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It is proposed that it is possible to identify some of the problems that had to be solved in the course of evolution for the red blood cell (RBC) to achieve its present day effectiveness, by studying the behavior of systems featuring different, partial characteristics of its membrane. The appropriateness of the RBC volume to membrane area ratio for its circulation in the blood is interpreted on the basis of an analysis of the shape behavior of phospholipid vesicles. The role of the membrane skeleton is associated with preventing an RBC from transforming into a budded shape, which could form in its absence due to curvature-dependent transmembrane protein-membrane interaction. It is shown that, by causing the formation of echinocytes, the skeleton also acts protectively when, in vesicles with a bilayer membrane, the budded shapes would form due to increasing difference between the areas of their outer and inner layers.
The water content of the rivers is formed by atmospheric precipitation and underground waters. Influence of underground waters on water content of the rivers cannot be measured. It is shown that the volume of underground water exchange is underestimated and can be commensurable with a volume of atmospheric precipitation. Change of level of underground waters is defined by changes of volume of the geological environment during geodeformations. It is offered to consider geodeformations as one of the reasons of floods and droughts. Studied the changes the gravitational field and geodeformations during droughts and floods in the Amazon in 2005-2006. Studied the hydrological regime of the River Nile. Shows the influence of geodeformation on the level of Danube and Dniester. Proposed detailed study the causes of floods in Europe in 2002. Influence of the Earth’s surface deformation on floods and droughts is very important and requires special detailed study. Changes in volume of rocks during Earth’s surface deformation are accompanied by dilatancy which influence on the amount of drought and flooding has turned out to be significant. Study of the processes considered in the thesis gives grounds to expect that floods and droughts associated with deformations of the geological environment will be successfully predicted.
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Endothelial secretogogues and deformability of erythrocytes

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Many diseases of the heart and circulatory system have been linked with both dysfunction of vascular endothelium and insufficient deformability of erythrocytes. Using shear stress laser diffractometry we investigated whether deformability of erythrocytes would be regulated endogenously by generation of two endothelial secretogogues: prostacyclin and nitric oxide. Experiments were performed in rats ex vivo and with whole blood or isolated erythrocytes in vitro. Iloprost - a stable analogue of prostacyclin (10 µg/kg i.v.) and SIN-1 (NO-donor) at a dose of 2 mg/kg/min i.v induced a significant improvement of deformability of erythrocytes ex vivo. Improvements of deformability by these two compounds were also evident in vitro when they were applied at a range of concentrations of 1 µM and 3 µM, respectively. Cyclooxygenase (indomethacin 20 mg.kg i.v.) and nitric oxide synthase (L-NAME 10 mg/kg i.v.) inhibitors while worsening deformability ex vivo, they did not affect (3 mM and 10 µM, respectively) rheological functions of erythocytes in vitro. Aggravating effects of these inhibitors on erythrocyte deformability ex vivo were reversed by prostacyclin and nitric oxide supplemented exogenously. Aspirin at a low (1 mg/kg i.v.) and high dose (50 mg/kg i.v.), contrary to indomethacin and L- NAME, aggravated erythrocyte deformability either ex vivo or in vitro. It is concluded that autocrine function of vascular endothelium plays an important role in regulation of rheology of red blood cells in flowing blood. The mechanism of this phenomenon is unclear but some possible explanations are discussed. In addition, in our experiments aspirin revealed unique erythrocyte damaging properties, possibly independent of inhibition of cyclooxygenase, but related to a direct protein acetylation.
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