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A simple and efficient method for cryopreservation of embryogenic triticale calli

100%

Sowa S., Oleszczuk S., Zimny J.

Acta Physiologiae Plantarum

2005

tom 27

nr 2

Conventional breeding methods are now supplemented by modern in vitro techniques. However, long term maintenance of cultures has many disadvantages. It incurs risk of loss through microbial contamination, somaclonal variation or human error, but above all the regeneration capacity can decrease gradually during extended maintenance. Cryopreservation as a method of long term storage of biological material without genetic alteration was adapted for embryogenic triticale calli preservation. Callus of both winter and spring genotypes were successfully cryopreserved. The best viability rates (80-85 %) were achieved with 6 weeks old winter genotypes treated with cryoprotective solution containing DMSO. This simple and efficient method of cryopreservation requires no special devices for controlled freezing and can be easily adapted for other cereals.

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Apoplastic markers of cell differentiation status during somatic embryogenesis in Arabidopsis thaliana

84%

Potocka I., Sala K., Kurczynska E. U.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2013

tom 94

nr 3

Histological study on embryogenic culture of the oak [Quercus robur L.]

67%

Ostrolucka M. G., Sunderlikova V., Tsvetkov I. N.

Acta Biologica Cracoviensia. Series Botanica. Supplement

1999

tom 41

nr 1

Slow vacuolar channels of non-embryogenic and embryogenic cultures of winter wheat

67%

Dziubinska H., Filek M., Szechynska-Hebda M., Trebacz K.

Acta Physiologiae Plantarum

2003

tom 25

nr 2

Ograniczanie wyników

2 Acta Physiologiae Plantarum

1 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Dziubinska H.

1 Filek M.

1 Kurczynska E. U.

1 Oleszczuk S.

1 Ostrolucka M. G.

1 Potocka I.

1 Sala K.

1 Sowa S.

1 Sunderlikova V.

1 Szechynska-Hebda M.

1 Trebacz K.

1 Tsvetkov I. N.

1 Zimny J.

1 2013

1 2005

1 2003

1 1999

Wyniki wyszukiwania

A simple and efficient method for cryopreservation of embryogenic triticale calli

Apoplastic markers of cell differentiation status during somatic embryogenesis in Arabidopsis thaliana

Histological study on embryogenic culture of the oak [Quercus robur L.]

Slow vacuolar channels of non-embryogenic and embryogenic cultures of winter wheat