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Ceratopteris thalictroides is a water fern very sensitive to both dehydration and low temperature. This study focuses on the cryopreservation of this species by encapsulation-dehydration technique, in particular on the effects of pre-culture step, alginate bead size and the physical conditions of culture on the cryopreservation efficiency. Encapsulated and non-pre-cultured gametophytes did not survive cooling with liquid nitrogen. When cryopreservation was preceded by a 2-week period of pre-culture, regrowth reached 42.1%. Reduction in the size of the alginate bead, and culture in total darkness resulted in improved gametophyte regrowth capacity (75.5% or 81.7%, respectively). The best results (91.3%) were obtained when all factors tested occurred simultaneously. The gametophytes recovered very quickly and sporophytes were formed within 4 weeks after rewarming. These simple improvements can be used, not only for the cryopreservation of gametophytes in cryptogams but also for some recalcitrant species of seed plants.
Azolla filiculoides has been an ephemeral plant in Poland since the end of the 20th century. In the last 15 years this species appeared in 5 locations in south-west Poland. Habitat and plants of two populations became destroyed, three other still exist. A. filiculoides occurs in eutrophic or even polluted water where it forms dense mats, up to 10 cm thick. It stays sterile and propagates only in a vegetative manner. Frost resistance of Lower Silesia populations is higher than reported so far; fern may winter and rebuild the population after frost reaching 22oC. Size of the populations is changeable during the vegetation season. A. filiculoides occurs in water habitats and plant communities in which it substitutes Lemna minor.
Azolla filiculoides Lam. is the second most widely distributed alien aquatic plant in Europe. A native of America, it has become — on account of human mediation — a cosmopolitan species. In Poland, it has been known so far from a few stands in ponds and old river beds. In 2016, it was recorded at six new sites in-between the groynes at the main Oder river bed between Maszewko near Krosno Odrzańskie (52°3′10.69″N, 14°57′13.56″E) and Owczary (52°28′53.59″N, 14°37′30.43″E). The assemblages containing the fern were identified as representing the association Ceratophyllo-Azolletum filiculoidis Nedelcu 1967. Azolla has also entered the phytocoenoses of other floating plant communities. Mean values of several chemical parameters of the water like conductivity, salinity, total dissolved solids and nitrate content at the fern stations were significantly higher than the mean values found in the Azolla-devoid sites while the values for hardness (Ca), pH and visibility (Secchi Disc depth) were lower at Azolla sites. In 2017, this alien fern was found to had successfully overwintered in the main bed of Oder river, and its further spread down the river was observed. The appearance of Azolla filiculoides in the Oder may be associated with an exceptionally warm winter; its emergence may also accelerate the species' invasion in Poland.
Dolichols isolated from leaves of the fern Matteucia struthiopteris were present as a mixture of prenologues composed of 14 up to 20 isoprene units with Dol-16 dominating. They comprised approximately 0.004% of the fresh weight of fresh plant tissue and were accompanied by traces of polyprenols (Pren-14 up to Pren-17, Pren-16 dominating). Their structure was confirmed by electropray ionization mass spectrometry (ESI-MS). This is the first time that dolichols have been reported as dominating polyisoprenoid alcohols in plant photosynthetic tissue.
The paper reports the results of karyological studies on seven fern species from the Polish Carpathians. Their chromosome numbers counted in root tip mitoses are reported for the first time from Poland. The specimens originated from the Tatra Mts and Pieniny range. Chromosome numbers were established for Asplenium viride (2n = 72), Athyrium filix-femina (2n = 80), Dryopteris filix-mas (2n = 164), Gymnocarpium dryopteris (2n = 160), Gymnocarpium robertianum (2n = 160), Phegopteris connectilis (2n = 90) and Phyllitis scolopendrium subsp. scolopendrium (2n = 72). The chromosome numbers from the Polish material match those reported from other countries. Information on the distribution and habitats of the investigated fern species is also provided.
