Wyniki wyszukiwania

1

Fibronectin adsorption and cell adhesion

100%

Kowalczynska H. M., Nowak-Wyrzykowska M., Dobkowski J., Kolos R., Kaminski J., Makowska-Cynka A., Marciniak E.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Synthesis and macromolecular organization of gastrointestinal mucin

88%

Slomiany A., Slomiany B. L.

Journal of Physiology and Pharmacology

|

1992

|

tom 43

|

nr 2

Mucus glycoproteins (mucins), the principal determinants of mucus protective qualities and mucosal defense, are studied extensively to define pathological aberrations in the relation to gastrointestinal disease and to develop the mucous barrier strengthening agents. Recent work from our laboratory provided evidence as to the initial stages of the gastrointestinal mucin synthesis, molecular size of the apomucin, its macromolecular organization and interaction with other elements of gastrointestinal mucus. Using monoclonal antibodies against apomucin (clone 1H7), O- glycosylated with N-acetylgalactosamine apomucin (clone 2B4), and that against carboxyl terminal of the apomucin (clone 3G12), the mucin synthesizing polysomes were isolated and glycosylated peptides ranging in size from 6-60kDa identified. The in vitro synthesis in the cell-free system also afforded 60-64kDa products recognized by 1H7 and 3G12 antimucin MAbs. The obtained results provided evidence that the mucin core consists of 60kDa peptide which at cotranslational stage is O-glycosylated with N-acetylgalactosamine. Studies on mucin polymer assembly revealed that mucin preparations prepared by equilibrium density gradient centrifugation and Sepharose 2B chromatography (Mantle, M., Mantle, D., and Allen, A. (1981) Biochem. J. 195, 277-285) are not completely purified and contain DNA and extraneous proteins. The evidence was obtained that so called mucin “link protein”, 118kDa glycopeptide, is a N-glycosylated fragment of fibronectin, whereas the supposedly native undegraded mucin isolated by Carlstedt et al. (Biochem. J. (1983) 211, 13-22) was found to contain mucin-fibronectin-DNA complexes. The general picture that emerged from the studies is that the pure mucin consists of 60kDa glycosylated peptides only. The carboxyl terminal (8-12kDa fragment) of these peptides is not glycosylated (naked) and is responsible for mucin interaction with fibronectin and other fibronectin-like extracellular matrix proteins. While the formation of the mucosal coat depends on many other factors and extracellular components, our findings on mucin structure and interaction with the extracellular matrix proteins provide explanation as to the possible mechanism of mucin adherence to the epithelial surfaces.

3

One session of exercise or endurance training does not influence serum levels of irisin in rats

75%

Czarkowska-Paczek B., Zendzian-Piotrowska M., Gala K., Sobol M., Paczek L.

Journal of Physiology and Pharmacology

|

2014

|

tom 65

|

nr 3

4

Fibronectin fragments in human seminal plasma

75%

Katnik-Prastowska I., Przybysz M., Chelmonska-Soyta A.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 2

The study has revealed the presence of fibronectin (FN) fragments and a lack of intact FN in 72 seminal plasma samples. The FN fragmentation was examined by immunoblotting with a monoclonal antibody specific to the central cellular FN domain and was confirmed with a monoclonal antibody directed to the C-terminal domain of FN. Nine FN fragments between 60 and 200 kDa and five fragments of 60–150 kDa were identified in seminal plasma samples of normozoospermic and of terato-, oligoterato-, and oligoasthenoterato-spermic groups, respectively. The relative amounts of the 60, 90 and 100 kDa FN fragments were 2–3 times higher in seminal plasmas with abnormal semen characteristics than in the normozoospermic group. The results suggest that seminal plasma FN fragments may contribute to fertilization and the analysis of FN fragmentation may have a diagnostic value in andrological investigations.

5

Insulin-like growth factor-I increases laminin, integrin subunits and metalloprotease ADAM12 in mouse myoblasts

63%

Grzelkowska-Kowalczyk K., Grabiec K., Tokarska J., Gajewska M., Blaszczyk M., Milewska M.

