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Four ethanologenic ethanol-tolerant yeast strains, Saccharomyces cerevisiae (ATKU132), Saccharomycodes ludwigii (ATKU47), and Issa- tchenkia orientalis (ATKU5-60 and ATKU5-70), were isolated by an enrichment technique in yeast extract peptone dextrose (YPD) medium supplemented with 10% (v/v) ethanol at 30°C. Among non-Saccharomyces yeasts, Sd. ludwigii ATKU47 exhibited the highest ethanoltolerance and ethanol production, which was similar to S. cerevisiae ATKU132. The maximum range of ethanol concentrations produced at 37°C by S. cerevisiae ATKU132 and Sd. ludwigii ATKU47 from an initial D-glucose concentration of 20% (w/v) and 28% (w/v) sugarcane molasses were 9.46–9.82% (w/v) and 8.07–8.32% (w/v), respectively.
Background. The difficult position of Polish agriculture, including one of its branches, i.e. sugar industry, is conducive of search for solutions aiming at an improvement of the condition of industry. One of the potential solutions in this respect may be to focus on alternative raw materials and search for ways to overcome recession in renewable energy sources. The aim of this work was to evaluate the possibilities of using non-starchy materials - sugar materials, without enzymatic treatment for ethanol production using selected yeast strains. Material and methods. Sugar beet pulp and thick juice, as a semi product from sugar beet, were fermented. The efficiency of the process was assessed using two Saccharomyces cerevisiae preparations - Ethanol Red, Fermiol. Fermentation was run for 72 h at 30°C. Quality of produced raw distillates was evaluated using the GC method. Results. The research on fermentation processes showed that sugar beet pulp let obtain higher ethanol yield - 87% of theoretical than sugar beet thick juice - 84% of theoretical, both for Ethanol Red and Fermiol yeast preparations. Moreover, it was exhibited that the increase of sugar concentration in the fermentation medium obtained from thick juice, statistically importantly influenced ethanol yield decrease, for both yeast preparations. The distillates’ quality analysis showed the influence of raw materials and microorganism used for fermentation on pollution degree. Distillate obtained from thick juice was characterised with the lowest by-products content after fermentation with Ethanol Red. Conclusions. The results make additional possibilities for sugar beet utilization in distillery industry and new markets using production surpluses both for sugar beet and its semi- -product - thick juice.
Coculture of xylose-fermenting yeast P. stipitis CCY 39501 and respiratory deficient mutant of S. cerevisiae V30 designated as V30 I 40 was used for ethanol fermentation on a medium containing glucose and xylose mixture and compared to P. stipitis monoculture or coculture of P. stipitis and S. cerevisiae V30. Batch fermentations were carried out on a model medium or on a medium containing both sugars derived from direct saccharification of either wheat straw or birch sawdust. The yields obtained were 0.38 g/g, 0.34 g/g and 0.4 g/g for model medium, wheat straw and birch sawdust hydrolysates respectively, after cofermentation of P. stipitis with RD mutant V30 I 40. The results confirmed the application of this coculture for ethanol fermentation of sugars derived from lignocellulosic hydrolysates.
A total of 24 yeast strains were tested for their capacity to produce ethanol, and of these, 8 were characterized by the best ethanol yields (73.1181.78%). The most active mutant Saccharomyces cerevisiae ER-A, resistant to ethanol stress, was characterized by high resistance to acidic (pH 1.0 and 2.0), oxidative (1 and 2% of H₂O₂), and high temperature (45 and 52°C) stresses. During cultivation under all stress conditions, the mutants showed a considerably increased viability ranging widely from about 1.04 to 3.94-fold in comparison with the parent strain S. cerevisiae ER. At an initial sucrose concentration of 150 g/l in basal medium A containing yeast extract and mineral salts, at 30°C and within 72 h, the most active strain, S. cerevisiae ER-A, reached an ethanol concentration of 80 g/l, ethanol productivity of 1.1 g/l/h, and an ethanol yield (% of theoretical) of 99.13. Those values were significantly higher in comparison with parent strain (ethanol concentration 71 g/l and productivity of 0,99 g/l/h). The present study seems to confirm the high effectiveness of selection of ethanol-resistant yeast strains by adaptation to high ethanol concentrations, for increased ethanol production.
Background. Bacterial contamination is a major problem for commercial fuel ethanol production in distilleries all over the world. The contaminating microorganisms produce acetic and lactic acid that has a detrimental effect on fermentation efficiency. The aim of this work was to calculate the number of bacterial contaminants in some distilleries. Moreover, in this study it was signified what kind of bacteria contaminate ethanol production process. Material and methods. Grains were obtained from five distilleries from some regions in Poland, in this work hereafter referred to as α, β, γ, δ, and ε distilleries. Corn was the raw material in the α, β, and γ distilleries, triticale in δ distillery, and rye in the ε one. From these five distilleries, sweet mashes during fermentation and after it, were also analysed. The total number of microorganisms, the number of lactic acid bacteria, the number of anaerobic bacteria and the quantity of yeasts and moulds in raw materials were calculated. Results. The number of total viable bacteria (CFU/g), lactic acid bacteria (CFU/g), anaerobic bacteria (CFU/g), moulds, and yeasts (CFU/g) occur in the samples were determined. In all distilleries tested, all groups of microorganism were present. Conclusions. The results of our study show that all tested distilleries have a lot of difficulties with microbiology pollution which leads to a decrease of ethanol production and economical problems. From the economical point of view, reduction of microbial contamination makes it possible to increase the production volume
Materiał badawczy stanowił odpadowy granulat ziemniaczany pochodzący z przemysłu. Zaciery o 20% stężeniu surowca przygotowano w warunkach laboratoryjnych. Proces fermentacji prowadzono w temperaturze 30°C z udziałem drożdży Saccharomyces cerevisiae Etanol Red. W wyniku fermentacji zacierów uzyskano 67,5 g etanolu na litr zacieru. Proces przebiegał z wydajnością praktyczną na poziomie powyżej 87,8%. Podczas fermentacji drożdże charakteryzowały się dobrym stanem fizjologicznym.
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