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The effect of feeding ration, enriched in synthetic and natural ß-carotene on the selectedindicators of health condition of the cows and on reproduction parameters. The studies were conducted on 40 Simmental cows, classified into 4 groups, 10 animals in each group. The experiment was performed since the 4th week before calving until 12 week of lactation. In group I (control), standard nutrition, based on grass silage and maize silage was employed. In group II 400 mg of synthetic ß-carotene/cow/day to the ration was introduced. In group III, a part of maize silage was replaced by pumpkin silage in such ratio as to receive by 400 mg higher intake of natural ß-carotene/cow/day as compared to group I. The rations in group IV were not balanced in respect of the content of ß-carotene; there was only the replacement of 60% DM of maize silage by the pumpkin silage in this group. During the studies, blood samples were collected: to 5 hours after calving and on 4 and 12 week of lactation. Milk samples were collected on 4, 8 and 12 week of lactation and on 23 day after the first insemination. Morphological parameters of blood and concentration of ß-hydroxy-butyric acid in blood serum were analysed. In milk, somatic cell count and the level of progesterone on 23 day after insemination were determined. In group I during the following measurements were found the lowest levels of part of the morphological parameters of blood: after calving the lowest level of RBC (significant difference compared to group II), at 4th week of lactation the lowest level of HCT, and at 12 week of lactation RBC and HCT. The significant differences in somatic cell count in the milk, were recorded in the 8th week of lactation –significantly higher in group I as compared to group III. The significant differences between the groups in respect of BHM acid concentration was found only after calving. The highest BHM level occurred in the cows from group I and it was significantly higher in relation to group II and IV. In the cows from groups II, III and IV, the first heat occurred earlier; these animals had also shorter inter-gestation period. Also, the progesterone level, as determined on 23 day after insemination, was higher in the milk of cows from groups II, III and IV as compared to the animals from group I.
Two trials were conducted. In trial I three lactating Polish Holstein-Friesian (PF) cows fitted with rumen cannulae were used in a 3 x 3 Latin square design. In each feeding cycle animals were offered one of three following diets: (1) without supplementation (C diet – control), (2) C diet supplemented with fish and rapeseed oils blend 1:1 (FR diet), and (3) C diet supplemented with commercial protected fat (CPF diet). In trial II, ten lactating PF cows were offered C diet, and another ten – FR diet. In both trials fat supplements constituted up to 4% of the diet dry matter. FR diet strongly affected the fatty acid composition of rumen fluid and milk of cows. Content of oleic acid and Rother MUFAs increased, while that of total SFAs dropped in the rumen and, in both trials, in milk. The FR diet led to increase in the c9 t11 CLA (conjugated linoleic acid) isomer content of milk in both trials.In trial II the t10 c12 CLA concentration of milk increased in cows fed the FR diet. It is evident that during the summer feeding based on fresh green forage, good results regarding milk fatty acid content can still be improved by feeding rapeseed oil and fish oil.
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