Wyniki wyszukiwania - AGRO

1

Concentration changes in the emission anisotropy of FMN in PVA films

100%

Zurkowska G., Grajek H., Bojarski P., Bojarski C.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

2

Continuous recording of intramitochondrial pH with fluorescent pH indicators: novel probes and limitations of the method

80%

Zolkiewska A., Czyz A., Duszynski J., Wojtczak L.

Acta Biochimica Polonica

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1993

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tom 40

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nr 2

In addition to 2',7'-bis-(2-carboxyethyl)-5(6)- carboxyfluorescein (BCECF) used so far to monitor intramitochondrial pH, two other fluorescent pH indicators, 4',5'-dimethyl-5(6)-carboxyfluorescein (DMCF) and carboxyseminaphthofluorescein (carboxy-SNAFL-1), were applied for this purpose. These probes are taken up by isolated rat liver mitochondria in form of diacetate esters, hydrolyzed within mitochondria to free acids, and respond to changes of intramitochondrial pH by changing their fluorescence emission intensity. With all three probes energization of mitochondria by electron donors or acceptors was accompanied by fluorescence changes characteristic for alkalization, whereas deenergization by respiratory inhibitors or protonophores produced changes typical for acidification. Contrary to this, transition from State 4 to State 3, known to shift intramitochondrial pH towards acidification (equivalent to a decrease of ApH), was accompanied by paradoxical responses of the fluorescent pH probes used: the fluorescence of DMCF increased as if the matrix compartment became more alkaline, the fluorescence of BCECF, measured in single excitation/emission wavelength mode, did not change, and the fluorescence of carboxy-SNAFL-1 could be interpreted as either alkalization or acidification, depending on the excitation/emission wavelength pair used. It was shown that depletion of intramitochondrial Mg2+ and Ca2+ using divalent metal ionophore A23187 decreased fluorescence intensity with all three probes examined, whereas subsequent addition of Mg2+ or Ca2+ increased the fluorescence. It is therefore proposed that the atypical response of intramitochondrial pH indicators upon State 4 - State 3 transition is due to changes of intramitochondrial free Mg2+, as related to different complexing abilities of ATP and ADP towards magnesium.

3

Properties of DOTAP-containing liposomes - fluorescence studies

80%

Olzynska A., Jurkiewicz P., Langner M., Hof M.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.

4

Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes

70%

Kedrak-Jablonska A., Budniak S., Szczawinska A., Reksa M., Krupa M., Szulowski K.

Bulletin of the Veterinary Institute in Puławy

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2015

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tom 59

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nr 4

The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seeligeri were used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of the tests. QuantiTect SYBR Green PCR and QuantiTect Probe PCR kits were selected for the study. In the first stage of the study, SYBR Green I real-time PCRs were performed under several methods, the first one allowing detection of the 23S rDNA gene and the remainder based on the amplification of the hlyA gene. In the next part, three varied in method TaqMan probe-based real-time PCRs allowing confirmation of strains belonging to Listeria spp. and L. monocytogenes were conducted. The observation of amplification curves in real-time PCR methods enabled the detection of both genes, and these methods demonstrated a significant sensitivity and high specificity. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA and hlyA genes, which confirmed the tested strains as Listeria spp. and L. monocytogenes respectively. Isolates of other microbial species did not yield real-time PCR products.

5

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1;25 centric fusion recognition in the cattle chromosome by fluorescence in situ hybridization

70%

Ladon D., Solinas-Toldo S., Fries R., Stranzinger G. F.

Journal of Applied Genetics

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1996

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tom 37

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nr 1

Fluorescence in situ hybridization (FISH) experiments with specific probes for chromosome 29 and 25 were carried out on a Brown Swiss bull, previously diagnosed as a carrier of 1;29 centric fusion. The hybridization of the chromosome 29-specific probe (BMC 4216 - already located on 29q13), produced signals on two small acrocentrics, but not on the translocated chromosome. The signals appeared on the translocated chromosome and on a single chromosome 25 after hybridization of the chromosome-specific probe (BMC 3224 - previously located on 25q24). According to the actual nomenclature, the analysed aberration is a robertsonian translocation involving chromosomes 1 and 25.

6

Application of luminescence for quality evaluation of in vitro regenerated strawberry plants

70%

Murkowski A., Bartoszewski G., Skorska E.

