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1

NKT cells: from totipotency to regenerative medicine

100%
Wakao H.
Archivum Immunologiae et Therapiae Experimentalis
|
2009
|
tom 57
|
nr 2
117-128
2

Human embryo stem cells: defining their attributes, cell culture requirements and relevance to large animals

86%
De Sousa P. A., Fletcher J., Campbell C., Harkness L., Ferrier T., Gardner J.
Animal Science Papers and Reports. Supplement
|
2004
|
tom 22
|
nr 1
3

Stem cells in nephrology: present status and future

86%
Watorek E., Klinger M.
Archivum Immunologiae et Therapiae Experimentalis
|
2006
|
tom 54
|
nr 1
4

Gene trapping: An antibody-dependent approach for verifying integration in your favorite gene

72%
Gorelik A., Sapir T., Reiner O.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 4
614-620
Gene trapping is used to introduce genome-wide insertional mutations in embryonic stem cells. Determining the integration site is based on highthroughput PCR, which has inevitable possibilities for mistakes, thus necessitating clone verification prior to the generation of mutant mice. Here, we propose a rapid method to validate gene identity based on the fact that many high throughput gene-trapping integrations result in fusion proteins encompassing the N-terminal portion of the gene of interest and LacZ being expressed in embryonic stem cells. Our method utilizes an immunoprecipitation assay using a specific N-terminal-directed antibody to the protein product of the gene of interest followed by a color LacZ assay of the immunoprecipitate, strongly supporting the formation of a fusion protein when the color develops.
5
Content available remote

Inhibitory effect of octyl-phenol and bisphenol A on calcium signaling in cardiomyocyte differentiation of mouse embryonic stem cells

72%
Lee J.-H., Yoo Y.-M., Jung E.-M., Ahn C., Jeung E.-B.
Journal of Physiology and Pharmacology
|
2019
|
tom 70
|
nr 3
6

Techniques of human embryonic stem cell and induced pluripotent stem cell derivation

72%
Lewandowski J., Kurpisz M.
Archivum Immunologiae et Therapiae Experimentalis
|
2016
|
tom 64
|
nr 5
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Stem cells and their outstanding concerns

72%
Lukomska B.
Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii
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2012
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nr 1
Stem cells have captured considerable scientific and clinic interest because of their potential to renew themselves and to differentiate into one or more adult cell types. Thus stem cells have been recognized as a potential tool for the development of innovative therapeutic strategies in different disease disorders. Stem cells can be discriminating based on their differentiated potential as totipotent, pluripotent, multipotent or unipotent cells. There are in general three types of stem cells: embryonic, fetal and adult stem cells. While embryonic stem cell therapy has a lot of ethical concerns due to their obtaining but also unlimited proliferation and uncontrolled differentiation, fetal and adult stem cells have been used in the treatment of different diseases. The bone marrow, peripheral blood and umbilical cord blood are ideal sources of adult stem cells because there are easily accessible and contain two types of stem cells: hematopoietic stem cells giving rise to all blood cell types and mesenchymal stem cells differentiating into cells of mesodermal lineage. This review describes the general characteristics of these stem cell populations and their current applications in regenerative medicine. Additionally induced pluripotent stem cells generated through the reprogramming of differentiated adult cells are described.
8
Content available remote

Second-phase validation study of an alternative developmental toxicity test using mouse embryonic stem cell-derived embryoid bodies

72%
Lee J.-H., Park S. Y., Ahn C., Yoo Y.-M., Kim C.-W., Kim J.-E., Jo N. R., Kang H. Y., Jung E.-M., Kim K.-S., Choi K.-C., Lee S. D., Jeung E.-B.
Journal of Physiology and Pharmacology
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2020
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tom 71
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nr 2
9

Myogenic stem cells

72%
Burdzinska A., Gala K., Paczek L.
Folia Histochemica et Cytobiologica
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2008
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tom 46
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nr 4
401-412
10

Genetic manipulation of human embryonic stem cells

72%
McWhir J., Didomenico A., Hewitt Z., Priddle H., Thomson A.
Animal Science Papers and Reports. Supplement
|
2004
|
tom 22
|
nr 1
11

Stem cells as therapy for cardiac disease — a review

58%
Jezierska-Wozniak K., Mystkowska D., Tutas A., Jurkowski M. K.
Folia Histochemica et Cytobiologica
|
2011
|
tom 49
|
nr 1
12

Behavior, morphology and morphometry of equine adipose – derived mesenchymal stem cells in culture

58%
Marycz K., Grzesiak J.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2011
|
tom 14
|
nr 2
13

Hybrid cells differentiate to hepatic lineage cells and repair oxidative damage

58%
Xu D., Wang F., Gu H., Wang J., Guo Q., Zhang Y., Wang Z.
Cellular and Molecular Biology Letters
|
2010
|
tom 15
|
nr 3
451-472
Hybrid cells derived from stem cells play an important role in organogenesis, tissue regeneration and cancer formation. However, the fate of hybrid cells and their range of function are poorly understood. Fusing stem cells and somatic cells induces somatic cell reprogramming, and the resulting hybrid cells are embryonic stem cell-like cells. Therefore, we hypothesize that fusion-induced hybrid cells may behave like ES cells in certain microenvironments. In this study, human hepatic cells were induced to apoptosis with H2O2, and then co-cultured with hybrid cells that had been derived from mouse ES cells and human hepatic cells using a transwell. After co-culturing, the degree of apoptosis was evaluated using Annexin-V/PI double-staining analysis, flow cytometry and Western-blot. We observed that H2O2-induced cell apoptosis was inhibited by co-culture. In addition, the activity of injury-related enzymes (GSH-Px, LDH and SOD) and the level of albumin release in the co-culture system trended toward the level of normal undamaged hepatic cells. The stably increased levels of secretion of ALB in the co-culture system also confirmed that co-culture with hybrid cells helped in recovery from injury. The fate of the hybrid cells was studied by analyzing their gene expression and protein expression profiles. The results of RT-PCR indicated that during co-culturing, like ES cells, hybrid cells differentiated into hepatic lineage cells. Hybrid cells transcripted genes from both parental cell genomes. Via immunocytochemical analysis, hepatic directional differentiation of the hybrid cells was also confirmed. After injecting the hybrid cells into the mouse liver, the GFP-labeled transplanted cells were distributed in the hepatic lobules and engrafted into the liver structure. This research expands the knowledge of fusion-related events and the possible function of hybrid cells. Moreover, it could indicate a new route of differentiation from pluripotent cells to tissue-specific cells via conditional co-culture.
14

From embryonic stem cells and induced pluripotent cells to lymphocytes

51%
Seiler K., Tornack J., Karjalainen K., Tsuneto M., Melchers F.
Archivum Immunologiae et Therapiae Experimentalis
|
2011
|
tom 59
|
nr 5
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