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Contamination with microscopic fungi measured by the concentration of ergosterol in dusts of various types of wood with different granulation

100%

Szablewski T., Szwajkowska-Michałek L., Pedzik M., Rogozinski T., Stupor-Szablewska K.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2021

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tom 113

2

Fungi biomass in lowland rivers of North-Eastern Poland: effects of habitat conditions and nutrient concentration

100%

Gorniak A., Wiecko A., Cudowski A.

Polish Journal of Ecology

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2013

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tom 61

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nr 4

Fungi occur in almost all types of waters in the world and play an important role in many processes in the environment, particularly in organic matter decomposition. During the last several decades, extensive knowledge was obtained on the ecology of aquatic fungi, but on fungal biomass in the water column less. The study was aimed at simultaneous determination of seasonal and spatial variability of aquatic fungi biomass in the river waters in relation to selected physicochemical water parameters and trophic state in the 27 lowland rivers of north-eastern Poland with length between 17 and 308 km and mean annual discharge 1 – 100 m³ s⁻¹. The chemical and mycological evaluation was provided on the basis of 64 water samples taken in the July and October 2010. The chromatographic method of determination of seston ergosterol as a specific organic compound typical for the majority of fungi, was applied. Fungi biomass was higher in summer than in autumn. The particulate organic carbon (POC) and dissolved organic carbon (DOC) concentrations had no effect on fungal biomass in the studied river, as well as forest cover of the catchment and river discharge. Statistically significant correlations of fungal biomass in the river water with concentrations of ammonium (N–NH₄⁺), Kjeldahl (NKjel), total organic (TON) and total nitrogen (TN) and dissolved (DP) and dissolved organic phosphorus (DOP) were found. The principal component analysis indicates relations between (TIN) and phosphorus (SRP) and particulate phosphorus (PP) and freshwater fungi, specially in summer when aquatic fungi are able to modify the structure of the nutrients contained in the water column.

3

Comparison of various methods for determination of antifungal activity of Bacillus coagulans

100%

Czaczyk K., Trojanowska K., Stachowiak B., Zabielski J.

Journal of Plant Protection Research

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2002

|

tom 42

|

nr 4

4

Ergosterol biosynthesis inhibition: a target for antifungal agents

100%

Barrett-Bee K., Dixon G.

Acta Biochimica Polonica

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1995

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tom 42

|

nr 4

The isoprenoid sterols play a crucial role in the viability of all fungi; those unable to synthesise ergosterol because of inhibition, growth conditions or mutation must take it up from the environment. A range of compound types have been discovered which interfere with the biosynthetic pathway from acetate to ergosterol and these compounds have antifungal actions. Inhibition of several of the steps has yielded agents which have been used with great success as medical and agrochemical agents. The most important biosynthetic steps that have been exploited are inhibition of squalene epoxidase, (the allylamines and tolnaftate) C14 demethylation (the azoles), A7,8 isomerase and A14 reductase which are inhibited by the morpholines. Recent research has shown that inhibition of C24 methyltransferase and C4 demethylation also yield antifungal agents. Combination studies demonstrate that synergy between agents of different types can be measured. Fungicidal effects were observed when a combination of two fungistatic agents was used.

5

Ergosterol jako wskaznik mikroflory grzybowej ze szczegolnym uwzglednieniem jego zawartosci w zbozach oraz produktach zbozowych

84%

Perkowski J., Busko M.

Przegląd Zbożowo-Młynarski

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2006

|

tom 50

|

nr 08

10-13

6

Zawartosc ergosterolu w zbozowych produktach spozywczych

84%

Stuper K., Perkowski J.

