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Effect of TAT-signaling fusion system along with co-expression of GroEL/ES chaperones on secretory expression of somatropin

100%
Rabbani M., Ghasemi R., Bagherinejad M. R., Jahanian A.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2020
|
tom 101
|
nr 2
2

Identification of a new restriction endonuclease R.Nci II, from Neisseria cinerea

100%
Piekarowicz A.
Acta Microbiologica Polonica
|
1994
|
tom 43
|
nr 1
3

Molecular cloning and sequencing of the cDNA encoding plant nuclear matrix endonuclease

86%
Markiwicz E., Rzepecki R., Szopa J.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 2
4

The role of DFF40-CAD endonuclease during terminal stages of apoptosis

86%
Widlak P.
Cellular and Molecular Biology Letters
|
2000
|
tom 05
|
nr 2
5

Understanding the evolution of restriction-modification systems: Clues from sequence and structure comparisons

86%
Bujnicki J. M.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 4
Restriction-modification (RM) systems comprise two opposing enzymatic activities: a restriction endonuclease, that targets specific DNA sequences and performs endonucleolytic cleavage, and a modification methyltransferase that renders these se­quences resistant to cleavage. Studies on molecular genetics and biochemistry of RM systems have been carried out over the past four decades, laying foundations for mod­ern molecular biology and providing important models for mechanisms of highly spe­cific protein-DNA interactions. Although the number of known, relevant sequences 3D structures of RM proteins is growing steadily, we do not fully understand their functional diversities from an evolutionary perspective and we are not yet able to en­gineer new sequence specificities based on rational approaches. Recent findings on the evolution of RM systems and on their structures and mechanisms of action have led to a picture in which conserved modules with defined function are shared between different RM proteins and other enzymes involved in nucleic acid biochemistry. On the other hand, it has been realized that some of the modules have been replaced in the evolution by unrelated domains exerting similar function. The aim of this review is to give a survey on the recent progress in the field of structural phylogeny of RM en­zymes with special emphasis on studies of sequence-structure-function relationships and emerging potential applications in biotechnology.
6

Identification of a new restriction endonuclease R.BcrAI, from Bacillus cremoris

86%
Piekarowicz A., Skowronek K.
Acta Microbiologica Polonica
|
1995
|
tom 44
|
nr 3-4
7

Sensitivity of the restriction endonucleases HaeIII, BsrI, EaeI and CfrI to cytosine N4-methylation

72%
Piekarowicz A., Radlinska M.
Acta Microbiologica Polonica
|
1998
|
tom 47
|
nr 4
8

A novel ORF-containing group I intron with His-Cys box in the LSU rDNA of Naegleria

72%
De Jonckheere J. F., Brown S.
Acta Protozoologica
|
2001
|
tom 40
|
nr 1
9

The DFF40-CAD endonuclease and its role in apoptosis

72%
Widlak P.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 4
The sequential generation of large-scale DNA fragments followed by internucleosomal chromatin fragmentation is a biochemical hallmark of apoptosis. One of the nucleases primarily responsible for genomic DNA fragmentation during apoptosis is called DNA Fragmentation Factor 40 (DFF40) or Caspase-activated DNase (CAD). DFF40/CAD is a magnesium-dependent endonuclease specific for double stranded DNA that generates double strand breaks with 3'-hydroxyl ends. DFF40/CAD is activated by caspase-3 that cuts the nuclease's inhibitor DFF45/ICAD. The nuclease preferentially attacks chromatin in the internucleosomal linker DNA. However, the nuclease hypersensitive sites can be detected and DFF40/CAD is potentially involved in large-scale DNA fragmentation as well. DFF40/CAD-mediated DNA fragmentation triggers chromatin condensation that is another hallmark of apoptosis.
10

Immunoglobulins anti-endonuclease 32 kDa from Cucurbita pepo var.patissonina affect process of DNA synthesis

72%
Rzepecki R., Szopa J.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 2
Immunoglobulins anti-endonuclease 32 kDa inhibit DNA synthesis. We observed that low concentrations of IgGs (about 50 jig IgG per 1 x 106 cell nuclei) temporary inhibit DNA synthesis. This inhibition concerns only the synthesis of DNA bound to the nuclear matrix (associated with isolated nuclear matrix). Preincubation of cell nuclei of White bush with IgG generates longer DNA fragments than in controls. Involvement of the 32 kDa endonuclease or an endonuclease-65 kDa protein complex from the nuclear matrix in replication or structural organisation of replication is considered.
11

