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Search for variability among mastreviruses showing symptoms of maize streak virus from different regions of Nigeria by polymerase chain reactions

100%

Oluwafemi S., Thottappilly G., Alegbejo M. D.

Journal of Plant Protection Research

2008

tom 48

nr 4

529-539

Field surveys were undertaken in 1997-1999 across five ecological zones in Nigeria to collect grass species showing symptoms of Maize streak virus (MSV) (Genus Mastrevirus: Family Geminiviridae). Apart from maize (Zea mays L.), 15 grass species were found with symptoms similar to MSV. These hosts showed two types of symptoms viz: mild or severe. Polymerase chain reaction (PCR) and immunocapture IC-PCR were used to diagnose the mastreviruse. Variation among the isolates is illustrated in relationship dendograms. The PCR dendogram showed 35-85% variation among the isolates while the IC-PCR dendogram showed 55-85%. The PCR dendogram clusters the isolates essentially along symptom severity. Isolates that were most distantly related to a standard MSV strain showed mild symptoms in their grass hosts. Simple PCR protocols could help in studying diversity among grass Mastreviruses where facilities for genome sequencing are not available.

In vitro effectiveness of selected acaricides against red poultry mites (Dermanyssus gallinae, De Geer, 1778) isolated from laying hen battery cage farms localised in different regions of Poland

86%

Zdybel J., Karamon J., Cencek T.

Bulletin of the Veterinary Institute in Puławy

2011

tom 55

nr 3

Investigation was carried out on red poultry mites Dermanyssus gallinae collected from 32 batten- cage farms of laying hens localised in 14 voivodships. The following insecticides were used: Alfasekt (α-cypermetrin), Blaxime (phoxim), ByeMite (phoxim), Ficam (bendiokarb), Galtox B (fenitrotion, dichlorphos, α-cypermetrin), Knox-out (diazinon - microcapsule form), Master (chlorpyrifos - microcapsule form), Skorpion (sevin, α-cypermetrin), Sumition (fenitrotion - microcapsule form). The efficacy of the acaricides was investigated on the plates using the veneer disc as the ground. The insecticides were used in concentrations and volumes recommended by producers of preparations. Efficacy study showed that the most effective against most of red mite populations were phoxim Blaxime and Bye Mite (89%-100% in 13 voivodships) and one carbamate preparation - Ficam (92%-100% in 11 voivodships). Relatively effective turned out to be also another carbamate acaricide with α-cypermetrin supplement - Skorpion. Phosphoroorganics in microcapsule form and acaricides containing only α-cypermetrin demonstrated low effectiveness.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Journal of Plant Protection Research

1 Alegbejo M. D.

1 Cencek T.

1 Karamon J.

1 Oluwafemi S.

1 Thottappilly G.

1 Zdybel J.

1 2011

1 2008

Wyniki wyszukiwania

Search for variability among mastreviruses showing symptoms of maize streak virus from different regions of Nigeria by polymerase chain reactions

In vitro effectiveness of selected acaricides against red poultry mites (Dermanyssus gallinae, De Geer, 1778) isolated from laying hen battery cage farms localised in different regions of Poland