Several fern remains are described from the para-autochthonous early late Ladinian flora of the Monte Agnello (Dolomites, N-Italy). The plants are preserved in subaerially deposited pyroclastic layers. Some ferns, known already from the Anisian and Ladinian of this area, are confirmed (Neuropteridium elegans), but several taxa are described for the first time (Phlebopteris fiemmensis sp. nov., Cladophlebis ladinica sp. nov., Chiropteris monteagnellii sp. nov.). Cladophlebis sp. and some indeterminable fern remains cannot yet be assigned to any family. Phlebopteris fiemmensis is now the oldest formally established species in the genus. The fern family Dipteridaceae (Thaumatopteris sp. and some fragments probably belonging to the Dipteridaceae because of their venation) has not been recorded previously from European sediments as old as the Ladinian. Although stratigraphically attributed to the late Ladinian, the flora is markedly distinct from other Ladinian floras of the Dolomites and the Germanic Basin. The flora from Monte Agnello shows a higher diversity in ferns than coeval floras from this area although characteristic elements of the Ladinian of the Dolomites such as Anomopteris and Gordonopteris are missing. The new flora misses also the Marattiales (e.g., Danaeopsis, Asterotheca) and other elements such as Sphenopteris schoenleiniana, typical for the Ladinian of the Germanic Basin.
Extinct seed ferns (Pteridospermophyta) fill a key position in the evolutionary tree of seed plants. Their reproductive structures enable to recognition of the interrelationship within seed ferns and other seed plants descending from them. Seed fern reproductive structures from Poland are very rarely found and very poorly known, especially the pollen organs to which this paper is dedicated. A new genus and species Silesiatheca formosa Pacyna & Zdebska, gen. et sp. nov. of lyginopteridalean aggregated synangia was recognized based on specimens preserved within sideritic nodules from Sosnowiec. The genus Codonotheca is recognized from European Carboniferous for the first time. The least evolutionarily advanced species of Codonotheca − Codonotheca silesiaca Pacyna & Zdebska sp. nov., is described based on specimens with weakly expressed radial symmetry and slight fusion of the sporangia. This species matches the hitherto hypothetical simplest species of Codonotheca very well, constituting the initial form for all the more compound pollen organs of Medullosales. For the first time in the European Carboniferous the North American species Aulacotheca campbellii (White, 1900) Halle, 1933 is recognized. A new emended diagnosis for the typical species Boulayatheca fertilis (Kidston, 1914) Taylor & Kurmann, 1985 is proposed, based on specimens from Poland. Two new species of genus Boulayatheca Taylor & Kurmann, 1985 are recognized. Boulayatheca ciliata Pacyna & Zdebska, sp. nov. with a synangium surface covered with hairs (a feature hitherto unknown in the Boulayatheca genus) and Boulayatheca saganii Pacyna & Zdebska, sp. nov. with a markedly elongated synangium. Occurrence of Whittleseya Newberry, 1853a emend. Halle, 1933 typical species − Whittleseya elegans Newberry, 1853a was confirmed in Lower Silesia based on new specimens. Some specimens referred to earlier in the literature as W. elegans were ascribed here to the new species W. silesiaca Pacyna & Zdebska, sp. nov., which differs from typical species in synangium shape and sporangia clustering into groups. The new species Whittleseya campanulata Pacyna & Zdebska, sp. nov. has a large synangium and sporangia clustering into groups. Heterogeneity within the genus Whittleseya is recognized, where some species, including those new described here, probably formed compound synangia. The aggregated synangia of Medullosales are recognized in thecompression state of preservation for the first time they are the base for establishing a new genus and species Kotasotheca annaeadamii Pacyna & Zdebska, gen. et sp. nov. The genus Dolerotheca Halle, 1933 was recognized in the Polish Carboniferous for the first time and a new species Dolerotheca migierii Pacyna & Zdebska, sp. nov. was described. T.N. Taylor’s model of evolution of medullosalean pollen organs was confirmed, and the new taxa described were included within it.
In this paper the content of Mn, Fe, Zn, Cu, Pb, Ni, Cr and Cd was studied in forest floor plants of Puszcza Biaµa Forest. Test plants used were lichens, mosses, club-mosses, ferns, convallaria, and bilberries. Puszcza Biaµa Forest belongs to a region free from pollution by heavy metals. Higher content of manganese, as compared with iron, was found in the following plants: Cladonia clavatum (wolf's claw), Dryopteris filix-mas (shield fern), Convalaria maialis (convallaria), and Vaccinium myrtillus (bilberry). In lichens and mosses the proportion was the opposite: they contained more iron than manganese. The stated concentration of Zn, Cu, Pb, Ni, Cr and Cd was very little differentiated considering particular plant species of forest floor. And those were appreciated at the natural level, typical for the unpolluted area of Poland.
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