Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 51

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  feces
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
The purpose of the studies was to determine, by means of bacteriological and molecular methods, the occurrence of Y. enterocolitica in the faeces of dogs, as well as to assess the degree of their spread in the urban environment. The faeces were collected from the lawns of six large districts of Olsztyn. In order to isolate Y. enterocolitica, "warm culture" (ITC/CIN) and "cold culture" (PSB/CIN) were used in parallel, together with biochemical tests. Next, genomic DNA of Y. enterocolitica was isolated and αil, ystA, and ystB genes were detected with the use of the multiplex PCR method. A relatively frequent occurrence of Y. enterocolitica strains in canine faeces contaminating the urban lawns of Olsztyn was demonstrated. The greatest percentage of contaminated faecal samples was found in the area of large housing estates. By means of molecular tests, the presence of ystB gene only, in the absence of αil and ystA genes, was demonstrated in the Y enterocolitica genome, which could indicate a potentially pathogenic biotype 1A. Therefore, it seems important to keep monitoring the changes, which occur within this species of microorganisms, the epidemiological situation of yersiniosis in human and animal populations, as well as to continue the studies on the epidemiology of Y enterocolitica infections also in the context of a reservoir of animals accompanying a man.
The domination of microorganisms characterized by excessive activity of the so-called fecal enzymes may be one of the reasons of the large intestine cancers. These enzymes are mainly those that belong to the hydrolase and reductase classes and their excessive activity may lead to disorders in the functioning of the digestive tract. The aim of tise research was to determine the activity of β-glucuronidase and β-glucosidase of Lactobacillus and Enterococcus strains isolated from the feces of healthy children, aged 1 and 8, and adults, aged 30 and.80. The analysis included 10 strains isolated from the feces of individuals in each of the age groups. β-glucuronidase activity in the case of the isolates from children, depending on the strain, equaled from about 0.15 mM/h/mg of protein to 0.26 mM/h/mg of protein and was lower, respectively, by 52.35% and 57.81%, than the β-glucosidase activity. Simultaneously, the activity of the Lactobacillus enzymes from children was 2.4 times higher, and in case of the isolates obtained from adults they were 4.6 and 2.7 times higher than the activity of the Enterococcus enzymes. The highest β-glucuronidase activity was observed in Lactobacillus isolates coming from an 80-year-old subject. The differences between the activity of Enterococcus β-glucuronidase isolated from the feces of 1 and 8 year old children were statistically insignificant. On the other hand, in the case of the subjects aged 30 and 8 the isolates were characterized by activity lower by, respectively. 48% and 37% than the isolates coming from children. The highest β-glucosidase activity was discovered in the case of Lactobacillus and Enterococcus coming from children, which was higher by 32% than the activity of the isolates from adult persons. Therefore, it was determined that the activity of β-glucuronidase of Lactobacillus strains isolated from feces from people aged 80 was the highest, and the isolates of the examined microorganisms coming from children were characterized by the highest β-glucosidase activity.
The diagnosis of brucellosis is mainly based on serological tests. All animals classified as serologically positive are obligatorily slaughtered and subjected to bacteriological examination. B. abortus has not been reported in Poland since the eradication of bovine brucellosis in 1980. On the other hand, B. suis biovar 2 is sporadically isolated from cattle. In accordance with the instructions of the Chief Veterinary Officer, samples of feces from animals slaughtered following positive serological results for brucellosis have been examined for the presence of Yersinia enterocolitica O:9 since 2011. Because of the similarity of its O-polysaccharide component of S-LPS with that of Brucella, this microorganism is considered to be a major contributory factor of false positive serological reactions (FPSR). The paper presents the results of the bacteriological and molecular examination of feces from 26 cows seropositive for brucellosis and 30 healthy cows, negative for brucellosis, for the presence of Y. enterocolitica O:9. Y. enterocolitica O:9 was found in 7 of the positive cows, whereas all samples from cows negative for brucellosis were free from this bacteria. These results indicate that Y. enterocolitica O:9 may be responsible for some of the positive results for brucellosis in cattle.
Examination of 98 gold skink, Eumeces schneiderii Daudin, 1802 from Alexandria and Marsa-matroh Governorates, Egypt revealed oocysts of Choleoeimeria baltrocki (Daszak et Ball, 1991) n. comb. in the gall bladder and faeces. The prevalence of the infection was 40.8%. Sporulated oocysts were found in the faeces and the gall bladder contents. Sporulated oocysts were tetrasporocystic, cylindrical, reaching a mean of 38.7 (36–42) × 19.9 (17–25) μm with bilayered smooth and colourless wall. The dizoic sporocysts were subspherical and measuring 10.8 (9.5–13) × 9.3 (8–10.5) μm with a large globular sporocyst residuum. Sporocyst wall was bilayered joined by meridional suture. Sporozoites were crescent-shaped, blunt at one end and slightly tapered at the other. Endogenous stages (meronts, gamonts, gametes and young oocysts) were confined to the gall bladder epithelium and the infected cells were hypertrophied. Based on the morphological features of the exogenous stages and endogenous development of the present parasite, its generic affiliation was revised and placed into the genus Choleoeimeria.
