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1

Efficiency of chicken blastoderm cell transfection combining two nonviral methods: electroporation and synthetic carriers

100%
Kinal A., Kozlowska I., Lakota P., Wawrzynska M., Kijewska E.
Folia Biologica
|
2017
|
tom 65
|
nr 1
2

Cholesterol-induced variations in fluctuations of the pores in bilayer lipid membrane

100%
Koronkiewicz S., Bryl K.
Cellular and Molecular Biology Letters
|
1999
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tom 04
|
nr 4
The constant-current chronopotentiometric measurements of egg yolk phosphatidylcholine bilayer membrane without and with cholesterol are presented. It was demonstrated that the constant-intensity current flow through the bilayer membranes without and with cholesterol generated the oscillating pores in their structures. The presence of cholesterol in the bilayer membrane increased the value of critical potential at which pores could be formed. The shift in the distribution of calculated pore radii towards smaller values was observed in the bilayer containing cholesterol. It was postulated that greater stability of bilayer with cholesterol resulted from increased critical pore radius (at which the bilayer would rupture). The implications of the membrane cholesterol for the application of constant-current method as a biotechnological tool for incorporating molecules of different size are discussed.
3
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Transient expression assay for optimization of direct gene transfer into cucumber meristem protoplasts by electroporation

100%
Burza W., Wochniak P., Wroblewski T., Malepszy S.
Journal of Applied Genetics
|
1995
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tom 36
|
nr 1
The paper presents a new way of obtaining viable and very homogeneous cucumber protoplasts. Protoplasts from cells formed in the shoot tip meristem culture were isolated from suspension. Plasmid pBI121 was introduced using impulse electric field. Effectiveness of transformation process was determined by the transient expression of ß-glucuronidase (GUS) gene, controlled by promotor 35S. The activity of ß-glucuronidase enzyme as a product of GUS reporter gene was estimated by fluorimetric method (JEFFERSON 1987). Parameters of electroporation process were optimized. The transient expression of GUS gene was measured 24 h after electroporation. The highest effectiveness of transformation process was achieved using three electric impulses at the initial voltage of 250-350 V at 10-sec. intervals as a result of discharging a 140 µF capacitor and 50-70 µg × cm⁻³ plasmid DNA in the presence of 50 µg × cm⁻³ carrier DNA. The system presented is an effective method of exogenic DNA transfer, which is indicated by a high transient expression of the reporter gene. In comparison to Agrobacterium tumefaciens and A. rhizogenes, this alternative method of gene transfer can be used for obtaining transgenic cucumber plants.
4

Electroporation of Thiobacillus versutus with plasmid DNA

100%
Wlodarczyk M., Jagusztyn-Krynicka E. K., Bartosik D., Kalinowska I.
Acta Microbiologica Polonica
|
1994
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tom 43
|
nr 2
5

Messenger RNA electroporation: an efficient tool in immunotherapy and stem cell research

100%
Van Driessche A., Ponsaerts P., Van Bockstaele D. R., Van Tendeloo V. F. I., Berneman Z. N.
Folia Histochemica et Cytobiologica
|
2005
|
tom 43
|
nr 4
6

On stability of circular hole in membrane bilayer

100%
Fosnaric M., Kralj-Iglic V., Hagerstrand H., Iglic A.
Cellular and Molecular Biology Letters
|
2001
|
tom 06
|
nr 2
7

Membrane electroporation: EPR estimation of pore resealing time

100%
Salwinski L., Froncisz W.
Current Topics in Biophysics
|
1994
|
tom 18
|
nr 1
8

Reversible and irreversible electroporation of cell suspensions flowing through a localized dc electric field

