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The effect of galactinol synthase activity on RFOs content in developing pea seeds (Pisum sativum L.) subjected to drying and cold

100%

Gojlo E., Szymczak M., Kondrat B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

2

The expression profile of galactinol synthase genes (VhGolS1 and VhGolS2) in developing Vicia hirsuta ([L.] S.F. Gray) seeds

100%

Podlinski P., Gojlo E., Pupel P., Jastrzebski J. P., Paukszto L., Gorecki R. J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

3

Analysis of expression profiles of barley, medicago and soybean galactinol synthase through microarray data integration

80%

Paukszto L., Myszczynski K., Gojlo E., Jastrzebski J. P., Pupel P., Podlinski P.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

4

Exogenously applied D-pinitol and D-chiro-inositol modifies the accumulation of alpha-D-galactosides in developing tiny vetch [Vicia hirsuta [L.] S.F.Gray] seeds

80%

Lahuta L. B., Horbowicz M., Gojlo E., Goszczynska J., Gorecki R. J.

Acta Societatis Botanicorum Poloniae

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2005

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tom 74

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nr 4

In the present study we have investigated the effect of exogenous cyclitols on the accumulation of their galactosides and raffinose family oligosaccharides (RFOs), as well as on some enzymes important for their biosynthesis in seeds of tiny vetch (Vicia hirsuta [L.] S.F. Gray). Immature seeds during 6-day incubation with D-chiro-inositol (naturally does not appear in seeds of tiny vetch) were accumulated cyclitol and its galactosides (fagopyritols: B1 and B2). Short 4-hour incubation with D-chiro-inositol, and subsequent slow desiccation process caused accumulation of free cyclitol only, without biosynthesis of its galactosides. Feeding D-chiro-inositol to pods of tiny vetch induced accumulation of high levels of its galactosides (fagopyritol B1, B2 and B3) in maturing seeds. Similarly, feeding D-pinitol increased accumulation of its mono-, di- and tri-galactosides: GPA, GPB, DGPA and TGPA in tiny vetch seed. Accumulation of both cyclitols and their galactosides drastically reduced accumulation of verbascose. Inhibition of RFOs biosynthesis by elevated levels of free cyclitols suggests some competition between formation of both types of galactosides and similarity of both biosynthetic routes in tiny vetch seeds. Galactinol synthase (GolS) from tiny vetch seeds demonstrated ability to utilize D-chiro-inositol as galactosyl acceptor, instead of myo-inositol. Presence of both cyclitols, as substrates for GolS, caused synthesis of their galactosides: fagopyritol B1 and galactinol. However, formation of galactinol was more efficient than fagopyritol B1. D-chiro-Inositol and D-pinitol at concentrations several-fold higher than myo-inositol had inhibitory effect on GolS. Thus, we suggest that a level of free cyclitols can have an influence on the rate of galactinol biosynthesis and further accumulation of RFOs and galactosyl cyclitols in tiny vetch seeds.

Ograniczanie wyników

3 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Acta Societatis Botanicorum Poloniae

4 Gojlo E.

2 Gorecki R. J.

2 Jastrzebski J. P.

2 Paukszto L.

2 Podlinski P.

2 Pupel P.

1 Goszczynska J.

1 Horbowicz M.

1 Kondrat B.

1 Lahuta L. B.

1 Myszczynski K.

1 Szymczak M.

3 2013

1 2005

The effect of galactinol synthase activity on RFOs content in developing pea seeds (Pisum sativum L.) subjected to drying and cold

The expression profile of galactinol synthase genes (VhGolS1 and VhGolS2) in developing Vicia hirsuta ([L.] S.F. Gray) seeds

Analysis of expression profiles of barley, medicago and soybean galactinol synthase through microarray data integration

Exogenously applied D-pinitol and D-chiro-inositol modifies the accumulation of alpha-D-galactosides in developing tiny vetch [Vicia hirsuta [L.] S.F.Gray] seeds