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Desiccation-tolerant plants can be grouped into two categories: the 1) desiccation-tolerant plants whose internal water content rapidly equilibrates to the water potential of the environment and 2) the modified desiccation-tolerant plants that all employ mechanisms to retard and control the rate of water loss. Desiccation tolerance can be achieved by mechanisms that incorporate one of two alternatives, viz. cellular protection or cellular recovery (repair). The majority of plants probably utilize aspects of both. Desiccation-tolerant species, in particular the moss Tortula ruralis, appear to utilize a tolerance strategy that combines a constitutive protection system and a rehydration-inducible recovery mechanism. The rehydration-induced recovery mechanism of Tortula ruralis relies heavily upon a change in gene expression that is mediated by posttranscriptional events rather than the slower reacting transcriptional controls. Findings indicate that it takes a certain amount of prior water loss to fully activate the protein-based portion of the recovery mechanisms upon rehydration. Utilizing cDNAs representing individual hydrins (proteins whose synthesis is hydration specific) and rehydrins (proteins whose synthesis is rehydration specific), it was determined that if drying rates were slow rehydrin transcripts selectively accumulate in the dried gametophytes. Studies revealed that this storage involves the formation of mRNPs (messenger ribonucleoprotein particles). The identity and possible functions of the rehydrins of Tortula ruralis are also under investigation, in particular Tr155, a small rehydrin (24kD) appears to be involved in antioxidant production during rehydration.
The array of genes isolated and characterised from Craterostigma to date allow many insights to be made into the protection mechanisms underlying the desiccation tolerance phenomenon and drought-induced changes in gene expression. However, more work is necessary to define which gene products positively contribute to stress tolerance and which may be considered as secondary stress metabolites or as gene regulators. It is apparent that the gene regulatory mechanisms involved are complex, but future work with more refined biochemical and genetic approaches, together with the analysis of defined mutants will greatly help to dissect the important area of desiccation perception, perhaps faciliate manipulation of desiccation tolerance in a genetic engineering context, and undoubtedly bring much progress in the coming years.
Effects of several stratification variants on seed dormancy breaking were compared in Crataegus submollis Sarg. (hairy cockspur-thorn or Quebec hawthorn). Ripe seeds were collected (in October), cleaned, and dried to a moisture content of 7–12%. Seed dormancy in this species was broken most effectively by warm-cold stratification of nutlets, in a substrate or without any substrate, at 15~25°/3°C or 20~30°/3°C, i.e. with a cyclically alternating warm stage (16+8 hrs or 24+24 hrs/cycle) lasting 16–20 weeks, followed the cold stage lasting ca. 20 weeks, i.e. till the appearance of the first germinating seeds. After stratification, emergence rate is equally high (ca 50%) at cyclically alternating temperatures of 3~15°C and 3~20°C (16+8 hrs). Chemical scarification of nutlets in 96% sulphuric acid for 3 hrs, followed by warm-cold stratification at 20~30°/3°C, with a short, 4-week warm stage, also ensures a high emergence rate (58%). Seed desiccation (in nutlets) slowly to a moisture content of 10–12%, after stratification in a substrate or without any substrate as well as after scarification, results in a reduced emergence rate, especially if seeds are dried to the lower moisture content. Seed storage (in nutlets after drying to a moisture content of 10%) for 10 years at –3°C, does not decrease the emergence rate (93%) after stratification at 20~30°/3°C in a substrate, with a cyclically alternating warm stage (24+24 h) lasting 16 weeks.
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Seed dormancy breaking in Crataegus pedicellata

72%
The effects of stratification and scarification on seed dormancy breaking were compared in scarlet hawthorn (Crataegus pedicellata Sarg. = C. coccinea L). Ripe fruits were collected (in October) and the extracted nutlets were cleaned, and dried to a moisture content of 9–12%. Seed dormancy in this species was broken most effectively by warm-followed-by-cold stratification of nutlets, in a substrate or without any substrate, as well as at 15~25° or 20~30°C, i.e. with a cyclically alternating warm stage (16+8 hrs or 24+24 hrs/cycle) lasting 16–20 weeks, followed by the cold stage at 3°C lasting ca. 20 weeks, i.e. till the appearance of the first germinating seeds. After stratification, emergence rate is equally high (ca 76%) at cyclically alternating temperatures of 3~15°C or 3~20°C (16+8 hrs). Chemical scarification of nutlets in 96% sulphuric acid for 2 hrs, followed by warm-cold stratification at 20~30°/3°C, with a short, 4-weeks warm stage, also ensures a high emergence rate (85–93%). Seed desiccation (in nutlets) slowly to moisture content of 12–14%, after stratification in a substrate or scarification does not reduce the seedling emergence of seeds. Emergence decreased when seeds were desiccated after stratification without any substrate. Results provide new methods of breaking of dormancy and high germination and emergence of hard-coated Crataegus seeds in controlled conditions.
