Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Stomata and ploidy level differentiation of plants obtained from Actinidia deliciosa var. deliciosa endosperm culture

100%

Kleszcz I., Sliwinska E., Popielarska-Konieczna M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

2

Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro

100%

Goralski G., Popielarska M., Slesak H., Siwinska D., Batycka M.

Acta Biologica Cracoviensia. Series Botanica

|

2005

|

tom 47

|

nr 2

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were significant differences in regeneration response between medium supplemented with TDZ and medium with other hormones. Only medium containing TDZ stimulated shoot induction. The highest efficiency of shoot regeneration (avg. 6.2 shoots per culture) was on medium supplemented with 0.5 mg/l TDZ. The ploidy of callus and organs formed in endosperm culture was examined by flow cytometry. The results, peaks corresponding to 3C DNA amounts, confirmed the endospermal origin of callus, roots and shoots. Aneuploid and polyploid cells were found in endosperm-derived callus and regenerated organs.

3

Histological and SEM studies on organogenesis in endosperm-derived callus of kiwifruit [Actinidia deliciosa cv. Hayward]

80%

Popielarska M., Slesak H., Goralski G.

Acta Biologica Cracoviensia. Series Botanica

|

2006

|

tom 48

|

nr 2

Regeneration in endosperm-derived callus of Actinidla deliciosa cv. Hayward was documented by SEM combined with histology. Two kinds of callus, morphogenic and nonmorphogenic, were observed. Morphogenic callus consisted of compact cell clusters with epidermis-like tissue covered with a mucilaginous or continuous membranous layer, which partially disappeared, turned into fibrils, or became damaged. Regenerating shoots consisted of the apex and primordial leaves. Abnormal structures were also formed, frequently arrested in development. PAS reactions indicated that the mucilaginous layer and network present in intercellular spaces contains polysaccharides. Nonmorphogenic callus consisted of weakly attached cells without a covering membranous layer.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2 Goralski G.

2 Popielarska M.

2 Slesak H.

1 Batycka M.

1 Kleszcz I.

1 Popielarska-Konieczna M.

1 Siwinska D.

1 Sliwinska E.

1 2015

1 2006

1 2005

Stomata and ploidy level differentiation of plants obtained from Actinidia deliciosa var. deliciosa endosperm culture

Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro

Histological and SEM studies on organogenesis in endosperm-derived callus of kiwifruit [Actinidia deliciosa cv. Hayward]