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Isolation and expression of CsFAD7 and CsFAD8, two genes encoding omega-3 fatty acid desaturase from Camellia sinensis

100%

Ma Q.-P., You E., Wang J., Wang Y., Ding Z.-T.

Acta Physiologiae Plantarum

2014

tom 36

nr 09

The plastidial ω-3 fatty acid desaturase catalyses the production of trienoic fatty acids (TAs) in plant chloroplasts and plays an important role in plant responses to environmental stress. In this study, the full-length cDNAs encoding two plastidial ω-3 desaturases, designated CsFAD7 and CsFAD8 (GenBank Accession No. JX943516 and KC847167, respectively), were isolated from the tea plant (Camellia sinensis L.) using RT-PCR and RACE. Codon usage analysis revealed that U- and A-ended codons were preferentially used in these two genes. Sequence analysis showed that the deduced amino acid sequences of CsFAD7 and CsFAD8 had high homology to plastidial ω-3 desaturases from other plant species. Expression analysis by real-time PCR revealed that both genes are tissue-specific and expressed the highest levels in shoots. Meanwhile, CsFAD7 and CsFAD8 responded to various abiotic stresses and hormones, but in very different manners. Taken together, these results suggest that CsFAD7 and CsFAD8 are both responsive to abiotic stress signals; however, they may play very different roles during stress tolerance in tea plants.

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Mutanty rzepaku ozimego podwojnie ulepszonego o zmienionym skladzie kwasow tluszczowych.

59%

Spasibionek S., Byczynska B., Krzymanski J.

Rośliny Oleiste - Oilseed Crops

2000

tom 21

nr 3

715-724

Elementy nieciaglosci w rozkladzie zawartosci kwasu oleinowego w oleju z nasion linii wsobnych rzepaku ozimego podwojnie ulepszonego

51%

Krzymanski J., Krotka K., Pietka T.

Rośliny Oleiste - Oilseed Crops

2004

tom 25

nr 1

11-26

Ograniczanie wyników

2 Rośliny Oleiste - Oilseed Crops

1 Acta Physiologiae Plantarum

2 Krzymanski J.

1 Byczynska B.

1 Ding Z.-T.

1 Krotka K.

1 Ma Q.-P.

1 Pietka T.

1 Spasibionek S.

1 Wang J.

1 Wang Y.

1 You E.

1 2014

1 2004

1 2000

Wyniki wyszukiwania

Isolation and expression of CsFAD7 and CsFAD8, two genes encoding omega-3 fatty acid desaturase from Camellia sinensis

Mutanty rzepaku ozimego podwojnie ulepszonego o zmienionym skladzie kwasow tluszczowych.

Elementy nieciaglosci w rozkladzie zawartosci kwasu oleinowego w oleju z nasion linii wsobnych rzepaku ozimego podwojnie ulepszonego