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1

Functional analysis of gluB gene in Solanum tuberosum plants

100%
Hennig J., Krzymowska M., Barabasz A., Mac A., Bieniak B., Charzynska M.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
2

Sequencing and functional analysis of the yeast genome

86%
Zagulski M., Herbert C. J., Rytka J.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 3
The genome of the yeast Saccharomyces cerevisiae was sequenced by an international consortium of laboratories from Europe, Canada, the U.S.A. and Japan. This project is now finished and the complete sequence of the first eukaryotic genome was released to the public data bases in April 1996. An overview and preliminary analysis of the entire genome sequence was presented in a special issue of Nature in May 1997, entitled "The yeast genome directory". At its origin the Yeast Genome Sequencing Project provoked much debate and controversy; however, the final results obtained and the insights this has given us into the organisation and content of a eukaryotic genome have more than justified the expectations of the supporters of the project. The importance of genomic sequencing and analysis, especially of model organisms, is now widely accepted and this has resulted in the birth of the new science of genomics (Botstein & Cherry, Proc. Natl. Acad. Sci. U.S.A. 94, 5506, 1997). The information from gene and protein sequences ultimately lead to functional description of all genes. The main strategies describing possible ways to analyse the function of new genes that have been identified by systematic sequencing of Saccharomyces cerevisiae genome are described.
3

Two-factorial functional analysis of economic development in the regions of Azerbaijan

72%
Humbatova S. I., Hajiyev N. G.-O.
Electronic Journal of Polish Agricultural Universities. Series Economics
|
2017
|
tom 20
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nr 4
4
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Functional analysis of peroxiredoxin isoforms in Euglena gracilis

72%
Tamaki S., Maruta T., Sawa Y., Shigeoka S., Ishikawa T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
|
tom 94
|
nr 2
5

Eukaryotic microbial communities associated with rock-dwelling foliose lichens: a functional morphological and microecological analysis

72%
Bock N., Anderson O. R.
Acta Protozoologica
|
2015
|
tom 54
|
nr 4
6
Content available remote

Functional analysis of eicosanoids from white blood cells in sepsis and SIRS

72%
Baenkler M., Leykauf M., John S.
Journal of Physiology and Pharmacology. Supplement
|
2006
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tom 57
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nr 12
25-33
Sepsis and SIRS are affections with major alterations in inflammatory activity. The impact of prostaglandins (PG) and leukotrienes (LT) produced from white blood cells (WBC) in this context is not completely understood. Thirty nine patients with sepsis or SIRS were investigated in comparison to 10 healthy controls. WBC were collected and separately exposed to arachidonic acid (AA) or to nothing else. After centrifugation, the generated PGE2 and LTCDE4 with or without stimulation were measured in the supernatant. LT-levels were significantly higher during sepsis/SIRS than in controls whereas PG-levels of patients were decreased to those of controls in basic condition. The relation between the level with and without stimulation showed a significant higher ratio in PG in contrast to LTs. The survivor’s ratio in LT levels was significantly higher than that of non-survivors, which did not differ from controls. Generation of LT from WBC is enhanced during sepsis/SIRS, but LT generation after stimulation only in survivors but not in non-survivors. This inability of WBC to generate LT during sepsis in non-survivors could be predictive regarding the outcome of sepsis/SIRS and may be part of the “immunoparalysis” seen during sepsis in association with bad outcome.
7

Biochemical and functional analyses of a peptide-phospholipid-DNA ternary complex used for gene delivery

72%
Legendre J. Y., Huang S. K., Szoka F. C.
Cellular and Molecular Biology Letters
|
1999
|
tom 04
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nr 2
8

How to assess the pathogenicity of mutations in Charcot-Marie-Tooth disease and other diseases?

72%
Kochanski A.
Journal of Applied Genetics
|
2006
|
tom 47
|
nr 3
Knowledge whether a certain DNA variant is a pathogenic mutation or a harmless polymorphism is a critical issue in medical genetics, in which results of a molecular analysis may serve as a basis for diagnosis and genetic counseling. Due to its genetic heterogeneity expressed at the levels of loci, genes and mutations, Charcot-Marie-Tooth (CMT) disease can serve as a model group of clinically homogenous diseases for studying the pathogenicity of mutations. Close to a 17p11.2-p12 duplication occurring in 70% of patients with the demyelinating form of CMT disease, numerous mutations have been identified in poorly characterized genes coding for proteins of an unknown function. Functional analyses, segregation analyses of large pedigrees, and inclusion of large control groups are required to assess the potential pathogenicity of CMT mutations. Hence, the pathogenicity of numerous CMT mutations remains unclear. Some variants detected in the CMT genes and originally described as pathogenic mutations have been shown to have a polymorphic character. In contrast, polymorphisms initially considered harmless were later reclassified as pathogenic mutations. However, the process of assessing the pathogenicity of mutations, as presented in this study for CMT disorders, is a more general issue concerning all disorders with a genetic background. Since the number of DNA variants is still growing, in the near future geneticists will increasingly have to cope with the problem of pathogenicity of identified genetic variants.
9

