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Good DNA from bat droppings

100%

Puechmaille S. J., Mathy G., Petit E. J.

Acta Chiropterologica

2007

tom 09

nr 1

Amplification of a mitochondrial DNA fragment was used to compare the efficiency of five methods for extracting DNA from bat droppings. The Qiagen DNA Stool Kit, which yielded > 90% mtDNA amplification success, was chosen to extract DNA from 586 samples taken over two years in three French colonies of the lesser horseshoe bat (Rhinolophus hipposideros). Samples, for which mtDNA amplification was successful, were subject to the multiplex amplification of eight microsatellite loci. This resulted in > 95% amplification success over 12,592 PCRs. Allelic dropout (ADO) and false allele (FA) rates were low, and consequently, sample and locus quality indexes (QI) were high. These results demonstrate that large scale noninvasive studies of bat colonies are possible.

Microsatellite genotypes of big brown bats (Eptesicus fuscus: Vespertilionidae, Chiroptera) obtained from their feces

100%

Vege S., McCracken G. F.

Acta Chiropterologica

2001

tom 03

nr 2

Big brown bats (Eptesicus fuscus) were genotyped from their feces using three sets of microsatellite primers. Genotypes obtained from bat fecal DNA consistently matched the genotypes obtained from DNA extracted from wing membrane tissue of the same bat. Identical microsatellite genotypes were also obtained from multiple fecal DNA samples from the same bat. DNA obtained from feces using a modification to the DNeasy tissue kit (Qiagen) amplified from 92% (83/90) of the samples upon the first PCR. The use of fecal DNA provides opportunities for addressing ecological and behavioral questions for animals that are difficult to capture, rare, or endangered.

Ograniczanie wyników

2 Acta Chiropterologica

1 Mathy G.

1 McCracken G. F.

1 Petit E. J.

1 Puechmaille S. J.

1 Vege S.

1 2007

1 2001

Wyniki wyszukiwania

Good DNA from bat droppings

Microsatellite genotypes of big brown bats (Eptesicus fuscus: Vespertilionidae, Chiroptera) obtained from their feces