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Evaluation of anti-gout activity of some plant food extracts

100%

Mohamed D. A., Al-Okbi S. Y.

Polish Journal of Food and Nutrition Sciences

2008

tom 58

nr 3

The anti-gout activity of methanol and petroleum ether extracts of celery leaves, celery seeds, rosemary, cinnamon and turmeric as functional food components was studied in potassium oxonate treated rats (250 mg/kg body weight, intra-peritoneal). Blood samples were collected from all rats after an overnight fast and after 3 and 6 h from oxonate injection for determination of erythrocyte sedimentation rate (ESR), plasma uric acid, nitric oxide (NO) and malondialdehyde (MDA). Urine samples were collected for 6 h after injection for the determination of uric acid. Assessment of total phenolic contents, fatty acids and unsaponifiable matter (UNSAP) in the plants under study was carried out. Results showed that oxonate treatment produced a significant increase in all studied parameters compared to the healthy rats. Oral administration of different extracts (500 mg/kg body weight) showed a significant reduction in plasma and urine uric acid levels, petroleum ether extract of celery seeds was the most promising. The majority of administered extracts showed significant reduction in inflammatory (ESR and NO) and oxidative stress (MDA) markers with variable degrees. GLC investigation of plants UNSAP revealed the presence of different phytosterols. GLC analysis of the fatty acids methyl ester showed that celery seeds and leaves contained the highest contents of oleic and linoleic acid, respectively. Linolenic acid was only present in celery seeds and leaves. All the studied plants were rich in phenolics; rosemary was superior in this respect. In conclusion, the studied plant extracts showed significantly variable anti-gout activity associated with both antioxidant and antiinflammatory effects, which may be due to the presence of phenolic compounds, unsaturated fatty acids, long chain fatty acids and phytosterols.

A preliminary assessment of singlet oxygen scavenging, cytotoxic and genotoxic properties of Geranium macrorrhizum extracts

67%

Venskutonis P. R., Dedonyte V., Lazutka J., Slapsyte G., Maroziene A., Nemeikaite-Ceniene A., Cenas N., Miliauskas G.

Acta Biochimica Polonica

2010

tom 57

nr 2

Strong radical-scavenging activity of Geranium macrorrhizum extracts isolated by using various solvent systems has been reported previously. This study aimed at expanding the knowledge on the bioactivities of antioxidatively active G. macrorrhizum butanol fraction, which was isolated from ethanolic extract (EB), and water fraction, which was isolated from water extract (WW) by measuring their singlet oxygen scavenging properties, as well as preliminary assessment of cytotoxicity and genotoxicity toward mammalian cells. The cytotoxicity (necrosis induction) of the extracts in bovine leukemia virus-transformed lamb kidney fibroblasts (line FLK) was partly prevented by antioxidants and stimulated by the prooxidant BCNU (N,N'-bis(2-chloroethyl)-N-nitrosourea). This indicates that the cytotoxicity of G. macrorrhizum extracts is at least partly attributed to their prooxidant action, presumably due to the formation of quinoidal products of their (auto)oxidation. The latter was evidenced by the nature of the peroxidase-catalyzed oxidation products, which supported DT-diaphorase-catalyzed oxidation of NADPH and participated in conjugation reactions with reduced glutathione. The genotoxic properties were studied using chromosome aberration (CA) and sister chromatid exchange (SCE) tests in human lymphocytes in vitro and Drosophila melanogaster somatic mutation and recombination test (SMART) in vivo. In the CA test, only the highest doses of both fractions significantly increased chromosome aberration frequency. In the SCE test, both fractions induced SCEs in a clear dose-dependent manner. G. macrorrhizum extracts were not genotoxic in the SMART test in vivo. Our data indicate that in spite of the possible beneficial (antioxidant) effects of Geranium extracts, the possibilities of their use as ingredients of functional foods and/or food supplements should be further examined due to their cyto- and genotoxic effects resulting mainly from the action of quercetin-derived components abundant in the extracts.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Polish Journal of Food and Nutrition Sciences

1 Al-Okbi S. Y.

1 Cenas N.

1 Dedonyte V.

1 Lazutka J.

1 Maroziene A.

1 Miliauskas G.

1 Mohamed D. A.

1 Nemeikaite-Ceniene A.

1 Slapsyte G.

1 Venskutonis P. R.

1 2010

1 2008

Wyniki wyszukiwania

Evaluation of anti-gout activity of some plant food extracts

A preliminary assessment of singlet oxygen scavenging, cytotoxic and genotoxic properties of Geranium macrorrhizum extracts