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1

Coccidiosis of small ruminants in various regions of Slovakia

100%
Vasilkova Z., Krupicer I., Legath J., Kovalkovicova N., Pet'ko B.
Acta Parasitologica
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2004
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tom 49
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nr 4
Coccidian oocysts were prevalent in nearly 100% of goats and sheep from various localities in Slovakia. In goats 4 Eimeria species were identified: E. ninakohlyakimovae (in kids 40%, in adult goats 51 %), E. arloingi (in kids 43%, in adult goats 20%), E. alijevi (in kids 12%, in adult goats 21 %) and E. hirci (in kids 3%, in adult goats 2%). In addition, 2-6% of coccidian oocysts were not speciated. The mean number of oocysts per gram of faeces (OPG) was 5853 ± 12666 (min. 160-max. 31920) in kids and 2365 ± 4916 (min. 80 - max. 7920) in adult goats. In sheep 5 Eimeria species were identified: E. parva (in lambs 42%, in adult sheep 37%), E. ovinoidalis (in lambs 33%, in adult sheep 29%), E. crandallis (in lambs 14%, in adult sheep 19%), E. bakuensis (in lambs 6%, in adult sheep 6%), E. faurei (in lambs 3%, in adult sheep 4%); 2-5% of coccidian oocysts could not be classified. The mean OPG value was 11941 ±9048 (min. 2680-max. 48880) in lambs and 5250 ± 3412 (min. 880-max. 12280) in adult sheep. In connection with the occurrence of pathogenic Eimeria species, the total counts of selected enterobacteriae in faeces of both goats and sheep were also evaluated. In spite of the fact that in kids the mean OPG numbers were lower, the values of total bacterial counts in their faeces were 1-2 orders of magnitude higher than in lambs. In comparison with lambs, organisms of kids are probably more susceptible to influence of pathogenic Eimeria species. The total counts of selected enterobacteria genera in goats and sheep with coccidiosis were higher than in control groups. These increased bacterial counts might be affected by impaired immunity of the host. Other factors as feeding, breeding conditions and management etc. may be also taken into consideration.
2
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Involvement of corticotropin-releasing factor and corticotropin-releasing factor 2 receptors in pathogenesis of ischemia/reperfusion-induced enteritis in rats

84%
Takeuchi K., Kumano A., Abe N., Kotani T.
Journal of Physiology and Pharmacology
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2016
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tom 67
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nr 5
3

Antibiotic sensitivity and sequence amplification patterns of genes in multidrug resistant Enterobacteria isolates from processed foods in some West African countries

67%
Owoseni A. A., Onilude A. A.
Polish Journal of Microbiology
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2011
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tom 60
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nr 4
Diarrhoea, dysentery and other diseases due to other enteric bacteria have reportedly been found to resist chemotherapeutic treatment in some West African communities with fatal consequences in some cases. This study was carried out to determine multidrug resistance patterns of Enterobacteria isolates from processed ready-to-eat foods. Indigenously processed food samples of different types were collected from two Francophone and two Anglophone countries in the West African sub-region during the wet and dry seasons of a sampling period of two years. Enterobacteria were isolated from the samples using standard techniques. Amplification of chromosomal DNA of the isolates using the Polymerase Chain Reaction was carried out. The results obtained were subjected to statistical analyses. All isolates showed resistance to cefuroxime (90.7%), nitrofurantoin (90.6%), augmentin (86.1%) and ampicillin (51.2%) while all were sensitive to gentamycin and ciprofloxacin. There was amplification indicating the presence of invA gene at a position of 240 bp. There was no amplification at all for the spvC gene in any of the isolates tested. Multidrug resistant enteric bacteria in these foods containing the invA gene could lead to infections with uncontrolled antibiotic use. The presence of enteric bacteria in the foods analyzed which provide undeniable evidence of the poor microbiological quality of these foods could form the basis of a useful databank in formulation of food-borne disease control and prevention strategies.
4

Comparative study of microcalorimetric behavior of Escherichia coli, Proteus mirabilis and Klebsiella pneumoniae

67%
Rivero N. L., Legido J. L., Santos I. A., Casas L. M.
Polish Journal of Microbiology
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2012
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tom 61
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nr 3
To analyze the microcalorimetric behaviour of Escherichia coli, Proteus mirabilis and Klebsiella pneumoniae, and to test the prospective use of microcalorimetry in the early diagnosis of infections by enterobacteria. For our experiments we used cultures of E. coli, P. mirabilis and K. pneumoniae. For the experimental setup, we used a Calvet microcalorimeter with the constant temperature of 309 K inside. Using the measured difference of heat power generated by the cells over time, we obtained growth graphs of the three bacterial species grown at different densities. !e generated curves have characteristic plot that repeats for each bacterial species at all the concentrations studied. We observed an inversely proportional relationship between inoculum size and the time until signal detection; the smaller inoculum, the longer time for the signal detection. In all conducted experiments, even with low number of bacteria (10 CFU/ml) as inoculum, we could identify bacterial growth in the sample within 8 hours. Microcalorimetry could be an efficient technique used for the early detection and identification of enterobacteria in a culture medium.
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