Żywność Nauka Technologia Jakość

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2008

|

tom 15

|

nr 5

71-77

Zawartość biomasy grzybowej wpływa w istotny sposób na ocenę jakości produktów spożywczych. W pracy przedstawiono zawartość biomarkera biomasy grzybowej (ergosterolu-ERG) w ziarnie zbóż, produktach śniadaniowych zawierających otręby oraz płatki zakupione w sieci sklepów detalicznych na terenie miasta Poznania w roku 2008, jak również w mąkach, a także w produktach po przemiale laboratoryjnym. Największą średnią zawartość ERG stwierdzono w otrębach owsianych - wynosiła ona 30,71 mg/kg, natomiast najmniejszą spośród analizowanych otrąb oznaczono w otrębach pszennych - 9,98 mg/kg. Spośród płatków najwięcej ERG stwierdzono w płatkach owsianych - 8,04 mg/kg, a najmniej w pszennych - 0,97 mg/kg. Produkty zawierające dodatki w postaci suszu owocowego zawierały większą ilość ERG niż analogiczne produkty bez dodatków. Przebadano również mąki żytnie, jęczmienne, owsiane oraz pszenne. Porównano zawartość ERG w mąkach w stosunku do otrąb stwierdzając, że zawartość tego metabolitu jest porównywalna we wszystkich zbożach i jest mniejsza w mące średnio o około 97 %. Zawartość tego metabolitu w otrębach była większa w stosunku do ziarna niepoddanego obróbce i wynosiła około 50 %. Obok ww. produktów przemiału badano również zawartość ERG w ziarnie pobranym także w 2008 r. z silosów zbożowych. Najwięcej ERG stwierdzono w ziarnie owsa, najmniej w ziarnie pszenicy odpowiednio 16,11 mg/kg i 4,13 mg/kg.

7

Oznaczanie ergosterolu w produktach skazonych patogennymi grzybami i mikotoksynami.

84%

Grajewski J., Pettersson H.

Zeszyty Naukowe Wyższej Szkoły Pedagogicznej w Bydgoszczy. Studia Przyrodnicze

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1997

|

tom 13

125-134

8

Wplyw deszczowania na kolonizacje mikoryzowa i zawartosc ergosterolu w korzeniach siewek sosny zwyczajnej [Pinus sylvestris L.]

84%

Hilszczanska D.

Prace Instytutu Badawczego Leśnictwa. Seria A

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2003

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nr 4[960-965]

55-64

9

Odporność rodów hodowlanych pszenżyta ozimego na infekcję kłosa grzybem Fusarium culmorum

84%

Wiśniewska H., Kwiatek M., Belter J., Majka M., Góral T., Ochodzki P., Walentyn-Góral D., Banaszak Z., Pojmaj M., Kurleto D., Konieczny M., Budzianowski G., Cicha A., Paizert K., Woś H.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2015

|

nr 276

10

Akumulacja deoksyniwalenolu i ergosterolu w ziarnie pszenzyta porazonym przez Fusarium culmorum

84%

Busko M., Goral T., Cichy H., Matysiak A., Perkowski J.

Folia Universitatis Agriculturae Stetinensis. Agricultura

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2006

|

tom 100

21-28

11

Wplyw substancji lipidowych na przebieg fermentacji etanolowej

84%

Grajek W., Gliszczynska A., Walkowiak-Tomczak D.

Roczniki Akademii Rolniczej w Poznaniu. Technologia Żywności

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1995

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tom 19

|

nr 1

49-57

12

Ergosterol content in dust created during working of wood and wood composites

84%

Szwajkowska-Michalek L., Rogozinski T., Dolny S., Perkowski J.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2013

|

tom 81

13

Influence of N,N-bis[3-aminopropyl]dodecylamine on the mycelium growth and the cell wall composition of resistance and sensitive strains belonging to the genus Aspergillus

84%

Kozirog A., Kuberski S., Zakowska Z., Brycki B.

Polish Journal of Microbiology

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2005

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tom 54

|

nr 4

Resistance causes of moulds to N,N-bis(3-aminopropyl)dodecylamine (APDA) for selected species of Aspergillus niger and Aspergillus flavus was examined. Control (sensitive) strains and resistant strains, cultured at 0.05% triamine, were used in the experiments. The non-resistant strains did not have growth capacity in this amount of ADPA while the resistant strains were characterised by a smaller biomass increase. Individual stages of the development of the mycelium occurred later than those in the control samples. The participation of the cell wall in the mycelium biomass of the resistant strains was higher by 7.5%. The glucan content in the wall dry mass was lower by 11% than that in the sensitive strains. A 41% increase in the lipid content was recorded in the cell wall of resistant Aspergillus flavus. A 21 % protein increase occurred in the wall of Aspergillus niger comparing to the control strain. Infrared spectrophotometry analysis of the cell wall did not reveal the presence of triamine. Most absorption bands disappeared in the wall of Aspergillus flavus while no additional absorption bands were registered in Aspergillus niger, some bands were only stronger than those in the control sample. The resistant strains were characterised by a smaller ergosterol content, the main constituent of cell membranes. Spectrophotometry analysis of the mycelium did not reveal significant qualitative changes; only quantitative changes were observed. It was noticed that the resistance reaction did not occur with the same intensity in both species studied. The resistant strain of Aspergillus niger was characterised by a slightly more intensive absorption within its entire spectrum range in comparison to control strain. In case of Aspergillus flavus the absorption was higher for control strain.

