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The first Aujeszky's disease (AD) vaccination - eradication programme in Poland was introduced in two different in size farrow-to-finish farms with a high percentage of latently infected pigs. All the pigs were vaccinated with gE- and TK-negative vaccine Nobi-Porvac Aujeszky Live Begonia (Intervet, Holland) according to the following schedule: breeding stock twice with a 4-week interval and then 3 times yearly, replacement gilts at 10-12 weeks of age, 4 weeks later and then together with sows and boars, fattening pigs twice at 10-12 weeks of age and 4 weeks later. In both herds clinical signs, postvaccinal humoral immune response as well as gE-seroprevalence were examined. After introduction of the vaccination programme no clinical signs of AD and neither local nor general adverse effects in pigs were observed. In the sows tested the vaccination schedule enabled high levels of neutralizing antibodies to be maintained. In the fattening pigs born to the vaccinated sows low antibody levels after the first and regular booster effect two weeks after the second vaccination were observed. An intensive vaccination programme is a good tool to minimize AD virus infections in pigs and, in conjunction with normal breeding stock turnover, strongly reduces the prevalence of gE-seropositive breeding and fattening pigs.
We analysed 251 early spring and winter storage honey samples, individually collected from the apiaries of 9 districts of Małopolska province in South Poland and revealed that 51 of these specimens were contaminated with different levels of P. larvae larvae spores Among these samples 8.8% were classified as a category I contamination and 11.5% as a category II - reperesenting 22 (43.1%) and 29 (56.9%) of the total positives, respectively. We conclude from these analyses that: (1) - the total number of new outbreaks of AFB in Poland are most likely to be higher than previously reported, (2) - the quantitative examination of samples of winter stored or early spring honeys for the presence of P. larvae .larvae spores can improve the detection rate of AFB outbreaks, (3) - the high percentage of apiaries that were found to be free of P. larvae larvae (80%) may facilitate the implementation of an AFB eradication programme on a large scale in Poland.
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