Big brown bats (Eptesicus fuscus) were genotyped from their feces using three sets of microsatellite primers. Genotypes obtained from bat fecal DNA consistently matched the genotypes obtained from DNA extracted from wing membrane tissue of the same bat. Identical microsatellite genotypes were also obtained from multiple fecal DNA samples from the same bat. DNA obtained from feces using a modification to the DNeasy tissue kit (Qiagen) amplified from 92% (83/90) of the samples upon the first PCR. The use of fecal DNA provides opportunities for addressing ecological and behavioral questions for animals that are difficult to capture, rare, or endangered.
Falcaustra sinensis sp. nov. collected from the feces of Indotestudo elongata (Blyth) is described and illustrated. The new species can be distinguished from the congeners by the arrangement of caudal papillae of the male (6 pairs precloacal, 4 pairs postcloacal, 1 median), length of spicules (3.84–4.74 mm) and the presence of pseudosucker.
The interaction of lead and calcium assimilation was examined in the soft tissues, shell and faeces of the soft tissues, shell and faeces of the garden snail an induced calcium stress, shell wounding. This is known to mobilise Ca both from the shell and soft tissues, conditions which are likely to affect Pb assimilation by each component Following wounding, snails were fed an artificial diet for 7 days with some combination of Pb and/or Ca and Mg in a 22 factorial experiment Dietary Pb raised the concentration of this metal in all tissue components, though the scale of the increase was the same in both wounded and unwoun- ded snails. The smallest increase was in the shell. Ca and Mg levels in the hepatopancreas remain unchanged by the dietary levels of any treatment, but were raised in the rest of the soft tissues. Significantly, the high Pb diet is almost equally effective as wounding in lowering the Ca in this component. Injuring the shell had little effect on the uptake of Pb by the snails.
We examined the characteristics of fluorescent powder as dye in bait for the purpose of studying individual foraging areas of the root vole Microtus oeconomus (Pallas, 1776). Colours were visible in the faeces 2-3 h after consumption, and were still evident 36-48 h after the removal of bait. It was possible to distinguish up to four different colours in faeces from one individual if the appropriate combination of coloured powder was used. The method is a better alternative to other markers commonly used in bait because only a small extra sampling effort is needed during trapping, and the observation of colours is relatively easy in UV-light. Because the persistance of the colour powder in the animal is relatively short, the method facilitates studies of short-term changes in foraging areas.
In our studies two primer pairs within the VP2 protein gene of canine parvovirus were chosen on the base of the sequence of Polish isolates. The sensitivity of detection of canine parvovirus in stool samples by Nested PCR was increased 60% in comparison with the standard PCR method and it was 30% higher than the virus isolation in tissue culture. A simple procedure of sample preparation with the use of Chelating resin was used and it made the Nested PCR easily applicable in rapid confirmation of the diagnosis of parvovirus infection.
In the study of the concentration of thiamine in the plasma, liver, urine and faeces as indices of its utilisation we found that cellulose enhances the bioavailability of this vitamin in vivo; however, pectin has an opposite effect, that is, it lowers its bioavailability.
To test the feasibility of using field-collected scats as a source of DNA in the study of the least weasel Mustela nivalis nivalis Linnaeus, 1766, DNA was extracted from scat samples collected from captive weasels using a modified extraction protocol. Using universal primers, the control region of the mitochondrial genome was successfully amplified from scat-extracted DNA. This amplification resulted in two products; one equivalent in size and sequence to the product obtained from tissue-extracted weasel DNA, and the other slightly larger and equivalent in size and sequence to the domestic house mouse Mus musculus, the food source of the captive weasels. This demonstrates the reliability of DNA extraction from scats, as well as the possibility, under favourable circumstances, of identifying the prey species from the same samples. In addition, we attempted to amplify microsatellite loci from both tissue and scat-extracted DNA using six primer pairs designed for other mustelids, the American mink Mustela vison and the wolverine Gulo gulo. While three loci, Mvi57 (American mink), Ggu216 and Ggu234 (wolverine), were found to be polymorphic in the least weasel, amplification of these loci from the scat extracted DNA was only successful for approximately half of the samples. Although further work is needed, the present results suggest that it is possible to use scats as a source of DNA in field studies of the least weasel.
The food-handling and reingestive behaviour of feces by Ctenomys pearsoni Lessa and Langguth, 1983, was studied in the laboratory. All animals studied handle grasses with dexterity with one or both forepaws, and vigorously shaked it up and down before ingestion. Reingestion of feces occur frequently during resting periods and between feeding bouts. While performing both behaviours, C. pearsoni adopt a posture that not increase its height, what could be considered as and adaptation to the burrow space. Some convergences and divergences in the patterns of food-handling and reingestion of feces between Ctenomys and other subterranean rodents genera were remarked. We propose that the food-handling pattern is related to the cleaning of food; while the reingestion pattern might be related to water economy and to recover some particular nutrients.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.