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The present study was aimed to assess the antihyperlipidemic, antihypertensive and antioxidant effect of D-carvone, a monoterpene against Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) induced hypertension. Hypertension was prompted in adult male albino rats of the Wistar strain by oral administration of the L-NAME (40 mg/kg body weight) in drinking water for 4 weeks. Rats were treated with D-carvone (5, 10 and 20 mg/kg body weight) for four weeks. L-NAME treated rats exhibited significant increase in water intake, heart rate, aortic lipids level such as triglycerides (TG), total cholesterol (TC), free fatty acids (FFA) and significant decrease in the level of phospholipids (PL), plasma nitric oxide (NO). The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were decreased in erythrocytes of L-NAME induced hypertensive rats. Treatment with D-carvone restored all the above parameters to near normal level. These results suggest that D-carvone acts as an antihyperlipidemic, antihypertensive and antioxidant agent against L-NAME induced hypertensive rats.
The aim of the study was to evaluate oxidative stress in cows undergoing normal parturition and cows suffering from dystocia. The erythrocytic glutathione peroxidase (GSH-Px) activity, plasma vitamin A and ß-carotene concentrations, and paraoxonase - PONI activity were lowered (P0.001, P<0.01, 0.05, and 0.05, respectively) in cows with dystocia compared to normal calving cows. The erythrocytic malondialdehyde (MDA) concentration was markedly increased in the dystocia group compared to normal calving group. However, erythrocytic glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) activites, and plasma vitamin E and MDA concentrations were not significantly changed in the dystocia-affected cows in comparison to eutocia cows. In the difficult calving cows, there were significant correlations between the activities of GSH-Px and SOD (r=-0.4l, P<0.01), plasma ß-carotene levels and paraoxonase activity (r=0.34, P<0.05), body condition score (BCS) and plasma MDA (r=0.46, P<0.05). Similarly in the eutocia cows there were significant correlations between CAT activity and MDA concentration (r=-0.76, P<0.01), levels of plasma ß-carotene and PONI (r=0.58, P<0.01), BCS and MDA concentrations (r=0.50, P<0.05), and BCS and vitamin E (r=0.53, P<0.05) concentrations. These results suggests that evaluation of plasma vitamin A and ß-carotene concentrations, PONI and GSH-Px activities, and MDA concentration seems to be useful in the assessment of dystocia in cows.
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Lymphocyte DNA damage in rats challenged with a single bout of strenuous exercise

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Exercise induces extensive generation of reactive oxygen species, which are responsible for tissue damage: enzymes inactivation, lipid peroxidation and single strand breaks in DNA. Defense system against free radicals is consisting of enzymes such as superoxide dismutase, catalase, glutathione peroxidase, and numerous non-enzymatic antioxidants. The study was performed to evaluate the effect of a single bout of submaximal running exercise, on the lymphocyte DNA strand breaks and also to test how supplementation with tempol – a membrane-permeable SOD-mimetic (0.2 mmol/kg/day) influences the eventually evoked damage. Male, Wistar rats were challenged with graded 50 min. running on treadmill at intensity up to 75-85% of predicted O2 max. The DNA strand breaks in individual lymphocytes were determined by using a gel electrophoretic technique – “comet” assay. We found substantial lymphocyte DNA damage 60 min. after the exercise. Tempol failed to prevent from oxidative damage in rats challenged with exercise. Moreover tempol by itself induced higher DNA damage than the exercise bout.
Chlorfenvinphos, 2-chloro-1-(2,4-dichlorofenyl)vinyl diethyl phosphate, is an organophos-phate insecticide widely used in Poland singly or in mixture. The present study was undertaken to determine chlorfenvinphos-induced lipid peroxidation and to show whether acute intoxication with chlorfenvinphos alters the antioxidant system in the erythrocytes and serum. The study was conducted on male Wistar rats divided into two groups. The animals of control group were given olive oil intragastrically by a stomach tube, the animals of experimental group received oil solution of chlorfenvinphos (CVP) at a dose of 0.02, 0.1 or 0.5LD50. The blood was collected via heart puncture at the 1st, 24th and 48th hour after treatment. We determined the erythrocytes concentration of glutathione, the activities of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase as well as the serum concentration of ascorbic acid, á-to-copherol and malondialdehyde. We observed the stimulation of enzymatic and nonenzymatic antioxi-dant system and lipid peroxidation in the erythrocytes and serum of chlorfenvinphos intoxicated rats.
Cell metabolism in organisms which use oxygen as a source of energy is closely associated with the generation and action of free oxygen radicals and their derivatives. Extra- and intracellular substances that are antioxidative in nature prevent overproduction of radicals and protect against propagation of peroxidative reactions. The list of compounds which can be treated as antioxidants becomes elongated. Many classifications of these compounds are used, of which the most common is the division according to their nature into enzymatic and non-enzymatic, according to their environment or the way they react with FOR. Enzymatic antioxidants include: superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. Non-enzymatic antioxidants are: vitamin E, vitamin C, glutathione, carotenes and retinols, and some transition metals (Zn, Cu and Se). The balance between the actions of these two groups of compounds determines normal functioning of the organism. Impairment of the balance between pro- and antioxidative processes in the organism is called anitoxidative stress and may be induced by intensified reactions involving FOR and by depressed activity/concentration of antioxidants. It seems, however, that irrespective of the cause, oxidative stress is likely to result in many diseases.
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