The aim of this study was to explain how temperature, desiccation, light and nitrate affect the seasonal pattern of germination and persistence of dandelion achenes. Directly after collection, Taraxacum officinale Web. ex Wiggers achenes were characterized by non-deep primary dormancy, while dry stored at a low temperature, they showed slight seasonal variations of dormancy level. Both high and low soil temperatures caused dormancy release in dandelion seeds. Dandelion achenes did not form a persistent soil bank. The decline of viable seeds was probably due to long-term sub-zero soil temperatures accompanied by its considerable hydration. Nitrate may be a component of the system of environmental signals which, along with light and desiccation, would enable dandelion seeds to detect even the smallest gaps in dense vegetation. As the dormancy changes are fast, the detection of these gaps by Taraxacum officinale achenes may be especially effective. Such ability of dandelion seeds may explain the remarkable ease with which this species occupies grassland areas densely covered with vegetation.
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Seed dormancy breaking in Crataegus laevigata

58%
Laboratory experiments were made to determine the optimum conditions for dormancy breaking in the midland hawthorn (Crataegus laevigata (Poir.) DC. = C. oxyacantha L.). Its small applelike fruits should be collected when they are fully ripe (in Poland in October). The nutlets extracted from the fruits must be dried at room temperature to the moisture content of 9–13%. The dormancy of midland hawthorn seeds can be overcome by: (1) stratification in a moist medium: 20~30°C/3°C, 16–20 weeks at 20~30°C (16+8 hrs or 24+24 hrs) followed by 16–18 weeks at 3°C, i.e. to the time when first radicles start to appear; or (2) chemical scarification in concentrated sulphuric acid for 2 or 3 hrs, followed by warm stratification at 27.5°C or 20~30°C for 4 weeks and cold stratification at 3°C, lasting 19–21 weeks, i.e. to the time when first radicles start to appear. The stratified seeds germinate vigorously (in 3–5 weeks) and at a high percentage at temperatures of 3~15°C or 3~20°C (16+8 hrs) and all seedlings emerge in such conditions about 4–6 weeks after sowing. Seed germination after stratification or scarification can be stopped by partial desiccation of seeds. Seed desiccation after stratification to the moisture content of 10–13% and sealed storage at –3°C for one year do not reduce seed germination and seedling emergence rates of the previously pretreated seeds. Storage for 20 months at –3°C of seeds dried after harvest to the moisture content of 14% does not reduce their germination and seedling emergence.
Quercus alnifolia is an endemic species in Cyprus, and it is rated as Vulnerable (VU) in the Red List of Oaks. Although Q. alnifolia has a great ecological importance, there are only few studies about this species. In this work we have studied the natural variability of this species by analyzing acorn dimensions, acorn mass, length of embryo, moisture content of acorns and seed germinability among the seven acorn provenances, collected in Cyprus. We also determined the seeds responses to drying. Germination trials were also carried out, and differences in seed germination among populations were examined. Rate of water loss and its effect on seed germination was also estimated by application of specific desiccation treatments. The results showed that acorn characteristics significantly differed between the populations, following a general trend to reduce their dimensions and mass with the altitude increase. Seed germination was high for all studied populations, and germination behaviour was similar for all populations. Desiccation of acorns below 35% resulted in a great reduction of seed germination capacity.
Maturation of Norway maple (Acer platanoides L.) seeds produces deep physiological dormancy and resistance to desiccation. This study used two-dimensional electrophoresis to investigate the protein products of genes activated during the complex developmental process of maturation. Qualitative and quantitative changes in protein composition during maturation were tracked in this species. The most intensive changes in protein content appeared at the end of seed maturation, in embryo axes and cotyledons. During this time their protein content increased significantly and new proteins appeared. Presumably the proteins Q (15 kDa, pI 8) and X (16 kDa, pI 5) separated from cotyledons are associated with maturation of seeds.
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