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Seedlings and young plants of lenten rose (Helleborus orientalis Lam.) were grown for two years (2006–2007) in containers on peat substrate which was deacidified with calcium carbonate at doses (g∙dm⁻³) of 2.5, 5.0, 7.5 and 10.0. Within each dose of CaCO3 plants were divided into two groups, of which one was fertilized with a lower (0.1%) and the other with a higher (0.3%) concentration of Peters Professional PL Special (15:11:29). The CaCO3 dose applied to deacidify high moor peat had a significant effect on plant height irrespective of their age. In the first and second year of pot cultivation the tallest plants were produced on a substrate to which calcium carbonate was added at 2.5 g·dm⁻³ CaCO3, while the lowest at 10 g·dm⁻³ CaCO3. The number of leaves and flowering of lenten rose did not depend on the amount of calcium carbonate introduced to the substrate. Fertilization of lenten rose cv. ‘New Hybrids F1’ with Peters Professional PL Special (15:11:29) at a higher concentration (0.3%) made it possible to produce taller plants, with a larger number of leaves, and earlier and more abundant flowering.
The new antibacterial collagen dressing implant was constructed. The dressing was composed of collagen sponge saturated with liposomal polymyxin B (Lip-PolB). As a control an alternative dressing composed of collagen sponge saturated with free polymyxin B (Free-PolB) was used. The release of the antibiotic from the Lip-PolB was measured by diffusion of the drug into polyurethane sponge saturated with sterile broth employing “polyurethane sponge model” published previously. The antibiotic was released from Lip-PolB four times slower than from Free-PolB. Antibacterial activity of the dressing was tested using also polyurethane sponge model, with polyurethane sponge saturated with the bacterial broth culture of P. aeruginosa (108 CFU/ml). After 1, 2 and 3 days of incubation at 37°C, polyurethane sponges were sonicated in the tubes with PBS; then the CFU number was examined by plating. The Lip-PolB proved again to be several times less effective than Free-PolB. We can draw a conclusion that collagen sponge with liposomal polymyxin B can serve as an effective reservoir of the drug for control of infection in superficial wounds. However, the concentration of liposomal drug applied topically should be several times higher than the concentration of free drug.
Following the limitations regarding the use of the neonicotinoids: clothianidin, thiamethoxam and imidacloprid there are no currently available insecticide seed dressings for oilseed rape in Poland. For maize here is only one seed dressing containing methiocarb available with a very narrow registered scope of use. The impact of limitations on protection possibilities of other major Polish agricultural crops is either negligible or non-existent. In consequence a group of economically important insect pests of maize [dungbeetles (Melolonthidae); click beetles (Elateridae); noctuid moths (Agrotinae)] and oilseed rape [leaf miners (Agromyzidae), turnip sawfly (Athalia colibri Christ.), cabbage weevils (Curculionidae), cabbage root fly (Hylemyia brassicae Bche.), diamond-back moth (Plutella maculipennis Curt.)] is left without any legal possibility of chemical control. For the other important pests of the early growth stage of oilseed rape development, there are only pyrethroids available together with one product containing chloropiryfos that can be applied once per vegetation season. Since both maize and oilseed rape are grown in Poland on the area of approximately 1 million ha (each crop), this situation raises concerns about production possibilities as well as development of pest resistance.
