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The structure and morphometry of the epididymis in the greater cane rat were studied in this work. In assessing the morphology and characterising the morphometric values, a total of 15 adult male greater cane rats, bred and raised in captivity, were used. All the animals had brownish perineal staining, which was taken as index of sexual maturity in male cane rats, and they were maintained on elephant grass stems with water given ad libitum. From this work, the epididymis of the greater cane rat was observed to have a mean weight of 0.0365 ± 0.091 g, forming about 0.016% of the total body weight and an average volume of 0.36 ± 0.08 mL. There was a positive correlation between the epididymal weights, testicular weight, and the body weight in this animal. However, the gross divisions of the epididymis into head, body, and tail were not conspicuous in the cane rat; instead it had two divisions — the cranial and the caudal divisions. In addition, based on the histological and histomorphometric analyses, five zones were observed in the epididymal epithelium of this animal. This preliminary information on the epididymis will serve as a basis for further research on the epididymis of the greater cane rat and will contribute to the knowledge of the its reproductive biology, which will subsequently aid in the captive rearing and domestication of this animal. (Folia Morphol 2010; 69, 4: 246–252)
Morphometrical and biochemical-genetic comparisons were performed between wild IMustela vison energúmenos Bangs, 1896) and ranch mink (Dark Standard strain) to investigate intraspecific differences and to characterize effects of the domestication in this species. All animals were kept under similar conditions in larger open air enclosures prior to dissection to keep modificatory influences on the measures low and comparable. In the morphometrical part of this study weights of the total body, brain, eyes, thoracal viscera, heart, abdominal viscera, liver, spleen, kidneys, adrenals, and pancreas of 82 (39 males, 43 females) wild and 97 (50 males, 47 females) ranch mink were compared using the allometrical method with the net carcas weight as the reference parameter. Only three organs were significantly smaller in size in the ranch mink group: brain, heart, and spleen. Size decreases may result from reductions of central nervous and circulatory functions in the domesticated organism. They were compared with results in other species and evaluated as a genetically linked intraspecific adaptation to the special ecological demands of domestication. Twenty five proteins encoded by products of 44 genetic loci were compared electrophoretically between 7 wild and 7 ranch mink. Except for one esterase isozyme locus all genes examined were monomorphic. The protein heterozygosity was rather low in both groups. These results were discussed in connection with certain bottleneck situations, with investigations in other species, and with the short domestication time of ranch mink.
The inheritance of the dehiscent pod character was investigated in two recombinant inbred populations using a simplified correlation analysis. The approach identified three regions on the pea genome that affect the expression of pod dehiscence. The region on linkage group III corresponded to the expected position of Dpo, a gene known to influence pod dehiscence. A locus on linkage group V appeared to have a slightly smaller effect on expression of the phenotype. The third region was observed only in one cross, had a greater effect than Dpo, and was postulated to be yellow pod allele at the Gp locus.
Silybum marianum (milk thistle) is among the top selling herbal products in the U.S., in Italy and in other countries. Despite its economic importance as an officinal and medicinal species, only few improved genotypes exist and the species is still marked by traits that are typical of non-domesticated plants. Based on this evidence, further genetic improvement is required for the complete exploitation of milk thistle as a crop species. The first step in order to allow further breeding programmes of the species is the setup of a reliable crossing protocol. The present paper, after a brief review of S. marianum flower anatomy, describes a crossing method for this plant. The correct phenological stage for emasculation is BBCH growth stage 54. If manual pollination is repeated two days after emasculation, a significant increase of F1 fruits can be obtained. By utilizing the proposed protocol, an average 70–90 florets per flower head are emasculated and percentage of success (F1 fruits harvested) usually ranges between 27 and 70%. The proposed crossing protocol will constitute an important tool in order to allow the further genetic improvement of this species.
The wild-harvested fruit of Vaccinium oxycoccos (European cranberry) is used medicinally in many European and North American countries; the plant, however, is seldom cultivated. In order to optimize the collection strategy and improve the horticulturally important characters of V. oxycoccos clones, comprehensive investigations of the species are necessary. In the present study we investigated the phenological, morphological and genetic diversity of 29 clones originating from two wild populations growing in two strictly protected Lithuanian reserves, Čepkeliai and Žuvintas. During an ex situ collection at Kaunas Botanical Garden, we observed great phenological variation between the collected V. oxycoccos clones. The following morphological traits most clearly distinguished our study clones: leaf size, berry shape, berry size and fruit colour at full maturity. The genetic variation of V. oxycoccos clones from the two populations was assessed using RAPD and SSR. RAPD analysis conducted with 9 primers resulted in 146 polymorphic loci for the total sample, and SSR analysis with 5 primers revealed 29 alleles for the total sample. A greater degree of polymorphism was demonstrated for the Čepkeliai population than for the Žuvintas population. The study allowed the selection of several clones having promising morphological traits for further testing in the field.
The study was carried out to investigate the domestication potential of Flacourtia jangomas (Lour.) Raeusch, a wild fruit species in Bangladesh, through nursery raising from seeds and clonal propagation by stem cutting. Air dried seeds were treated with four different pre-sowing treatments i.e., control (T0), seeds soakedin coldwater for 24 h (T1), 48 h (T2), or 72 h (T3) to explore the seedgermination ability of the species. Pre-sowing treatments significantly enhancedthe germination period, germination percentage andbiomass production of seedlings. The early germination (least imbibition period), highest germination percentage (81.3) and total dry biomass (0.52 g) was observed in T2 (seeds soaked in cold water for 48 h) while the lowest germination percentage (53.7) andtotal dry biomass (0.23 g) was observedin T3 andT0 respectively. The plant species was highly amenable for rooting for clonal propagation. However, the rooting ability of cuttings was significantly affectedby the application of IBA. The highest rooting percentage (100), maximum root number (5.63), the longest root length (3.28 cm) andbest survival (85.0%) were obtainedfrom the cuttings treatedwith 0.4% IBA solution followed by 0.2% IBA andthe lowest was in cuttings without treatment. Therefore, pre-sowing treatment ‘soaking of seeds in cold water for 48 h for nursery raising and ‘0.4% IBA treatment’ of stem cuttings for clonal propagation may be recommended for mass production of quality planting stocks for the domestication of the species through homestead agroforestry or in fruit orchards.
Based on protein polymorphism and results obtained with RAPD-PCR and ISSR-PCR methods, the domestic and wild Artiodactyla and Perissodactyla (14 and 7 species, respectively) were compared. The marker-specific species differentiation in domestic and wild species was observed, leading to the hypothesis of the “subgenome” existing in domestic species. It is assumed that “subgenome” contains certain genes encoding important proteins and enzymes. In the past, the high variation of “subgenome” could play an essential role in domestication, leading to the wide morphological differentiation of contemporary domestic species.
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