Folia Biologica

|

2015

|

tom 63

|

nr 4

6

Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

63%

Pupek M., Lemanska-Perek A., Jasonek J., Katnik-Prastowska I.

Acta Biochimica Polonica

|

2010

|

tom 57

|

nr 3

Three monoclonal antibodies specific to the central cell-binding and the C- and N-terminal domains of fibronectin (FN) were used to test antigenic epitope accessibility on human plasma and cerebrospinal fibronectins. In the plasma group, the mean N-terminal FN domain immunoreactivity was about one fourth that of the cell-binding and C-terminal domains, whereas in cerebrospinal fluid they were nearly equal. In the presence of 0.5-6 M urea N-terminal domain immunoreactivity in the plasma increased 3-6-fold, but it decreased 0.7-3-fold in the cerebrospinal fluid. Analysis of fibronectin domain immunoreactivities of the cell-binding and N-terminal domains by a panel of specific monoclonal antibodies may reveal N-terminal fibronectin domain accessibility for reaction with biological partner ligand(s) and/or processes in which FN could be implicated. Such determinations may have important clinical implications.

7

Reliability of histopathological examination and immunohistochemistry of a single biopsy for evaluation of endometrial health in Icelandic mares

63%

Sikora M., Nowak M., Racheniuk H., Wojtysiak K., Kozdrowski R.

Folia Histochemica et Cytobiologica

|

2017

|

tom 55

|

nr 3

8

The influence of long-lasting implantation on the function of phagocytes towards staphylococci

63%

Rozalska B., Burow A., Ljungh A., Rudnicka W.

Acta Microbiologica Polonica

|

1995

|

tom 44

|

nr 3-4

9

The effect of TGF-beta1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells

63%

Xu G. P., Li Q. Q., Cao X. X., Chen Q., Zhao Z. H., Diao Z. Q., Xu Z. D.

Cellular and Molecular Biology Letters

|

2007

|

tom 12

|

nr 3

The aim of this study was to investigate whether transforming growth factor-β1 (TGF-β1) could induce alveolar epithelial-mesenchymal transition (EMT) in vitro, and whether Smad7 gene transfer could block this transition. We also aimed to elucidate the possible mechanisms of these processes. The Smad7 gene was transfected to the rat type II alveolar epithelial cell line (RLE-6TN). Expression of the EMT-associated markers was assayed by Western Blot and Real-time PCR. Morphological alterations were examined via phase-contrast microscope and fluorescence microscope, while ultrastructural changes were examined via electron microscope. TGF-β1 treatment induced a fibrotic phenotype of RLE-6TN with increased expression of fibronectin (FN), α-smooth muscle actin (α-SMA) and vimentin, and decreased expression of E-cadherin (E-cad) and cytokeratin19 (CK19). After transfecting the RLE-6TN with the Smad7 gene, the expression of the mesenchymal markers was downregulated while that of the epithelial markers was upregulated. TGF-β1 treatment for 48 h resulted in the separation of RLE-6TN from one another and a change into elongated, myofibroblast-like cells. After the RLE-6TN had been transfected with the Smad7 gene, TGF-β1 treatment had no effect on the morphology of the RLE-6TN. TGF-β1 treatment for 48 h resulted in an abundant expression of α-SMA in the RLE-6TN. If the RLE-6TN were transfected with the Smad7 gene, TGF-β1 treatment for 48 h could only induce a low level of α-SMA expression. Furthermore, TGF-β1 treatment for 12 h resulted in the degeneration and swelling of the osmiophilic multilamellar bodies, which were the markers of type II alveolar epithelial cells. TGF-β1 can induce alveolar epithelialmesenchymal transition in vitro, which is dependent on the Smads signaling pathway to a certain extent. Overexpression of the Smad7 gene can partially block this process.

10

Ehrlich ascites tumour cell spreading is induced upon contact with immobilized isolated fibroblast plasma membranes and fibronectin

63%

Wojciak B.

