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The observation of the rapid rate of plant cover transformation caused by urbanization and agricultural technology was the inspiration for undertaking the study in rural areas. The study was carried out in the Sandomierz Basin in four typical villages with varying degrees of the anthropogenic impact. The changes observed included: Koszyce Małe – evident transformation of fields and meadows into building land; Kolbuszowa Dolna – intensive changes in land use, abandonment of fields and meadows, and river regulation; Roźwienica – emergence of new habitats for plant colonization after reclamation of land previously occupied by a brick factory and a landfill; Krzeczowice – intensive crop cultivation and unchanged land use. In 2009–2010, floristic investigations with the patrol method were carried out in these areas. The historical-geographical classification and indices of the anthropogenic changes of the flora were employed in the analysis. Substantially, domination of synanthropic species over non-synanthropic spontaneophytes was noted in the analysed floras. In the synanthropic species group, the proportion of apophytes was 3-fold higher than that of anthropophytes. Archaeophytes were more abundant than kenophytes. The flora of Krzeczowice, an area affected by long-term anthropopressure, was by approx. 30% less abundant than that in the other villages; it was also characterised by the highest synanthropization index, apophytization index, and index of apophytism of spontaneophytes. In turn, the flora found in Kolbuszowa Dolna was rich due to the presence of a wide variety of habitats; it was characterised by the lowest apophytization index accompanied by the highest anthropophization index.
Epiphytes and hemi-epiphytes are important floristic, structural and functional components of tropical rainforests. Their specific responses to light, temperature and water conditions during seed germination allow them to coexist with tropical forest trees. Here we investigated the effects of temperature, red to far-red light ratio (R:FR ratio) and water stress on seed germination of Ficus virens in tropical seasonal rainforest in Southwest China. We used incubators to create required temperature regimes, polyester filters to produce R:FR ratio gradients and mannitol solutions to simulate water stress. It was found that seed germination of F. virens was inhibited in the simulated understory conditions, i.e., at lower temperature (22/23°C), especially when combined with the R:FR ratio of 0.25, for which the germination percentage was less than 20%. In contrast, the seed germination percentages in the simulated canopy environment (22/32°C) showed no significant difference between R:FR ratios, with an average seed germination percentage as high as 65.8%. Seed germination delayed and decreased along with increasing water stress and was completely inhibited at -2.5 MPa, which might suggest that it is a kind of adaptation for F. virens seeds to detect the rainy season as germination chance on the canopy. Therefore, our study revealed the physiological mechanism for F. virensto be able to adapt to canopy environment.
The present investigation was carried out to analyse the presence of endophytes in the above-ground parts of Hipericum perforatum and to analyse the biodiversity and enumeration of epiphytes. Plant material was collected in Poland three times during the growing season. Phenotypic and genotypic diversity of all the endophytes and the most abundant epiphytes were researched. We analysed fungistatic activity of this isolates. From the endosphere of tested plant Alcaligenes faecalis and Bacillus licheniformis were isolated. The most numerous epiphytes were the copiotrophs and a bit less numerous were oligotrophs, bacteria cultivated on Bunt and Rovir’s medium and fungi. The least numerous bacteria were Azotobacter sp. Among all the molds dominant were: Cladosporium herbarum, C. cladosporioides and Alternaria consortialis, A. alternata, Clonostachys rosea f. catenulata (Gliocladium catenulatum), Scopulariopsis brevicaulis and Penicillium terrestre. Among phyllobacteria there were found mostly the following species: Burkholderia cepacia, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas fluorescens, P. putida, Pantoea agglomerans, Paenibacillus polymyxa, Bacillus cereus, Rhodococcus sp., R. erythropolis and Cellulosimicrobium cellulans. The broadest spectrum of antifungal activity was examined for the following species: Paenibacillus polymyxa, Pseudomonas putida and Pantoea agglomerans. P. polymyxa limited the growth of over 82% tested molds, so did the other two strains: P. agglomerans over 77% and P. putida over 73%.
The agricultural based economy is a core business in Thailand and food export is one of the main sources of income for the Thai population. However, pesticides are overused and misused. As a result there is an urgent need to reduce the use of synthetic chemicals. Biological control offers an alternative to the use of pesticides. Mango (Mangifera indica L.) is widely planted in Thailand and is one of the major cash crops for international export. However, mango suffers from various diseases especially anthracnose, a fungal disease caused by Colletotrichum gloeosporioides. One hundred and twelve isolates of epiphytic microbes were isolated from healthy leaves and fruits of mangoes; this included 93 and 19 isolates of epiphytic bacteria and yeasts, respectively. They were screened for bioactivity against a pathogenic strain of C. gloeosporioides isolated from diseased mangoes using a dual culture technique. Out of 112 isolates, eight isolates exhibited at least 60% inhibition. These isolates were further screened for their inhibition on mango using fruit inoculation. Two isolates reduced the lesion sizes caused by C. gloeosporioides compared to control treatment. These two isolates, based on phenotypical and biochemical tests, were identified as Bacillus sp. MB61 and Bacillus sp. LB72.
W latach 2009-2011 w warunkach polowych analizowano liczebność zbiorowisk epifitycznych i endofitycznych bakterii z grupy pseudomonad oraz Azotobacter spp. oraz grzybów drożdżoidalnych i strzępkowych zasiedlających liście pszenicy ozimej odmiany Bogatka. Populacje endofitycznych drobnoustrojów uzyskane z liści pszenicy ozimej były liczniejsze niż epifity. Liczebność mikroorganizmów epifitycznych podlegała silnym fluktuacjom w zależności od terminu pobierania prób. Odnotowano bardzo silną ujemną korelację między liczebnością analizowanych epifitycznych bakterii a zdrowotnością dolnych liści oraz silną dodatnią korelację między liczebnością epifitycznych grzybów strzępkowych a porażeniem dolnych liści patogenami rodzaju Septoria.
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