek and Krab were used as biological material for induced mutagenesis. Three mutant lines were obtained from seeds of grass pea cv. Derek and 15 lines from mutagenised seeds of cv. Krab. Twelve ethanol-soluble carbohydrates were identified in the seeds. We have selected grass pea mutant lines with high oligosaccharides content (lines D4, K56, K25, and K7) and lines with low raffinose family oligosaccharides (RFO) content (lines K12, K29 and K13). Mutations changing the levels of RFO have not affected the contents of galactosyl cyclitols.
2

Accumulation of raffinose family oligosaccharides and galactosyl cyclitols in developing Vicia seeds

100%
Lahuta L. B., Gorecki R. J., Gojlo E., Horbowicz M.
Polish Journal of Natural Sciences. Supplement
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2003
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tom 01
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The expression profile of galactinol synthase genes (VhGolS1 and VhGolS2) in developing Vicia hirsuta ([L.] S.F. Gray) seeds

84%
Podlinski P., Gojlo E., Pupel P., Jastrzebski J. P., Paukszto L., Gorecki R. J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 3
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Effect of exogenous abscisic acid on accumulation of raffinose family oligosaccharides and galactosyl cyclitols in tiny vetch seeds [Vicia hirsuta [L.] S.F.Gray]

84%
Lahuta L. B., Gorecki R. J., Gojlo E., Horbowicz M.
Acta Societatis Botanicorum Poloniae
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2004
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tom 73
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nr 4
The role of the abscisic acid (ABA) in biosynthesis of raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in tiny vetch (Vicia hirsuta [L.] S.F. Gray) seeds was investigated. The ABA was applied through incubation of seed at various stage of its development. The level of RFOs and Gal-C was determined in seed maturing on plant and in seed maturing in vitro. In early stages of V. hirsuta seed development, the ABA activated the biosynthesis of galactinol, although the level of arisen galactinol quickly declined. In the later stages of V. hirsuta seed development ABA had stimulatory effect of RFOs and Gal-C biosynthesis. Influence of ABA on biosynthesis of a-galactosides in Vicia hirsuta seed seems to be dependent on abscisic acid concentration. Low concentration of ABA had stimulatory effect on a-galactosides biosynthesis, but high concentration of ABA inhibited the process.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Cyclitols, galactosyl cyclitols and raffinose family oligosaccharides in Mexican wild lupin seeds

84%
Piotrowicz-Cieslak A. I., Lopez P. M. G., Gulewicz K.
Acta Societatis Botanicorum Poloniae
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2003
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tom 72
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nr 2
Ten to 16 ethanol-soluble carbohydrate components were identified in the seeds of six Mexican wild lupins. The analysed carbohydrates included: monosaccharides, disaccharides, cyclitols, galactosyl cyclitols and raffinose family oligosaccharides. Stachyose and sucrose were the main carbohydrate component in the Lupinus montanus, L. rotundiflorus, L. exaltatus, L. mexicanus and L. elegans seeds. Only trace quantities of verbascose were detected in Lupinus mexicanus seeds. The analysed seeds accumulated 38 to 78 mg/g d.m. carbohydrates. The raffinose family oligosaccharides constituted 41 to 85.2% of the identified carbohydrate component pool. The analysed Lupinus seeds contained 3 to 8 unidentified carbohydrate components.
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Different effects of soil drought on soluble carbohydrates of developing Lupinus pilosus and Lupinus luteus embryos

67%
Piotrowicz-Cieslak A. I., Michalczyk D. J., Adomas B., Gorecki R. J.
Acta Societatis Botanicorum Poloniae
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2007
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tom 76
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nr 2
The aim of this study was to compare the accumulation of soluble carbohydrates in embryos of two lupin species: cultivated Lupinus luteus (cv. Juno) and wild L. pilosus, developing on plants grown under normal soil humidity and soil drought. All analysed seeds accumulated soluble carbohydrates, including: monosaccharides, sucrose, cyclitols, galactosyl cyclitols and raffinose family oligosaccharides. Soil drought caused a nearly two-fold increase of soluble carbohydrate contents in both species. L. pilosus embryos however, responded to water deficiency by increasing the accumulation of cyclitols and galactosyl cyclitols, whereas L. luteus embryos enhanced accumulation of cyclitols and raffinose family oligosaccharides.
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Composition of seed soluble carbohydrates and ultrastructural diversity of testa in lupins from the Mediterranean Region

67%
Piotrowicz-Cieslak A. I.
Acta Societatis Botanicorum Poloniae
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2005
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tom 74
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nr 4
This article present a comparison of soluble sugar levels in seeds of Lupinus atlanticus, Lupinus cosentinii, Lupinus palaestinus and Lupinus pilosus, Lupinus hispanicus subsp. hispanicus and Lupinus luteus of Juno variety. Considering that sugars are accumulated in embryos, only the embryonic tissues were used for biochemical analyses. Additionally, the share of testa and embryo in seed tissues was evaluated. The seed-coat thickness was measured using scanning electron microscopy. The seed coat had the largest share in seeds of Lupinus pilosus and Lupinus palaestinus, and the least share in seeds of Lupinus hispanicus subsp. hispanicus and Lupinus luteus of Juno variety. In the seed of Lupinus pilosus the thickness of the seed coat was 1100 µm, while in Lupinus luteus it was 300 µm. The analysed Lupinus seeds accumulated from 73 mg/g d.m. (dry matter of seed embryo) to 155 mg of soluble sugars/g d.m. The highest quantity of oligosaccharides of the raffinose series was found in seeds of Lupinus luteus, while the lowest amount in seed of Lupinus palaestinus. Galactosyl cyclitols appeared in largest amount in seeds of Lupinus palaestinus and Lupinus pilosus, appropriately 41.93 and 33.75 mg/g dm. The lowest amount of galactosyl cyclitols appeared in Lupinus atlanticus, Lupinus cosentinii and Lupinus hispanicus.
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Carbohydrates in Colobanthus quitensis and Deschampsia antarctica

67%
Piotrowicz-Cieslak A. I., Gielwanowska I., Bochenek A., Loro P., Gorecki R. J.
Acta Societatis Botanicorum Poloniae
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2005
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tom 74
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nr 3
Eight to nineteen ethanol-soluble carbohydrate components were identified in vegetative tissues of Colobanthus quitensis and Deschampsia antarctica. The analysed carbohydrates included: monosaccharides, cyclitols, galactosyl cyclitols, raffinose family oligosaccharides, lichnose family oligosaccharides, kestose family oligosaccharides. The analysed vegetative tissues accumulated from 447 to 139 mg/g d.m. soluble carbohydrates in Colobanthus quitensis, Deschampsia antarctica respectively. The raffinose family oligosaccharides constituted 53.3% in Colobanthus quitensis of the identified soluble carbohydrate component pool. Vegetative tissues accumulated starch in Colobanthus quitensis 20.6 mg/g d.m. and 261.6 mg/g d.m. in Deschampsia antarctica. Anatomical and ultrastructural observations of vegetative part of Colobanthus quitensis and Deschmpsia antarctica revealed the presence of various ergastic materials in intercellular spaces, cell walls and protoplasts. Various parts of these plants contain insoluble, PAS positive polysaccharides in intercellular spaces and in cell walls. Chloroplasts of analysed tissues contained starch. Less starch was visible in young, growing parts of shoots of Colobanthus quitensis and Deschmpsia antarctica, more starch appears in mature, differentiated parts.
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