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Evaluation of PCR test for detection of dermonecrotoxin of Bordetella bronchiseptica

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Stepniewska K., Markowska-Daniel I.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 4

The aim of the study was the evaluation and optimisation of PCR test for the detection of dermonecrotoxin gene (DNT) of Bordetella bronchiseptica. For the optimisation of the test, vaccine strain B16 was used. The optimisation procedure included: estimation of optimal Mg²⁺ concentration, annealing temperature, numbers of cycles, as well as sensitivity. The specificity of PCR test was checked with DNA of other pathogens existing in pigs' respiratory tract. The elaborated test was specific and sensitive to detect DNT gene of B. bronchiseptica, in both clinical samples, as well as in pure culture of the bacteria.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Markowska-Daniel I.

1 Stepniewska K.

1 2010

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Evaluation of PCR test for detection of dermonecrotoxin of Bordetella bronchiseptica