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1

EF-1alpha is a target site for inhibitory effect of quercetin in the peptide elongation process

100%
Marcinkiewicz C., Galasinski W., Gindzienski A.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 3
The effect of quercetin (3,3',4',5,7-pentahydroxyflavone) on the polypeptide elon­gation system isolated from rat liver cells, was investigated. Quercetin inhibited [ 4C]leucine incorporation into proteins in vitro and the inhibitory effect is being directed towards the elongation factor eEF-1, but not to eEF-2 and ribosomes. Quer­cetin was found to form a complex with EF-la, which was inactive in GTP-dependent binding to ribosomes. It can be suggested that quercetin can block the total or the part of the domain of EF-la structure that is responsible for formation of the ternary complex EF-la-GTP-i14C]Phe-tRNA and therefore preventing formation of the quater­nary complex with ribosomes.
2

Isolation and properties of the subunit form EF-1C of elongation factor 1 from Guerin epithelioma cells

100%
Marcinkiewicz C., Galasinski W.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 2
EF-1C is a component of the aggregate EF-1B, consisting of the subunit forms EF-1A-EF-1C; it was isolated by dissociation of this aggregate in the presence of GTF. The subunit form EF-1C stimulates binding of aminoacyl-tRNA to ribosomes, catalysed by EF-1A, similarly as EF-lßy which stimulates the activity of EF-1 in other eukaryotic cells. EF-1C in the presence of 6 M urea was separated into two polypeptides. Polypeptide of molecular mass 32000 Da is responsible for regeneration of the EF-1A-GTP active complex. Thermal sensitivity of EF-1A was much higher than that of EF-1B, thus a protective role of EF-1C in the EF-1AEF-1C complex is suggested.
3

New experimental and computational approaches to the analysis of gene expression

88%
Rafalski J. A., Hanafey M., Miao G. H., Ching A., Lee J. M., Dolan M., Tingey S.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 4
Public and private EST (Expressed Sequence Tag) programs provide access to a large number of ESTs from a number of plant species, including Arabidopsis, corn, soybean, rice, wheat. In addition to the homology of each EST to genes in GenBank, information about homology to all other ESTs in the data base can be obtained. To estimate expression levels of genes represented in the DuPont EST data base we count the number of times each gene has been seen in different cDNA libraries, from different tissues, developmental stages or induction conditions. This quantitation of message levels is quite accurate for highly expressed messages and, unlike conventional Northern blots, allows comparison of expression levels between different genes. Lists of most highly expresses genes in different libraries can be compiled. Also, if EST data is available for cDNA libraries derived from different developmental stages, gene expression profiles across development can be assembled. We present an example of such a profile for soybean seed development. Gene expression data obtained from Electronic Northern analysis can be confirmed and extended beyond the realm of highly expressed genes by using high density DNA arrays. The ESTs identified as interesting can be arrayed on nylon or glass and probed with total labeled cDNA first strand from the tissue of interest. Two-color fluorescent labeling allows accurate mRNA ratio measurements. We are currently using the DNA array technology to study chemical induction of gene expression and the biosynthesis of oil, carbohydrate and protein in developing seeds. In the future, high density DNA arrays containing the whole gene complement of an organism will become available for the analysis of genome-wide patterns of gene expression.
4

Differences in the structures of the elongation factors (EF-2) isolated from guerin epithelioma and rat liver

75%
Sredzinska K., Gajko A., Galasinski W.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1991
|
tom 39
|
nr 2
5

Elongation factors from the Guerin epithelioma and rat liver cells

75%
Galasinski W., Marcinkiewicz C., Gajko A., Sredzinska K., Telejko E.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 4
6

The phosphorylation of elongation factor EF-1 isolated from guerin epithelioma

75%
Marcinkiewicz C., Gajko A., Galasinski W.
Acta Biochimica Polonica
|
1992
|
tom 39
|
nr 1
7

Possible evolution of factors involved in protein biosynthesis

63%
Nyborg J.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 4
The elongation factors of protein biosynthesis are well preserved through out evolution. They catalyze the elongation phase of protein biosynthesis, where on the ribosome amino acids are added one at a time to a growing peptide according to the genetic information transcribed into mRNA. Elongation factor Tu (EF-Tu) provides the binding of aminoacylated tRNA to the ribosome and protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. Elongation factor G (EF-G) supports the translocation of tRNAs and of mRNA on the ribosome so that a new codon can be exposed for decoding. Both these factors are GTP binding proteins, and as such exist in an active form with GTP and an inactive form with GDP bound to the nucleotide binding domain. Elongation factor Ts (EF-Ts) will catalyze the exchange of nucleotide on EF-Tu. This review describes structural work on EF-Tu performed in our laboratory over the last eight years. The structural results provide a rather complete picture of the major structural forms of EF-Tu, including the so called ternary complex of aa-tRNA:EF-Tu:GTP. The structural comparison of this ternary complex with the structure of EF-G:GDP displays an unexpected macromolecular mimicry, where three domains of EF-G mimick the shape of the tRNA in the ternary complex. This observation has initiated much speculation on the evolution of all factors involved in protein synthesis, as well as on the details of the ribosomal function in one part of elongation.
8

Evidence for plant ribosomal 5S RNA involvement in elongation of polypeptide chain biosynthesis

63%
Shaikhin S., Sobkiewicz A., Barciszewski J., Twardowski T.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 1
A series of short oligo-DNA probes (8-10-mers) complementary to various regions of the plant ribosomal 5S ribonucleic acid (5S rRNA) have been synthesized. The results of their hybridization to free 5S rRNA and to ribosomes pointed to the availability of nucleotides in loop "C" for complexation. We found a correlation between hybridization of selected oligonucleotides and their inhibitory effect on enzymatic binding of Phe-tRNA and poly(Phe) synthesis on wheat germ 80S ribosomes. Evidence was obtained for involvement of 5S rRNA in the elongation of polypeptide chain during protein biosynthesis. 5S rRNA seems to play a critical role in protein biosynthesis, probably through causing conformational changes of loop C.
9

Purification and characterization of the protein kinase eEF-2 isolated from rat liver cells

63%
Gajko A., Galasinski W., Gindzienski A.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 4
The elongation factor 2 (eEF-2) protein kinase was isolated from rat liver cells, purified and partly characterized. It was found that the enzyme exists in an inactive form in the homogenate of rat liver. The active fraction of kinase eEF-2 was obtained after removal of the inhibitory substance by hydroxyapatite column chromatography. The purified enzyme is an electTophoretically homogeneous protein with relative molecular mass of approximately 90000 and isoelectric point, pi = 5.9. The enzyme specifically phosphorylates the elongation factor eEF-2 in the presence of calmodulin and Ca2+.
10

Zaleznosc miedzy masa bulw odmian jadalnych a ich wymiarami i ksztaltem

51%
Zarzynska K.
Biuletyn Instytutu Ziemniaka
|
1993
|
nr 43
57-63
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