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Anabolic-androgenic steroids (AAS) are used in high doses by athletes to improve athletic ability, physical appearance, and muscle mass. Unfortunately, the abuse of these agents has significantly increased. It has been established that exercise and high doses of AAS may influence the hypothalamic-pituitary gonadal (H-P-G) axis, which can in turn affect the ultrastructure of the testes. However, the effect of the combination of exercise and high doses of AAS on the ultrastructure of the testes is not known. This study was undertaken in order to examine the combination effects of swimming exercise and supraphysiological doses of nandrolone decanoate on the ultrastructural changes in rat testes. Five groups of male Wistar strain albino rats were treated as follows for 8 weeks: solvent of nandrolone decanoate (peanut oil) as a vehicle (sham); nandrolone decanoate (ND) (10 mg/kg/week) — ND; exercise (1 h/day, 5 days a week) — exercise; ND (10 mg/kg/week) and exercise (1 h/day, 5 days a week) — ND-EX; and sedentary control without any injection or exercise — control. Ultrastructural changes in the rat testes were characterised by transmission electron microscopy. The number and size of Leydig cells were considerably decreased in the interstitial space in the experimental rats. The increased thickness and irregular wavy multilaminar appearance of basement membrane in the treated animals, especially in the ND-EX group, are associated with well developed myoid cells. Cytoplasm vacuolisation, vesicular-like crista of the mitochondria, numerous lipid droplets, and lysosome and phagolysosome in Sertoli cells were significantly observed in the experimental groups. Several apoptotic germ cells were considerably observed in the experimental rats (p ≤ 0.05). Exercise training seems to increase the extent of ultrastructural changes caused by supraphysiological doses of ND in rats, which in turn may affect fertility. (Folia Morphol 2010; 69, 3: 138–146)
Introduction. The main part of skeletal muscle adenosine- 5'-triphosphate (ATP) is restored by inosine monophosphate (IMP) reamination in the purine nucleotide cycle. The intramuscular resources of IMP may be resynthesized via the quick and economical salvage pathway, in which muscle hypoxanthine (Hx) is reconverted to IMP by hypoxanthineguanine phosphoribosyl transferase (HGPRT). IMP is subsequently reutilized in the adenine nucleotide (AdN) pool. Inosine and Hx, which flow out of the skeletal muscle, represent the loss of AdN precursors. In the latter case, full restoration of resting ATP levels depends on a slow and energy-consuming de novo pathway. Plasma Hx and erythrocyte HGPRT are indirect indicators of muscle metabolism, particularly of AdN degradation, that reflect exercise- and training-induced muscle energy status. Results. Our analyses of long-term training cycles in different sports show that plasma Hx concentration and erythrocyte HGPRT activity significantly change in consecutive training phases. Both high-intensity sprint training and endurance training incorporating high-intensity exercise lead to a decrease in plasma Hx levels and an increase in erythrocyte HGPRT activity. The lowest Hx concentration and the highest HGPRT activity are observed in the competition phase characterised by low-volume and high-intensity training loads. Training cessation in the transition phase brings about a reverse phenomenon: an increase in Hx levels and a decrease in HGPRT activity. Conclusions. Low plasma purine levels indicate that the administered training adapts the athletes to high-intensity exercise (more economical AdN use, limited purine efflux from muscle into the blood). Such an adaptation is of great importance for contemporary elite athletes. Purine metabolites are more sensitive markers of training status and better performance predictors than typical biochemical and physiological indicators (e.g. blood lactate and oxygen uptake) in highly-trained athletes of different specializations and ages. The use of Hx and HGPRT for monitoring and control of the training process is worthy of consideration.
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