Acta Physiologiae Plantarum

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1998

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tom 20

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nr 4

The parameters of chlorophyll a fluorescence induction were measured: Fv/Fm, Sc/Fm, Rfd and coefficient of Ld delayed luminescence decay kinetics, related with a course of primary photosynthesis reactions on leaves of strawberry plants, cultured in vitro by means of the micropropagation methods. Strawberry plants cv. Ananasowa from in vitro cultures in optimal condition show significantly higher values of luminescence parameters indicating better condition of plants of this variety in comparison with the variety Senga Sengana. After temperature lowering, however, these values were more reduced than for plants of Senga Sengana, which can be interpreted as higher susceptibility of this variety to chill. Addition of BAP caused disturbance of primary photosynthesis reactions rate, particularly in lower temperature. Auxin 2,4-D had no effect on the luminescence parameters in comparison with control cultures. Dehydration stress strongly diminished the values of measured parameters for Ananasowa variety what indicates the inhibition of primary photosynthesis reaction in leaves. The old culture of Senga Sengana variety showed higher tolerance on linuron in comparison with the new one.

7

A comparative CD and fluorescence study of a series of model calcium-binding peptides

70%

Goch G., Kozlowska H., Wojtowicz A., Bierzynski A.

Acta Biochimica Polonica

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1999

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tom 46

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nr 3

Lanthanide-saturated peptides analogous to calcium-binding loops of EF-hand proteins can be used to stabilize the α-helical structure of peptide or protein segments attached to their C-termini. To study conformational properties of such loop-containing hybrids it is necessary to produce them in bacteria. In peptides obtained in this way the helix will be destabilized by the negatively charged C-terminal α-carboxyl groups. We propose to blocke them by the homoserine lactone. The results presented in this paper indicate that the presence of the lactone even at the C-terminus of the loop does not have any negative effect on the loop helix-nucleation ability. On the other hand, the presence of the α-NH+3 at the loop N-terminus leads to a drop of metal-binding constant and loss of the rigid structure of the α-helical segment of the loop. The α-amino group separated by one glycine residue from the loop N-terminus should also be avoided because it perturbs the conformation of the N-terminal part of the loop and may reduce the loop affinity to lanthanide ions.

8

The interaction of tryptophan and ANS with PAMAM dendrimers

61%

Klajnert B., Bryszewska M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 4

Dendrimers are globular, hyperbranched polymers possessing a high concentration of surface functional groups and internal cavities. These unique features make them very useful in many biomedical applications, especially as carrier molecules. In this study, the interaction of tryptophan and 1-anilinonaphthalene-8-sulfonic acid with three types of polyamidoamine dendrimers was examined. It was observed that the type of dendrimer surface group has a strong impact on the interactions between the dendrimers and fluorescent molecules.

61%

Malicka J., Groth M., Czaplewski C., Liwo A., Wiczk W.

Acta Biochimica Polonica

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1999

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tom 46

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nr 3

The lifetime distribution calculations were applied to study the influence of configuration of amino-acid residues in positions 2 and 3 on changes in conformation of the peptide chain of cyclic analogues of enkephalins containing a fluorescence energy donor and acceptor in different solvents. In all the solvents studied the lifetime distributions were bimodal. This testified to the presence of two families of conformations. In this paper the relationship between the population of each conformation and configuration of the residues in position 2 and 3, and the solvent used is discussed.

18

Fluorescence in situ hybridization [fish] in uncut plant cells using oligonucleotide DNA probes

61%

Smolinski D. J., Wrobel B.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

19

Short, 12 mer fluorescently labeled methylphosphonated oligonucleotides to visualize beta-actin mRNA in vivo

61%

Lazowski K. W., Kaczmarek L.

Journal of Physiology and Pharmacology

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2003

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tom 54

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nr 4

A pair of fluorescently labeled antisense ( complementary to ß-actin mRNA) or control methylphosphonated DNA 12-mers were introduced into live cells. After fixation their distribution throughout the cell was compared to the localization pattern for the pair of control oligos.The distribution of the two sets of oligos differed in that there was a distinct pool of antisense probes that were detected at elevated levels in the leading edge of fibroblast and cortical underlining. The resulting fluorescence patterns of antisense probes colocalized and were analogous to labeling pattern already described and produced by in situ hybridization . The length of each of the probe destabilized binding to mismatched sequences at physiological temperature, while the overall length of the pair gave a unique, highly sequence specific recognition of a target sequence. Simultaneous, in vivo application of multiple probes let include internal controls into the experimental setup, in order to distinguish different distributions of antisense and control probes in the same specimen.

20

Incorporation of stilbazolium merocyanines into biological membranes

61%

Niedbalska M., Gruda I.

Current Topics in Biophysics

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1992

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tom 16

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nr 1

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