Background. Salad dressings due to their gustatory and nutritious advantages have been very often used in many food products. Traditional dressings usually contain large amount of fat (20-65%). Reduction of its content in such Systems in most cases leads to their physical destabilization and causes worsening of sensory properties particularly because of increase in volatility of lipophilic aroma compounds which have been mostly found in food products. The objective of this study was to investigate the influence of hydrocolloid type and concentration on the retention and release profile of diacetyl and (-)-α-pinene in model reduced fat salad dressings formed with sodium caseinate. Material and methods. Model reduced fat salad dressings containing 10 wt% vegetable fat and stabilized by both 1 wt% sodium caseinate and 0.01-0.2 wt% hydrocolloids (xan- than gum, guar gum, gum arabie, native pregelatinised rice starch - Remyline XS-DR-P and acetylated distarch phosphate - Microlys CA - produced from potato starch) were investigated. The samples were flavoured with diacetyl or (-)-α-pinene at concentration of 0.1 wt% for each flavour compound. Following measurements were done: dressings characteristics (oil droplet size and distribution, apparent viscosity, thermodynamic and kinetic stability), stability of the odorants (relative retention and release profile) and odour intensity by method using scale. Results. The studies showed significant dependence (a < 0.001) between relative retention of the investigated odorants and hydrocolloid type as well as concentration. Moreover, it was detected that the values of persistence index of flavour compounds (related to the release profile) were in majority significantly correlated (a < 0.05) with viscosity and creaming stability of the dressings, whereas relatively low and mostly statistically insignificantly with mean droplet size (D[3,2]). Furthermore, significant correlation values (a < 0.05) were registered between odour intensity and vapour/liquid partition coefficient of the aroma compounds (K) in salad dressings containing: xanthan gum, guar gum, and gum arabie. Conclusions. Xanthan gum and guar gum markedly improved stability of hydrophilic diacetyl and hydrophobic (-)-α-pinene in dressings containing 10 wt% rapeseed oil, whereas rice starch - Remyline XS-DR-P and acetylated distarch phosphate-Microlys CA in- creased significantly retention of hydrophilic aroma compound.
The studies conducted in a growth chamber observed the protective effect of biopreparations such as Biosept 33 SL, Biochikol 020 PC and Polyversum, used as seed dressing for common bean and pea. The fungicide Zaprawa Oxafun T was used as a comparison, while the seeds that were not dressed constituted the control. The studied plants grew in the medium grown over with different species of fungi pathogenic towards pea and common bean. Irrespective of the species, the fewest plants, with the greatest proportion of infected ones, were obtained in control combinations. The application of biopreparations and the fungicide turned out to be an effective protection of pea and common bean from the infection by the examined phytopathogens. Biosept 33 SL in the concentration of 0.2% showed to have the best effect among the tested biopreparations.
W 2008 r. kontynuowano badania polowe rozpoczęte w 2006 r., podczas których określano skuteczność zaprawiania mieczyka wybranymi preparatami na ograniczenie porażenia bulw potomnych przez patogeny grzybowe Fusarium oxysporum. Mniej licznie izolowano grzyby z rodzaju Alternaria, Cylindrocarpon oraz Penicillium. W latach 2006-2008 podczas badań polowych określano skuteczność zaprawiania mieczyka wybranymi preparatami na ograniczenie porażenia bulw potomnych przez patogeny grzybowe. W badaniach zastosowano do zaprawiania wiosennego bulw następujące preparaty: Biochikol 020 PC, Biosept 33 SL, Polyversum oraz jako kombinacje porównawcze Sumilex 500 SC oraz mieszaninę Topsinu M 500 SC z Kaptanem zaw. 50 WP. Do badań użyto trzy odmiany mieczyka: ʻOscar’, ʻPriscilla’ i ʻSancerre’. Kontrolę stanowiły bulwy niezaprawiane. Uzyskane wyniki wykazały, że najskuteczniejszym zabiegiem, istotnie polepszającym zdrowotność i jakość bulw mieczyka wszystkich badanych odmian było zastosowanie mieszaniny preparatów Topsin M 500 SC i Kaptan zaw. 50 WP. Sumilex 500 SC oraz preparaty biotechniczne Biochikol 020 PC i Biosept 33 SL korzystnie wpływały na stan zdrowotny bulw mieczyka w porównaniu z kontrolą. Najmniej skuteczne było zastosowanie preparatu biologicznego Polyversum WP. Najmniejszą ilość kolonii grzybów wyizolowano z bulw potomnych z kombinacji Topsin M 500 SC+Kaptan zaw. 50 WP, zwłaszcza na odmianie ʻSancerre’ oraz z bulw odmiany ʻOscar’ zaprawionych preparatem Sumilex 500 SC, a najliczniej z obiektów kontrolnych wszystkich badanych odmian. Z porażonych bulw najliczniej izolowano grzyby z rodzaju Fusarium, a zwłaszcza gatunek Fusarium oxysporum. Mniej licznie izolowano grzyby z rodzaju Alternaria, Cylindrocarpon oraz Penicillium.
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