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Biomass accumulation and diterpenoids biosynthesis in Salvia austriaca transformed roots grown in a bioreactor

100%

Kuzma L., Klos K., Wysokinska H.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2015

tom 96

nr 1

The effect of methyl jasmonate on production of antioxidant compounds in shoot cultures of Salvia officinalis L.

100%

Grzegorczyk I., Wysokinska H.

Herba Polonica

2009

tom 55

nr 3

238-243

The shoots of Salvia officinalis growing in MS liquid medium supplemented with IAA 0.1 mg l-1) and BAP (0.45 mg l-1) were treated with methyl jasmonate (MeJA) to increase production of compounds with antioxidant activity (carnosic acid, carnosol and rosmarinic acid). The increase in metabolite production depended on MeJA concentration, the period of exposure to elicitor and type of compound. The MeJA action was observed 24 h after elicitation. It was found that the maximum level of diterpenoids, calculated as the sum of CA and Car (about 8 mg g-1 dry wt) was achieved at 3 days after elicitation with 20 µM methyl jasmonate. The highest amount of rosmarinic acid (about 41 mg g-1 dry wt) was achieved with 50 or 100 μM methyl jasmonate on the 5th day after elicitation. It was almost 2-fold higher compared to the control (cultures treated with only ethanol).

Ograniczanie wyników

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Herba Polonica

2 Wysokinska H.

1 Grzegorczyk I.

1 Klos K.

1 Kuzma L.

1 2015

1 2009

Wyniki wyszukiwania

Biomass accumulation and diterpenoids biosynthesis in Salvia austriaca transformed roots grown in a bioreactor

The effect of methyl jasmonate on production of antioxidant compounds in shoot cultures of Salvia officinalis L.