Spojrzenie na analize wartosci z perspektywy FMEA

58%
Myszewski J. M.
Problemy Jakości
|
2004
|
tom 36
|
nr 07
14-17
10
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Functional analysis of ABI5 and RGL2 in dormancy breaking of Acer platanoides L. seeds

58%
Staszak A., Guzicka M., Maslowska S., Pawlowski T. A.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 3
11
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Content available

Transcriptome sequencing: next generation approach to RNA functional analysis

58%
Zmienko A., Jackowiak P., Figlerowicz M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2011
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tom 92
|
nr 4
12

A new BSMV-based vector with modified beta molecule allows simultaneous and stable silencing of two genes

58%
Kawalek A., Dmochowska-Boguta M., Nadolska-Orczyk A., Orczyk W.
Cellular and Molecular Biology Letters
|
2012
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tom 17
|
nr 1
Virus-induced gene silencing is an important tool for functional gene analysis and the vector based on Barley stripe mosaic virus (BSMV) is widely used for the purpose in monocots. Of the tripartite BSMV genome, currently the BSMV:γMCS molecule is used to clone a fragment of a target gene. As an alternative, the BSMV:β molecule was engineered with a unique BamHI site between the open reading frame of βc (ORF βc) and poly(A). The mixture of RNA particles α, βBamHI and γMCS was fully infectious. Barley phytoene desaturase and wheat phospholipase Dα fragments were cloned to βBamHI and γMCS. Delivery of the target gene fragment in γMCS induced stronger silencing, while delivery in βBamHI yielded more stable transcript reduction. A quantitative analysis (qRT-PCR) of the transcripts showed that the silencing induced with a fragment carried in both particles was stronger and more stable than that from a fragment placed in one particle. The modification of β enables simultaneous silencing of two genes. Quantifying the β and γ particles in virus-inoculated plants revealed a 2.5-fold higher level of γ than β, while the stability of the insert was higher in β compared with γ. The possible influence of the relative quantity of β and γ particles in virus-inoculated plants on insert stability and gene silencing efficiency is discussed.
13

Agrobacterium rhizogenes-mediated transformation of a high oil-producing filamentous fungus Umbelopsis isabellina

58%
Wei D.-S., Zhang Y.-H., Xing L.-J., Li M.-C.
Journal of Applied Genetics
|
2010
|
tom 51
|
nr 2
225-232
The Agrobacterium rhizogenes-mediated transformation procedure was developed by using the hygromycin B phosphotransferase gene (hph) as a selective marker for the oil-producing fungus Umbelopsis isabellina. Different conditions were combined to increase the transformation efficiency. The highest efficiency was obtained by using A. rhizogenes strain R105 and a vector with zygomycete promoter. Southern blot analysis demonstrated that 71% of transformants contained random integrations of T-DNA sequences under optimal conditions. We randomly selected 115 positive transformants resistant to hygromycin to analyze the amount of total fatty acid and gamma-linolenic acid (GLA). Six transformants produced a higher amount of total fatty acids than the wild strain, and one transformant also produced a higher level of GLA than the wild strain in gas chromatography analysis. This is the first report about using A. rhizogenes strain R105 and germinated conidia to transform successfully the recalcitrant zygomycetes and to obtain transformants with a stable phenotype.
14
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Content available

Target costing calculation in the woodworking industry to support demand at a time of global recession

58%
Potkany M., Hajdukova A., Teplicka K.
Drewno. Prace Naukowe. Doniesienia. Komunikaty
|
2012
|
tom 55
|
nr 187
At a time of global recession, more and more companies are increasingly looking for ways to innovate management systems. The main aim of this paper is to present the practical possibilities of a target costing application within the calculation system of the woodworking industry. This calculation uses the basic principles of value analysis, by which it is possible to suggest alternatives to the functional and cost differentiation of the product, and is the main information for managers in decision-making process.
15

The structural and functional analysis of the human HSPA2 gene promoter region

58%
Piglowski W., Nowak R., Krawczyk Z., Scieglinska D.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 1
HSPA2 is a human counterpart of the testis-specific rodent Hst70/Hsp70.2 gene. In contrast to the latter, the expression of the human HSPA2 gene is not limited to the testis, and recent data show that human tumor cells can express this gene at significant levels. The characteristics of HSPA2 expression suggests that it can influence the phenotype and survival of cancer cells similarly as overexpression of major members of the HSP70 gene family. Until now, neither the structure of the transcription unit of the human HSPA2 gene has been established nor a functional analysis of its promoter performed. In this study we established that the human HSPA2 gene, in contrast to its rodent counterparts, is intronless and has a single transcription start site. We also show that the same type of HSPA2 transcripts are synthesized in the testes and in cancer cell lines. In order to perform a functional study of the HSPA2 promoter, we used a transient transfection assay and found that the 392 bp fragment upstream of the ATG codon was a minimal region required for efficient transcription, while a 150 bp deletion from the 5' end of this region dramatically reduced the promoter activity. Delineation of the minimal promoter is a basic step toward identifiying the cis and trans elements involved in the regulation of the HSPA2 gene expression in cancer cells.
16

Functional analysis of a basic chitinase in Arabidopsis thaliana using reverse genetics

58%
Rodakowska E., Wojtaszek P.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
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