14

Molecular modelling of membrane activity of amphotericin B, a polyene macrolide antifungal antibiotic

84%

Baginski M., Sternal K., Czub J., Borowski E.

Acta Biochimica Polonica

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2005

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tom 52

|

nr 3

Amphotericin B (AmB) is a well known polyene macrolide antibiotic used to treat systemic fungal infections. Despite its toxicity AmB is still regarded as a life-saving drug. The lack of adequate knowledge of the AmB mechanism of action is a serious obstacle to efficient development of new less toxic derivatives. Complementary to various experimental approaches, computational chemistry methods were used to study AmB mechanism of action. A programme lasting for a decade, that was run by our group covered studies of: i) molecular properties of AmB and its membrane targets, ii) structure and properties of AmB membrane channels, and iii) interaction of AmB with the membrane.

15

Effect of aluminium on Pinus sylvestris seedlings mycorrhizal with aluminium-tolerant and aluminium-sensitive strains of Suillus luteus

84%

Rudawska M., Kieliszewska-Rokicka B., Leski T.

Dendrobiology

|

2000

|

tom 45

Mycorrhizal syntheses of pine seedlings were conducted with Suillus luteus (L.) S.F. Gray, a strain No 14 characterised by high tolerance to Al3+ ions and a strain No 62, sensitive to aluminium. The experiment was performed as a semi-sterile culture in a peat-perlite medium with Al3+ ion concentration of 11 mM. Abundant coralloid and cluster mycorrhizas of S. luteus were formed on roots of the inoculated plants at the beginning of the experiment. Aluminium treatment limited mycorrhizal morphotypes to single and dichotomous and significantly reduced the total number of mycorrhizal tips but had no effect on extramatrical mycelium development in the potting substrate. Al treatment did not affect growth of the above-ground part of the tested plants but significantly reduced root growth of mycorrhizal seedlings. The effect of Al on the internal nutrient status was variable and not very much pronounced. A considerable amount of Al was absorbed by the roots and translocated to the shoots. Mycorrhiza formation with both strains of S. luteus did not prevent Al3+ translocation to the upper parts of the tested seedlings. The results suggest that low pH and high Al availability may harmfully influence mycorrhizal symbiosis of Scots pine (Pinus sylvestris L.) by anega tive effect on fine-root production and fewer short root tips available for colonisation rather than through a direct negative effect of Al3+ ion concentrations on extramatrical mycelium in the soil.

16

Rsp5 ubiquitin ligase affects isoprenoid pathway and cell wall organization in S. cerevisiae

84%

Kaminska J., Kwapisz M., Grabinska K., Orlowski J., Boguta M., Palamarczyk G., Zoladek T.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 1

Dimethylallyl diphosphate, an isomer of isopentenyl diphosphate, is a common substrate of Mod5p, a tRNA modifying enzyme, and the farnesyl diphosphate synthase Erg20p, the key enzyme of the isoprenoid pathway. rsp5 mutants, defective in the Rsp5 ubiquitin-protein ligase, were isolated and characterized as altering the mitochondrial/cytosolic distribution of Mod5p. To understand better how competition for the substrate determines the regulation at the molecular level, we analyzed the effect of the rsp5-13 mutation on Erg20p expression. The level of Erg20p was three times lower in rsp5-13 compared to the wild type strain and this effect was dependent on active Mod5p. Northern blot analysis indicated a regulatory role of Rsp5p in ERG20 transcription. ERG20 expression was also impaired in pkc1Δ lacking a component of the cell wall integrity signaling pathway. Low expression of Erg20p in rsp5 cells was accompanied by low level of ergosterol, the main end product of the isoprenoid pathway. Additionally, rsp5 strains were resistant to nystatin, which binds to ergosterol present in the plasma membrane, and sensitive to calcofluor white, a drug destabilizing cell wall integrity by binding to chitin. Furthermore, the cell wall structure appeared abnormal in most rsp5-13 cells investigated by electron microscopy and chitin level in the cell wall was increased two-fold. These results indicate that Rsp5p affects the isoprenoid pathway which has important roles in ergosterol biosynthesis, protein glycosylation and transport and in this way may influence the composition of the plasma membrane and cell wall.