84%
Korohoda W., Grys M., Madeja Z.
Cellular and Molecular Biology Letters
|
2013
|
tom 18
|
nr 1
Experiments on reversible and irreversible cell electroporation were carried out with an experimental setup based on a standard apparatus for horizontal electrophoresis, a syringe pump with regulated cell suspension flow velocity and a dcEF power supply. Cells in suspension flowing through an orifice in a barrier inserted into the electrophoresis apparatus were exposed to defined localized dcEFs in the range of 0–1000 V/cm for a selected duration in the range 10–1000 ms. This method permitted the determination of the viability of irreversibly electroperforated cells. It also showed that the uptake by reversibly electroperforated cells of fluorescent dyes (calcein, carboxyfluorescein, Alexa Fluor 488 Phalloidin), which otherwise do not penetrate cell membranes, was dependent upon the dcEF strength and duration in any given single electrical field exposure. The method yields reproducible results, makes it easy to load large volumes of cell suspensions with membrane non-penetrating substances, and permits the elimination of irreversibly electroporated cells of diameter greater than desired. The results concur with and elaborate on those in earlier reports on cell electroporation in commercially available electroporators. They proved once more that the observed cell perforation does not depend upon the thermal effects of the electric current upon cells. In addition, the method eliminates many of the limitations of commercial electroporators and disposable electroporation chambers. It permits the optimization of conditions in which reversible and irreversible electroporation are separated. Over 90% of reversibly electroporated cells remain viable after one short (less than 400 ms) exposure to the localized dcEF. Experiments were conducted with the AT-2 cancer prostate cell line, human skin fibroblasts and human red blood cells, but they could be run with suspensions of any cell type. It is postulated that the described method could be useful for many purposes in biotechnology and biomedicine and could help optimize conditions for in vivo use of both reversible and irreversible electroporation.
9

In vitro evaluation of electroporated gold nanoparticles and extremely-low frequency electromagnetic field anticancer activity against Hep-2 laryngeal cancer cells

84%
Alshehri M. A., Wierzbicki P. M., Kaboo H. F., Nasr M. S. M., Amer M. E., Abuamara T. M. M., Badr D. A., Saleh K. A., Fazary A. E., Mohamed A. F.
Folia Histochemica et Cytobiologica
|
2019
|
tom 57
|
nr 4
10

Influence of electroporation on chicken blastoderm cell viability in vitro

84%
Wawrzynska M., Bednarczyk M., Lakota P., Lubiszewska M.
Folia Biologica
|
2008
|
tom 56
|
nr 3-4
197-201
11
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Electroporation and morphogenic potential of Gentiana kurroo (Royle) embryogenic cell suspension protoplasts

84%
Wojcik A., Rybczynski J. J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
12

Alternative methods of plant transformation - a short review

84%
Rakoczy-Trojanowska M.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr 3
Several methods of transformation are currently available for delivering exogenous DNA to plant cells. Agrobacterium-mediated transformation, microprojectile bombardment and direct protoplast transformation are routinely used today. However, each of them has certain disadvantages, which led to research into the development of novel alternative systems such as infiltration, electroporation of cells and tissues, electrophoresis of embryos, microinjection, pollen-tube pathway, silicon carbide- and liposome-mediated transformation. The low efficiency of transformation is considered to be the main reason for the limited popularity of the alternative transformation methods, other than infiltration and silicon carbide-mediated transformation, which seem to be the most promising ones for practice.
13

The loading of human erythrocytes with small molecules by electroporation

84%
Saulis G.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr 1
14

Nonviral transfection of human umbilical cord blood dendritic cells is feasible, but the yield of dentritic cells with transgene expression limits the application of this method in cancer immunotherapy

84%
Markowicz S., Niedzielska J., Kruszewski M., Oldak T., Gajkowska A., Machaj E. K., Skurzak H., Pojda Z.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr 1
Dendritic cells (DC) generated from human umbilical cord blood might replace patients' DC in attempts to elicit tumor-specific immune response in cancer patients. We studied the efficiency of transfection of human cord blood DC with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene, to test if nonviral gene transfer would be a method to load DC with protein antigens for immunotherapy purposes. Cord blood mononuclear cells were cultured in serum-free medium in the presence of granulocyte-monocyte colony stimulating factor (GM-CSF), stem cell factor (SCF) and Flt-3 ligand (FL), to generate DC from their precursors, and thereafter transfected by electroporation. Maturation of DC was induced by stimulation with GM-CSF, SCF, FL and phorbol myristate acetate (PMA). Transfected DC strongly expressed EGFP, but transfection efficiency of DC, defined as HLA-DR+ cells lacking lineage-specific markers, did not exceed 2.5%. Expression of the reporter gene was also demonstrated in the DC generated from transfected, purified CD34+ cord blood cells, by stimulation with GM-CSF, SCF, FL, and tumor necrosis factor α (TNF-α). Transfection of CD34+ cells was very efficient, but proliferation of the transfected cells was much reduced as compared to the untransfected cells. Therefore, the yield of transgene-expressing DC was relatively low. In conclusion, nonviral transfection of cord blood DC proved feasible, but considering the requirements for immunotherapy in cancer patients, transfection of differentiated DC or generation of DC from transfected hematopoietic stem cells provide only a limited number of DC expressing the transgene.
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