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Background. The aim of this study was to evaluate the dynamics of changes in IgG anti-C6VlsE concentration in patients treated for Lyme borreliosis in relation to clinical manifestations of the disease. Material and methods. The study group consisted of 20 patients from Ternopil and surrounding areas (Western Ukraine) with clinical symptoms of Lyme borreliosis. IgG anti-C6VlsE was measured three times: before starting the patients on antibiotic therapy, immediately after its completion and 3-4 months after the end of antibiotic therapy. Results. In 40% of the patients the IgG anti-C6VlsE concentration decreased 3-4 months after the treatment, but only in half of them it was associated with elimination of symptoms. 60% of patients did not show any tendency to decrease in IgG anti-C6VlsE concentrations after 3-4 months after the treatment, and 25% of patients in this group had an improvement in health condition. Conclusions. It cannot be excluded that IgG anti-C6VlsE may be important in the serological evaluation of the efficacy of Lyme borreliosis treatment, especially in early stage patients. However, this requires further research, which should be extended to a larger group of patients. It is also important that the assessment of anti-C6VlsE antibody concentration should be performed additionally for a period longer than 4 months from the end of antibiotic therapy.
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A short review on proteomics and its applications

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Proteomics is the large scale of study of proteins, particularly their function and structure. Proteomics is an excellent approach for studying changes in metabolism in response to different stress conditions. In the present review focused on different types of techniques for the analysis of expressed proteins. The techniques includes 2-D gel electrophoresis, MALDI-TOF/MS etc., play a vital role for the analysis of novel proteins and their role in disease maintenance and treatment. The review also concentrated on applicative perspective of proteomics in the fields of biomedical, agriculture and food.
The aim of the studies was the evaluation of relationship between the tetracycline residue levels in milk of cows treated intrauterinary with different doses of tetracycline and clinical form of endometritis. Six cows were treated by intrauterine route with pessaries containing tetracycline hydrochloride. The cows in group 1 (n = 3) were affected with acute endometritis, and the cows in group 2 (n = 3) by chronic endometritis. The animals were treated with Tetramykoin supp. uter. ad us. vet. (Léčiva, Praha, Czech Republic) at a dosage of 3g/cow (group 1), and 2g/cow (group 2). Milk samples were collected before treatment and at 12-h intervals for 144 h. The samples were analysed by the high-performance liquid chromatography method with solid phase extraction. The mean concentrations of tetracycline in milk 12 h after treatment were 227.7 μg.1⁻¹ in both groups. The tetracycline residues reached their maximum mean concentrations 24 h after the treatment (276.4 μg.1⁻¹, and 369.6 μg.1⁻¹, for group 1 and 2 resp.). Residues of 13.7 μg.1⁻¹ were detected in group 2 at 108 h. At 120 h the mean concentrations were 33.1μ g.1⁻¹ for group 1 and zero for group 2. No residues were detected after 144 h.
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