17

Oznaczanie ergosterolu w probach biologicznych za pomoca chromatografii cienkowarstwowej

83%

Wlodarczyk E., Zarzycki P. K.

Bromatologia i Chemia Toksykologiczna

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2005

|

tom 38

|

nr 4

361-369

18

Wplyw inokulacji klosow roznych odmian oraz linii pszenzyta ozimego Fusarium culmorum [W.G.Sm.] Sacc. na zawartosc ergosterolu i mikotoksyn w ziarnie

71%

Busko M., Goral T., Kostecki M., Cichy H., Jackowiak H., Perkowski J.

Progress in Plant Protection

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2002

|

tom 42

|

nr 2

836-839

19

Wystepowanie ergosterolu w runi uzytkow zielonych w zaleznosci od czasu jej odrastania i terminu zbioru

71%

Golinski P., Opitz von Boberfeld W., Golinska B.

Progress in Plant Protection

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2005

|

tom 45

|

nr 2

672-674

20

Factors determining the sward utilisation of winter pasture in cattle feeding

67%

Golinski P., Golinska B., Golinska B. T., Golinski P. K.

Polish Journal of Veterinary Sciences

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2008

|

tom 11

|

nr 3

257-262

In this review article, factors determining the sward utilisation of winter pasture in cattle feeding were defined and analyzed. The results from literature and own investigations have shown that yield and quality of autumn-saved herbage on winter pasture are determined by pratotechnical measures such as harvest date in winter and pre-utilisation date in summer, botanical composition of sward, and particularly the dominated grass species or community, nitrogen fertilisation and weather conditions in winter. The date of winter harvest as a dominating factor affects the development of dry matter yield as well as the digestibility of organic matter and nutrients concentration in herbage during winter. For the management of winter grazing systems it could be obtained that crude protein and energy concentration of the tested autumn-saved herbage met the requirements of suckler cows or beef cattle until the end of the year if they were pre-utilised in July. The weather conditions during autumn-winter period are important factor determining the accumulation of secondary metabolites formed by field fungi in herbage of winter pasture.

Ograniczanie wyników

Contamination with microscopic fungi measured by the concentration of ergosterol in dusts of various types of wood with different granulation

Fungi biomass in lowland rivers of North-Eastern Poland: effects of habitat conditions and nutrient concentration

Comparison of various methods for determination of antifungal activity of Bacillus coagulans

Ergosterol biosynthesis inhibition: a target for antifungal agents

Ergosterol jako wskaznik mikroflory grzybowej ze szczegolnym uwzglednieniem jego zawartosci w zbozach oraz produktach zbozowych

Zawartosc ergosterolu w zbozowych produktach spozywczych

Oznaczanie ergosterolu w produktach skazonych patogennymi grzybami i mikotoksynami.

Wplyw deszczowania na kolonizacje mikoryzowa i zawartosc ergosterolu w korzeniach siewek sosny zwyczajnej [Pinus sylvestris L.]

Odporność rodów hodowlanych pszenżyta ozimego na infekcję kłosa grzybem Fusarium culmorum

Akumulacja deoksyniwalenolu i ergosterolu w ziarnie pszenzyta porazonym przez Fusarium culmorum

Wplyw substancji lipidowych na przebieg fermentacji etanolowej

Ergosterol content in dust created during working of wood and wood composites

Influence of N,N-bis[3-aminopropyl]dodecylamine on the mycelium growth and the cell wall composition of resistance and sensitive strains belonging to the genus Aspergillus

Molecular modelling of membrane activity of amphotericin B, a polyene macrolide antifungal antibiotic

Effect of aluminium on Pinus sylvestris seedlings mycorrhizal with aluminium-tolerant and aluminium-sensitive strains of Suillus luteus

Rsp5 ubiquitin ligase affects isoprenoid pathway and cell wall organization in S. cerevisiae

Oznaczanie ergosterolu w probach biologicznych za pomoca chromatografii cienkowarstwowej

Wplyw inokulacji klosow roznych odmian oraz linii pszenzyta ozimego Fusarium culmorum [W.G.Sm.] Sacc. na zawartosc ergosterolu i mikotoksyn w ziarnie

Wystepowanie ergosterolu w runi uzytkow zielonych w zaleznosci od czasu jej odrastania i terminu zbioru

Factors determining the sward utilisation of winter pasture in cattle feeding