Interaction of the Pisum sativum nuclear matrix proteins with SAR DNA

72%
Rzepecki R., Markiewicz E., Adamiec R., Szopa J.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 1
We have isolated the nuclear matrices from Pisum sativum cell nuclei using three methods: i. standard procedure involving extraction of cell nuclei with 2 M NaCl and 1% Triton X-100; ii. the same with pretreatment of cell nuclei with 0.5 mM CuS04 (stabilisation step); and iii. method including lithium diiodosalicylate extraction. We compared the polypeptide pattern and residual DNA content of the nuclear matrices isolated. The nuclear matrices displayed a specific endonuclease activity which was due to the presence of a 32 kDa protein. The isolated nuclear matrices bound spe­cifically the scaffold-attached (SAR) DNA derived from human p interferon gene, in the exogenous SAR binding assay. Using the DNA-protein binding blot assay we demonstrated the presence of two nuclear matrix proteins of 66 kDa and 62 kDa which bound specifically SAR DNA.
12

Endogenous and exogenous DNA lesions recognized by N-alkylpurine-DNA glycosylases

72%
Borys E., Kusmierek J. T.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 2
The combined action of glycosylases and abasic site-specific endonucleases on damaged bases in DNA results in single strand breaks.In plasmid DNA, as a consequence, the covalently closed circular (ccc) form is converted to the open circular (oc) form, and this can be quantitated by agarose gel electrophoresis. We studied DNA lesions sensitive to E. coli 3-methyladenine-DNA glycosylase II (AlkA) and cloned human N-alkylpurine-DNA glycosylase (ANPG-40) which are known to excise alkylated bases and etheno adducts. pBR322 and pAlk10 plasmids not pretreated with mutagens were cleaved by both glycosylases in the presence of enzymes possessing endonucleolytic activity, which indicates that plasmids contain unknown, endogenously formed adducts. Plasmids pretreated with chloroacetaldehyde, a mutagen forming etheno adducts, exhibited enhanced sensitivity to both glycosylases. Adducts formed by acrolein and croton aldehyde were excised by AlkA, but not by ANPG-40, whereas malondialdehyde adducts were not excised by either glycosylase. Bulky p-benzochinone adducts were not excised by AlkA, however, the plasmid pretreated with this mutagen was incised by endonucleases, possibly without prior generation of an abasic site. These examples show that examination of conformational changes of plasmid DNA can be taken advantage of to study the specificity of N-alkylpurine-DNA-glycosylases.
13

DFF40-CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis

72%
Widlak P.
Cellular and Molecular Biology Letters
|
2000
|
tom 05
|
nr 3
Sequential cleavage of genomic DNA into large-scale DNA fragments of 50-300-kb, followed by formation of mono- and oligonucleosomal DNA fragments, is a biochemical hallmark of programmed, cell death (apoptosis). The endonuclease DFF40/CAD mediates regulated internucleosomal DNA fragmentation and chromatin condensation in cells undergoing apoptosis. DFF40 hypersensitive sites were detected in purified HeLa cell nuclei, and excision of 50-kb DNA fragments preceded formation of oligonucleosomal DNA ladders in nuclei treated with the nuclease. Topoisomerase II, but not topoisomerase I, stimulates DFF40 activity on plasmid DNA substrates. This suggests that interactions of DFF with the nuclear matrix-bound topoisomerase II may be involved in formation of DFF40 hypersensitive sites.
14

Use of repair endonucleases for characterization of DNA damage induced by N-heterocyclic aromatic hydrocarbons

72%
Gabelova A., Bacova G., Slamenova D., Perin F.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 1
Several repair endonucleases were used to characterize and quantify various types of DNA damage induced by 7H-dibenzo[c,g]carbazole (DBC) and its methyl derivative, N-methyldibenzo[c,g]carbazole (MeDBC). Differences in the DNA damage profile induced by these two derivatives were found to be related to their chemical structure and dependent on the way of their metabolic activation. Different ways of activation gave rise to different numbers of single strand breaks and DNA modifications or, at least, to different ratios of common modifications. DBC induced the highest level of breaks in human hepatal cell line Hep G2, while MeDBC induced most of the breaks in V79 cell line with stable expression of human cytochrome P4501A1. Our results support the idea of two different pathways of biotransformation of DBC and MeDBC.
15

Identification of the proteins responsible for SAR DNA binding in nuclear matrix of Cucurbita pepo