Folia Histochemica et Cytobiologica

|

1993

|

tom 31

|

nr 1

11

Signal transduction in confluent C3H 10T1-2 cells. The role of focal adhesion kinase

63%

Miloszewska J., Trembacz H., Janik P.

Acta Biochimica Polonica

|

2001

|

tom 48

|

nr 1

The activities of extracellular signal-regulated kinases (ERK1/ERK2) is required for proliferation of several types of cells. The performed analysis showed stimulation of ERK's by fetal calf serum (FCS) or fibronectin in the C3H 10T1/2 cell cultures at logarithmic phase of growth. The ERKs activity was not stimulated in confluent cells. This could not be accounted for a partial down regulation of ERK since its level was stable in both types of cells regardless of their density and kind of stimulation. Searching for ERK upstream elements we studied the integrin receptor gene transcript by RT-PCR and focal adhesion kinase (FAK) by Western blotting and phosphorylation assays. It was found that FCS and fibronectin stimulated phosphorylating activity of FAK in the cells at the logarithmic phase of growth, but were inefficient in the confluent cells. RT-PCR showed the presence of α5 and β1 integrin transcripts, and p125FAK was at the same level regardless of the type of stimulation. These data indicate that the ability of FAK to be activated plays an important role in ERK regulation and, in consequence in proliferation and growth inhibition during confluence.

12

Fibronectin and fibrinogen degradation products stimulate PMN-leukocyte and mast cell degranulation

63%

Wojtecka-Lukasik E., Maslinski S.

Journal of Physiology and Pharmacology

|

1992

|

tom 43

|

nr 2

The ability of various peptides cleaved by plasmin from human fibrinogen and fibronectin or fibrinogen- and fibronectin- related synthetic peptides to induce histamine release from mast cells and collagenase and elastase from PMN- leukocytes was examined. Low molecular weight fibrinogen degradation products showed dose dependent secretion of collagenase. These peptides (mol. wt. 1.4 kD) at the concentration of 10⁻⁵ M released about 47% of collagenase and 13% of elastase. Synthetic fib- rinopeptides A and В had a similar strong collagenase releasing potency and also released histamine from mast cells. Peptides from plasmin digestion of fibronectin containing cell attachment site with sequence Arg-Gly-Asp-Ser and also synthetic peptide reproducing this aminoacid sequence at the concentration of 1000 µg/ml released about 50% of collagenase and 55% of elastase from PMN-leukocytes. Moreover peptides containing cell attachment and gelatin binding site induced histamine release from mast cells. The association of fibrinogen and fibronectin degradation with activation of mast cells may motivate the treatment with antihistaminic drugs of all pathological conditions where the intensive protein degradation takes place.

13

Strong association between fibronectin accumulation and lowered cathepsin B activity in glomeruli of diabetic rats

51%

Wyczalkowska-Tomasik A., Bartolomiejczyk I., Gornicka B., Paczek L.

Journal of Physiology and Pharmacology

|

2012

|

tom 63

|

nr 5

14

Desmosealin and other components of the epidermal extracellular matrix

51%

Sandjeu Y., Haftek M.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 4

15

Collagen mRNA and fibronectin are increased in healing gastric ulcers in man

51%

Gillessen A., Shahin M., Pohle T., Foerster E., Domschke W.

Journal of Physiology and Pharmacology

|

1995

|

tom 46

|

nr 1

16

Lymphocyte interactions with extracellular matrix proteins and endothelium in renal allograft recipients

51%

Nowaczyk M., Gorski A., Korczak-Kowalska G., Wierzbicki P., Wyzgal J., Durlik M., Orlowska A.

