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Effect of enterocin a on the intestinal epithelium of Japanese quails infected by Salmonella Duesseldorf

100%

Cigankova V., Laukova A., Guba P., Nemcova R.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 1

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Effect of enterocin 4231 in Slovak fermented salami Puchov after its experimental inoculation with Listeria innocua Li1

80%

Laukova A., Turek P.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2011

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tom 10

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nr 4

Background. Enteroccocci occur and may compete well in fermented sausages and Enterococcus faecium represents that species of the lactic acid bacteria which can be found in the fermented sausages. The representatives of this species can produce bacteriocins with predominant anti-listerial effect. Therefore, the effect of enterocin (Ent) 4231 produced by Enterococcus faecium CCM 4231 strain with probiotic properties was tested in a dry fermented salami Puchov (Slovak product) experimentally inoculated with L. innocua Li1 strain (107 cfu/ml). Material and methods. The bulk salami mixture was prepared in the pilot plant and 2.5 kg for each of three trials were transferred to the laboratory for the experiments. Three independent trials were conducted, each comprising then five salami samples (0.500 g). Trial A (reference control) involved only untreated salami mixture. Trial B represented salami mixture inoculated with Listeria innocua Li1 (107 cfu/ml). For trial C, Ent 4231 possessing activity 6400 AU/ml was added into the salami mixture inoculated with L. innocua Li1 (LilEnt). The mixtures were stuffed into collagen casings and the fiat shape salamis were transferred back to the pilot plant and treated according to conditions typical for this product and stored for 4 weeks. Results. The initial number of L. innocua Li1 in the inoculated salami mixture was 104 cfu/ml. Aft er Ent 4231 addition, the count of Listeria detected in the salami samples inoculated with Li1 and treated with Ent 4231 was 3.64 ±0.14 cfu/ml; difference 0.40 logarithmic cycles was noted between Li samples and Li /Ent samples. On day 2, the difference 1.86 log cycles was noted between Li1 and IM Ent samples. Although, in weeks 3 and 4, slight increase in Li1 cells was determined in Li salamis, the difference in the detection of Li1 cells in Li salamis and Li/Ent samples was even higher than that immedially after Ent addition (difference 2.30; 2.48 log cycles). Bacteriocin activity itself was not recovered from Li/Ent salamis. The pH of the all salamis was almost at the same level. Water activity and water content were not influenced. Conclusion. Addition of Ent 4231 during processing of salami Puchov experimentally inoculated with L. innocua Li1 has lead to decrease of Li1 cell growth, although the bacteriocin activity of Ent itself was not possible to detect in salami samples. The pH value, water activity, as well as sensory character of the final products were not negatively influenced.

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Characteristic of bacteriocines and their application

80%

Karpinski T. M., Szkaradkiewicz A. K.

Polish Journal of Microbiology

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2013

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tom 62

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nr 3

Bacteriocines are small peptides with anti-bacterial properties. They are produced both by Gram-positive and Gram-negative bacteria. Until now, a few hundred bacteriocines were described. Classification of bacteriocines undergoes continuous alterations, as new developments regarding their structure, amino acid sequence and recognised mechanism of their action are available. Some of bacteriocins (lantibiotics) contain atypical amino acids, such as lantionine (Lan), methyllantionine (MeLan), dehydroalanine (Dha), dehydrobutyrine (Dhb), or D-alanine (D-Ala). The best recognized bacteriocines are produced by lactic acid bacteria, including nisine produced by strains of Lactococcus lactis. These bacteriocines have been recognized to be fully safe for humans. At present, nisine is used in food industry, as a preserving agent. Other lactic acid bacteria bacteriocines and probiotic preparations provide an alternative for antibiotics, and are used in food and in animal feed.

Ograniczanie wyników

1 Acta Scientiarum Polonorum. Technologia Alimentaria

1 Bulletin of the Veterinary Institute in Puławy

1 Polish Journal of Microbiology

2 Laukova A.

1 Cigankova V.

1 Guba P.

1 Karpinski T. M.

1 Nemcova R.

1 Szkaradkiewicz A. K.

1 Turek P.

1 2013

1 2011

1 2004

Effect of enterocin a on the intestinal epithelium of Japanese quails infected by Salmonella Duesseldorf

Effect of enterocin 4231 in Slovak fermented salami Puchov after its experimental inoculation with Listeria innocua Li1

Characteristic of bacteriocines and their application