58%
Rzepecki R., Markiewicz E., Szopa J.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 2
The nuclear matrices from White bush (Cucurbita pepovar. patisonina)cell nuclei have been isolated using three methods: I, standard procedure involving extraction of cell nuclei with 2 NaCl and 1% X-100; II, the same with pre-treatment of cell nuclei with 0.5 CuS04 (stabilisation step); and III, method with extraction by lithium diiodosalicylate (LIS), and compared the polypeptide pattern. The isolated matrices specifically bind SAR DNA from human β-interferon gene in the exogenous SAR assay and in the gel mobility shift assay. Using IgG against the 32 endonuclease we have found in the DNA-protein blot assay that this protein is one of the proteins binding SAR DNA. have identified three proteins with molecular mass of 65 , 60 and 32 which are responsible for SAR DNA in the gel mobility shift assay experiments.
16

The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence

58%
Markiewicz E., Wilczynski G., Rzepecki R., Kulma A., Szopa J.
Cellular and Molecular Biology Letters
|
1996
|
tom 01
|
nr 4
The nuclear matrices of plant cell nuclei display an intrinsic nuclease activity which is capable of nicking supercoiled DNA. Recently a cDNA encoding the 14-3-3 protein from Cucurbita pepo has been cloned and sequenced. The evidence that the recombinant 14-3-3 protein associates with DNase I and endogenous plant nuclease is presented. Evidence is also presented that the cloned 14-3-3 protein isoform, unique in its binding to nuclease within the 14-3-3 family, is located in the nuclei and in the nuclear matrix. Transgenic potato plants were created where the 14-3-3 protein derived from Cucurbita was overexpressed. An increase in tuber number and a decrease in tuber size in the transformants was also observed. The adenine nucleotide pool in leaves of transgenic plants was significantly reduced and they contain more chlorophyll and loose it slower when grown in the dark. A decrease in 14-3-3 protein content concomitant with an increase in nuclease activity in senescent plants was detected and this was markedly delayed in transgenic potato plants which overexpressed the 14-3-3 protein. It is proposed that a function of the isolated 14-3-3 isoform is the control of the nuclease activity and hence senescence.
17

Expression analysis of Cucurbita cDNA encoding endonuclease

58%
Szopa J.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 2
The nuclear matrices of plant cell nuclei display intrinsic nuclease activity which consists in nicking supercoiled DNA. A cDNA encoding a 32 kDa endonuclease has been cloned and sequenced. The nucleotide and deduced amino-acid sequences show high homology to known 14-3-3 protein sequences from other sources. The amino-acid sequence shows agreement with consensus sequences for potential phosphorylation by protein kinase A and C and for calcium, lipid and membrane-binding sites. The nucleotide-binding site is also present within the conserved part of the sequence. By Northern blot analysis, the differential expression of the corresponding mRNA was detected; it was the strongest in sink tissues. The endonuclease activity found on DNA-polyacrylamide gel electrophoresis coincided with mRNA content and was the highest in tuber.
18

Length of restriction fragments is a factor determining DNA loss from metaphase chromosomes of Vicia faba

58%
Gernand D., Sakowicz T.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1992
|
tom 40
|
nr 2
19

The isolated 14-3-3 protein isoforms protect DNA against endonuclease[s] digestion in vitro

58%
Rzepecki R., Szalowska E., Klimek A., Wilczynski G., Szopa J.
Cellular and Molecular Biology Letters
|
2000
|
tom 05
|
nr 2
20

Bacterial DNA repair genes and their aukaryotic homologues: 1. Mutations in genes involved in base excision repair [BER] and DNA-end processors and their implication in mutagenesis and human disease

58%
Krwawicz J., Arczewska K. D., Speina E., Maciejewska A., Grzesiuk E.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 3
Base excision repair (BER) pathway executed by a complex network of proteins is the major system responsible for the removal of damaged DNA bases and repair of DNA single strand breaks (SSBs) generated by environmental agents, such as certain cancer therapies, or arising spontaneously during cellular metabolism. Both modified DNA bases and SSBs with ends other than 3'-OH and 5'-P are repaired either by replacement of a single or of more nucleotides in the processes called short-patch BER (SP-BER) or long-patch BER (LP-BER), respectively. In contrast to Escherichia coli cells, in human ones, the two BER sub-pathways are operated by different sets of proteins. In this review the selection between SP- and LP-BER and mutations in BER and end-processors genes and their contribution to bacterial mutagenesis and human diseases are considered.
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