Archivum Immunologiae et Therapiae Experimentalis

|

1998

|

tom 46

|

nr 1

17

In vitro and in vivo analyses of the biological activity of RGD peptides towards Ab Bomirski melanoma

51%

Okroj M., Dobrzanska-Paprocka Z., Rolka K., Bigda J.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 4

The RGD sequence is present in many extracellular matrix proteins and intracellular proteins, including caspases. Synthetic RGD peptides may affect adhesion, migration and tumour metastasis, or directly induce apoptosis. Several RGD peptides were synthesised, and their anti-adhesive and cytotoxic properties were analysed in vitro. The most active peptide (poly RGD) was also tested in vivo to assess its modulatory activity on melanoma growth. Synthetic RGD peptides inhibit the adhesion of Ab melanoma cells to fibronectin. Poly RGD significantly inhibits primary tumour growth. There was no observed cytotoxicity of poly RGD towards Ab cells in a medium with 10% serum; however, under the same conditions, the anti-adhesive effect of poly RGD was still visible. Experiments on Jurkat cells indicated a weak cytotoxicity of poly RGD and a significant cytotoxicity of GRGDNP (the reference cytotoxic peptide), retained only under serum-free conditions. The anti-tumour effect of poly RGD observed in the Ab Bomirski melanoma model is probably due to an anti-adhesive mechanism. The proapoptotic activity of RGD peptides is dependent on the absence of serum.

18

Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation

51%

Litynska A., Przybylo M., Pochec E., Laidler P.

Acta Biochimica Polonica

|

2002

|

tom 49

|

nr 3

Integrin subunits present on human bladder cells displayed heterogeneous functional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the a2, a5 and β1 integrin sub- units inhibited adhesion of the non-malignant cells, indicating these integrin participation in the adhesion to ECM proteins. In contrast, adhesion of cancer cells was not inhibited by specific antibodies to the β1 integrin subunit. Antibodies to a3 integrin increased adhesion of cancer cells to collagen, LN and FN, but also of the HCV29 line with colagen. It seems that a3 subunit plays a major role in modulation of other integrin receptors especially in cancer cells. Differences in adhesion to ECM proteins between the non-malignant and cancer cell lines in response to Gal and Fuc were not evident, except for the v-raf transfected cell line which showed a distinct about 6-fold increased adhesion to LN on addition of both saccharides. .-Acetylneuraminic acid inhibited adhesion of all cell lines to LN and FN irrespective of their malignancy.

19

Elastin receptor and cell-matrix interactions in heart transplant-associated arteriosclerosis

51%

Hinek A.

Archivum Immunologiae et Therapiae Experimentalis

|

1997

|

tom 45

|

nr 1

20

Antioxidants with carcinostatic activity (resveratrol, vitamin E and selenium) in modulation of blood platelet adhesion

51%

Zbikowska H. M., Olas B.

Journal of Physiology and Pharmacology

|

2000

|

tom 51

|

nr 3

Ograniczanie wyników

Fibronectin adsorption and cell adhesion

Synthesis and macromolecular organization of gastrointestinal mucin

One session of exercise or endurance training does not influence serum levels of irisin in rats

Fibronectin fragments in human seminal plasma

Insulin-like growth factor-I increases laminin, integrin subunits and metalloprotease ADAM12 in mouse myoblasts

Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

Reliability of histopathological examination and immunohistochemistry of a single biopsy for evaluation of endometrial health in Icelandic mares

The influence of long-lasting implantation on the function of phagocytes towards staphylococci

The effect of TGF-beta1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells

Ehrlich ascites tumour cell spreading is induced upon contact with immobilized isolated fibroblast plasma membranes and fibronectin

Signal transduction in confluent C3H 10T1-2 cells. The role of focal adhesion kinase

Fibronectin and fibrinogen degradation products stimulate PMN-leukocyte and mast cell degranulation

Strong association between fibronectin accumulation and lowered cathepsin B activity in glomeruli of diabetic rats

Desmosealin and other components of the epidermal extracellular matrix

Collagen mRNA and fibronectin are increased in healing gastric ulcers in man

Lymphocyte interactions with extracellular matrix proteins and endothelium in renal allograft recipients

In vitro and in vivo analyses of the biological activity of RGD peptides towards Ab Bomirski melanoma

Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation

Elastin receptor and cell-matrix interactions in heart transplant-associated arteriosclerosis

Antioxidants with carcinostatic activity (resveratrol, vitamin E and selenium) in modulation of blood platelet adhesion