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1

The effect of explant type on somatic embryogenesis induction in Pisum sativum L.

100%
Gorska-Koplinska K., Zrobek-Sokolnik A., Gorecki R. J., Michalczyk D. J.
Polish Journal of Natural Sciences
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2010
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tom 25
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nr 3
The effect of explant type on somatic embryogenesis induction in Pisum sativum (cv. Oskar and an unregistered line HM-6) was studied. Shoot apices, leaf primordia, and epicotyl fragments of axenically grown, etiolated seedlings, as well as embryonic axes and cotyledon fragments isolated from zygotic embryos at different stages of development, were used as explants. Somatic embryogenesis was induced essentially as described by Griga in 1998 – MS salts and sucrose, B5 Gamborg vitamins, picloram (2.5 μM). After induction period (14 days) all cultures were transferred to the differentiation medium (basal medium as above, auxin omitted). Both in Oskar and HM-6, only shoot apices developed somatic embryos and (with significantly lower frequency) adventitious shoots.
2

Effect of cytokinin concentration and explant type on micropropagation of Impatiens x walleriana Hook

100%
Witomska M., Lukaszewska A.
Annals of Warsaw Agricultural University. Horticulture (Landscape Architecture)
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2003
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tom 24
3
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Effect of plant growth regulators and explant types on induction and growth of callus of Primula veris L.

84%
Morozowska M., Wesolowska M., Glowicka-Woloszyn R., Kosinska A.
Herba Polonica
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2013
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tom 59
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nr 3
Primula veris L. (Primulaceae) is a well-known medicinal herb. The callus induction response of three explant types: roots, cotyledons, and hypocotyls of four-week-old cowslip seedlings were evaluated. The highest statistically different callus induction rate was 93.6% and was obtained from root explants on MS medium supplemented with 0.1 mg/l BA and 5.0 mg/l PIC. Calli also appeared on 83.3% of cotyledon explants on MS medium supplemented with 1.0 mg/l BA and 3.5 mg/l 2,4-D and on 81.0% of root explants on MS medium supplemented with 0.1 mg/l KIN and 2.0 mg/l 2,4-D. These values were not statistically different. The average time required for callus initiation was 4 to 6 weeks, however, it depended on the explants type. The most suitable condition for callus proliferation and growth was MS medium with 0.5 mg/l TDZ and 0.1 mg/l NAA, and with 1.0 mg/l BA and 2.0 mg/l or 3.5 mg/l 2,4-D. No light conditions proved to be more favourable for cowslip calli induction and growth.
4
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The effect of 6-benzylaminopurine, thidiazuron and the type of explants on in vitro propagation of Yucca elephantipes Regel

84%
Kozak D.
Acta Scientiarum Polonorum. Hortorum Cultus
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2010
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tom 09
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nr 3
Yucca elephantipes is an important commercial ornamental pot plant, excellent for growing in flats, patios or winter gardens. Traditional vegetative propagation of the most decorative yuccas is complicated due to a very low rate of propagation, so in vitro culture is an alternative method for commercial propagation of these plants. The influence of BA (0.4, 2.2, 4.4, 11.1, 22.2 µM) and TDZ (0.5, 2.3, 4.5, 11.4, 22.7 µM) on shoot multiplication of Yucca elephantipes Regel on Murashige and Skoog (MS) medium was studied. Explants cultured on medium without growth substances were used as a control. The two types of explants used in the experiment: shoot tips and nodal segments of shoots, were obtained from aseptically grown shoot clusters. When comparing regeneration capability of 2 types of Yucca elephantipes explants, it was found that more newly formed shoots and roots were obtained from nodes. The highest formation of shoots was obtained from nodes on MS medium supplemented with 4.5 µM TDZ or 11.1 and 22.2 µM BA (6.5, 6.0, 5.8, respectively). The shoots regenerated from nodes showed best elongation. On the control medium and on the media with the lowest level of BA or TDZ, their average length was 31.0–37.8 mm. The growth regulator-free medium and the media with a low level of BA were the most effective in inducing roots.
5
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The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro

59%
Kozak D., Parzymies M., Swistowska A., Marcinek B., Ismael B. S.
Acta Scientiarum Polonorum. Hortorum Cultus
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2019
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tom 18
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nr 4
Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
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